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Food chemistry
Applied Science Publishers Ltd.
Food chemistry

Applied Science Publishers Ltd.

0308-8146

Food chemistry/Journal Food chemistrySCIEI
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    Thermal-stabilized nanoflower luciferase-based detection of SCC and TPC in raw milk

    Yang L.Fan Y.Li X.Sun X....
    1.1-1.10页
    查看更多>>摘要：© 2025 Elsevier Ltd.Simultaneous determination of Somatic Cell Count (SCC) and Total Plate Count (TPC) in raw milk reflects milk cleanliness and cow health. The luciferase was thermally unstable and required storage at −20 °C, with repeated freeze-thaw cycles reducing activity. To overcome this limitation, an inorganic hybrid nanoflower luciferase was synthesized by mixing genetically engineered free luciferase with PBS and zinc acetate, followed by incubation, centrifugation, washing, and resuspension. Transmission electron microscopy confirmed successful assembly of luciferase into nanoflower structures. The nanoflower luciferase remained stable over 75 % of its initial activity after 21 days at 4 °C, whereas free enzyme required −20 °C.Compared with genetically engineered free luciferase, the nanoflower form effectively and simultaneously detected SCC and TPC in raw milk even after 2-fold dilution. The limits of quantification were 1 × 104 cells/mL for SCC and 1 × 105 CFU/mL for TPC.
      原文链接:
    • NETL
    • NSTL
    • Elsevier

    Cili pomace-enhanced chitosan/cellulose nanofibril composite films for antioxidant, antibacterial, and biodegradable food packaging

    Ma H.Qu M.Yang F.Zhou X....
    1.1-1.13页
    查看更多>>摘要：© 2025 Elsevier Ltd.Cili ( Rosa roxburghii Tratt .) pomace is rich in bioactive components, making it a promising additive for functional packaging materials. Cili pomace extracts (CLPE) were incorporated into chitosan/cellulose nanofibril (CS/CNF) matrices to develop a food packaging material—strong intermolecular hydrogen bonding among CS, CNF, and CLPE, leading to amorphous structural features. The CS/CNF/CLPE composite films, particularly those with 20 % CLPE loading, displayed significantly reduced water vapour permeability (WVP), enhanced thermal stability, and superior UV-blocking capacity. Additionally, the enrichment of CLPE endowed the composite films with potent functional properties, enhancing their antibacterial efficacy against Escherichia coli and Staphylococcus aureus . In strawberry preservation tests, the CS/CNF/20 %CLPE film solution inhibited decay, minimised weight loss, and maintained fruit quality. Additionally, films containing CLPE accelerated biodegradation, with over 70 % degradation occurring within 21 d. Therefore, the multifunctional CS/CNF/CLPE composite film, which leverages the valorisation of agricultural by-products, presents a promising sustainable packaging candidate for active food preservation.
      原文链接:
    • NETL
    • NSTL
    • Elsevier

    Advancing olfactory perception research with EEG analysis: a dynamic approach of understanding brain responses to almond deterioration

    Li J.Han J.Chen S.Li Q....
    1.1-1.9页
    查看更多>>摘要：© 2025 Elsevier Ltd.Electroencephalography (EEG) enables the investigation of olfactory perception through neuronal electrical activity. Decoding dynamic oscillatory changes in sensory-cognitive processing is critical to understanding odor-induced brain responses. First, the EEG signals of almond were obtained and transformed into the frequency domain. Welch's method was implemented to extract power spectral density (PSD). Subsequently, the power spectral analysis of brain responses across different regions and frequency bands was investigated. Moreover, the machine learning approach was employed to explore the primary discriminative features. As a result, pronounced oscillatory activity was obtained in delta and alpha bands inducing distinct spatial-frequency responses of increased δ-power in left temporal region and β-power in parieto-occipital region. Critically, the β-band frequencies of 18 Hz and 25.5 Hz, and channels of FP2, FZ, and C3 were confirmed as key features contributing to olfactory analysis. This study provides valuable insights for olfactory perception and applications for quality assessment and storage monitoring.
      原文链接:
    • NETL
    • NSTL
    • Elsevier

    Gut microbiota modulation by polysaccharide from Aloe gel: Potential prebiotic and targeted regulatory effects

    Sun Y.Zhang S.Nie S.
    1.1-1.9页
    查看更多>>摘要：Copyright © 2024. Published by Elsevier Ltd.The human gut microbiota has gained interest as a crucial factor contributing to health. Aloe gel polysaccharide (AGP) significantly impacts gut microbiota structure and function, yet its rule remains unclear. Here, we collected feces from 30 healthy volunteers and performed in vitro fermentation. We characterized the influence of AGP on gut microbiota using multi-omics analysis, identified key species, and conducted monoculture experiments to clarify their relationship with AGP. Firstly, AGP suppressed the potentially harmful bacteria and the accumulation of branched-chain fatty acids, p-cresol, and indole, while enriching potential beneficial bacteria and the accumulation of short-chain fatty acids. Moreover, AGP specifically enriched the proliferation of Faecalibacterium prausnitzii , Parabacteroides distasonis , and Bacteroides uniformis . Monoculture showed that AGP can be degraded by B. uniformis , not F. prausnitzii and P. distasonis . This work lifts a corner of AGP in modulating the gut microbiota, providing a reference for the development of AGP-based functional foods.
      原文链接:
    • NETL
    • NSTL
    • Elsevier

    Influence mechanism of fixation on the development of green tea flavor and related non-volatile and volatile metabolites by objective quantification, widely targeted metabolomics, and aroma metabolomics

    Hua J.Li X.Zou C.Yang L....
    1.1-1.15页
    查看更多>>摘要：© 2025 Elsevier Ltd.Fixation is key for green tea flavor formation; however, the impact of fixation methods on non-volatile metabolites (NVMs) and volatile metabolites (VMs) regulation remains unclear. We applied objective quantification techniques, widely targeted metabolomics, and aroma-omics to analyze the influence of fixation methods on green tea quality. Roller-hot air coupling fixation (RHF) produced an umami taste with a chestnut aroma, whereas roller-steam coupling fixation (RSF) produced a tender aroma with bitterness. We detected 498 NVMs and 63 VMs, and 14 key quality-differential NVMs and 7 key VMs were identified. KEGG revealed flavonoid, amino acid, glycoside, and carotenoid metabolism were the key pathways caused by fixation methods resulting in green tea flavor difference. RHF accelerated flavonoid transformation and amino acid formation metabolism, resulting in higher L-glutamic acid and catechin contents, lower rutin and l -lysine contents. These results provide a theoretical and technical support for precise targeted processing of high-quality green tea.
      原文链接:
    • NETL
    • NSTL
    • Elsevier

    Covalently and noncovalently embedding regenerated silk fibroin/polyphenol nanoparticles in sodium alginate-based cryogels for enhanced antioxidative and antibacterial activities

    Jiang Q.Zhang H.Sun Q.Peng Y....
    1.1-1.11页
    查看更多>>摘要：Copyright © 2025. Published by Elsevier Ltd.Foods with high-starch porous structures have a high glycemic index (GI), posing health risks. Replacing traditional starch networks with dietary fiber-based porous skeletons promotes a low-GI diet. Herein, regenerated silk fibroin (RSF) and polyphenol complex nanoparticles were embedded into the sodium alginate (SA) cryogel by covalent and noncovalent approaches. Nanoparticles were fabricated by covalent interactions between RSF and polyphenols (proanthocyanidin (PA) and quercetin (QUE)) using a bottom-up method under alkaline conditions. Heating and non-heating treatment on pre-solutions caused Maillard reactions and hydrogen bonding between nanoparticles and the SA matrix. The noncovalent embedding of RSF-PA nanoparticles greatly improved the mechanical properties of the cryogels, with the compressive strength increasing from 24.41 to 139.83 kPa. Cryogels with covalently embedded nanoparticles had higher polyphenol release rates, antioxidation, and antibacterial activities (11.2 mm of inhibition zone against S. aureus ). This work promotes the development of low GI functional diets from dietary fiber-based porous matrices.
      原文链接:
    • NETL
    • NSTL
    • Elsevier

    Rational screening of anti-E. coli peptides via GAT-MD integration leveraging foodborne antimicrobial sequence features

    Wang Q.Ma Q.Chen S.Han K....
    1.1-1.14页
    查看更多>>摘要：Copyright © 2025. Published by Elsevier Ltd.Antimicrobial peptides (AMPs) offer promising alternatives to conventional antibiotics due to their broad-spectrum activity and multi-modal mechanisms. However, large-scale experimental screening remains costly and time-consuming. To expedite AMP discovery, this study proposes a combined screening strategy based on Graph Attention Networks and molecular dynamics simulations. Utilizing a food-derived bioactive peptide database, the method efficiently identified candidate AMPs with activity against Escherichia coli ATCC 25922 and methicillin-resistant Staphylococcus aureus ATCC 33591 from a pool of 100,663,296 peptides. Experimental validation confirmed that the identified AMP, AMP_321, exhibited broad-spectrum antimicrobial activity. The peptide demonstrated excellent stability and low hemolytic activity. Mechanistic studies revealed that AMP_321 exerts its antibacterial effect by disrupting bacterial membrane integrity, inducing extracellular ATP release, and promoting intracellular accumulation of reactive oxygen species. In food models, AMP_321 significantly reduced bacterial contamination on beef and tomato surfaces, validating its potential as a safe and effective natural food preservative.
      原文链接:
    • NETL
    • NSTL
    • Elsevier

    Ginger as a novel starch resource: Preparation, structural characterization and physicochemical properties

    Pan Y.Wang C.Geng S.Liu B....
    1.1-1.13页
    查看更多>>摘要：© 2025 Elsevier Ltd.This study systematically investigated the structural characteristics and physicochemical properties of ginger starch, comparing them with those of corn starch (CS). The results revealed that ginger starch granules exhibited elongated oval and round-like shapes, with a smooth and flat surface, birefringence, an A-type crystalline structure, and a smaller particle size than CS. Compared to CS, ginger starch had higher amylose (31.16 ± 0.39 %) and post-cooking resistant starch content (15.54 ± 0.58 %), longer average chain length of amylopectin, and higher crystallinity (24.83 %). However, its weight-average molecular weight, radius of gyration, degree of branching, and short-range ordered structure were lower than those of CS. In terms of functional properties, ginger starch demonstrated excellent thermal stability and shear processing tolerance, though its overall functional properties remained inferior to CS. Notably, ginger starch had significantly higher total phenolic content, exhibiting greater antioxidant activity. These findings provide insights for developing new starch resources and advancing ginger processing and utilization.
      原文链接:
    • NETL
    • NSTL
    • Elsevier

    Responses of Chinese olives to low-temperature storage at different harvest dates involve the metabolisms of ROS, membrane lipids, and phenolics

    Sang Y.Zhou H.Lin Z.Huang G....
    1.1-1.15页
    查看更多>>摘要：© 2025 Elsevier Ltd.This work evaluated the influences of harvest dates on chilling injury (CI) and the metabolism of reactive oxygen species (ROS), membrane lipids, and phenolics in Chinese olives. Among the tested dates (I, III, V, VII), determined by Chinese solar terms and maturity indicators, Chinese olives harvested on date V exhibited the lowest levels of CI, browning, O2·-, and MDA, alongside the highest levels of antioxidant enzymes and substances. Moreover, Chinese olives harvested on date V displayed the lowest activity of membrane lipid-degrading enzymes and maintained higher unsaturation of membrane fatty acids. Also, these Chinese olives displayed the lowest activities of PPO and POD, while exhibiting the highest levels of PAL, flavonoids, and total phenolics. These findings imply that harvest date V (Start of winter, 7 November) is optimal for minimizing CI in Chinese olives by improving antioxidant capacity, reducing cell membrane degradation, and mitigating phenolic oxidation during cold storage.
      原文链接:
    • NETL
    • NSTL
    • Elsevier

    Pecan shell phytochemicals: chemical fingerprint, agro-industrial applications and delivery systems

    Siebeneichler T.J.Peixoto E.C.Rombaldi C.V.Dias A.R.G....
    1.1-1.14页
    查看更多>>摘要：Copyright © 2025. Published by Elsevier Ltd.Global pecan production has increased, with consumption focused on the kernel, while the shells (endocarp) remain underutilized. This review presents the chemical composition of pecan shells and discusses their agro-industrial applications and delivery systems. A literature search in the Web of Science using the term “pecan shell” retrieved 236 documents, analyzed using bibliometric indicators and keyword co-occurrence mapping. Pecan shells are rich in phenolics, melanins, insoluble fibers, and minerals, with notable antioxidant, antimicrobial, and antifungal properties. Their main bioactive potential lies in antioxidant action, especially in lipid product preservation, as well as pathogen inhibition, partial replacement in bakery products, and development of active packaging. Technologies like zein-based microencapsulation via spray-drying, and electrospun melanins have been investigated to enhance compound stability and delivery. Although progress has been made, over 90 % of the biomass remains as solid waste, which can be converted into by-products such as biochar, contributing to circular bioeconomy strategies.
      原文链接:
    • NETL
    • NSTL
    • Elsevier