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Food research international
Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science
Food research international

Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science

0963-9969

Food research international/Journal Food research internationalSCIEI
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    1H NMR multisuppression approach for rapid detection and quantification of acyl groups and fatty acids supporting cyclopropane structures, either natural or generated during severe heating

    Nieva-Echevarria B.Ruiz-Aracama A.
    1.1-1.14页
    查看更多>>摘要:© 2025 The AuthorsThis study has demonstrated the usefulness of 1H Nuclear Magnetic Resonance (1H NMR) multisuppression to investigate the potential occurrence of acyl groups/fatty acids supporting cyclopropane structures (AG/FA-3C) in commercial edible oils. An array of virgin and refined oils from 13 botanical origins was acquired and their 1H NMR spectra, particularly the upfield region, studied. Although not previously described in edible oil spectra, several highly shielded signals corresponding to AG/FA-3C could be easily detected and quantified. Some of them were associated with cyclopropane-containing saturated AG/FA of natural origin resulting from plant metabolism. This was the case of lactobacillate or dihydrosterculate, whose characteristic quadruplet at −0.33 ppm was only found in rapeseed and cottonseed oil samples, being thus potential useful markers of their botanical origin. By contrast, other highly shielded proton signals were related with modified polyunsaturated AG/FA generated during severe heating. Indeed, these latter were only visible in the spectrum of the edible oils rich in polyunsaturated acyl groups if refined. Moreover, the profile of signals appearing between −0.4 and 0.5 ppm differed in the case of linoleic or linolenic-rich oil thermodegradation. The employed 1H NMR approach enabled to gain insight into the composition of commonly consumed edible oils, providing information about these rare lipid components in a simple and fast way, without any chemical modification of the sample prior analysis. Additionally, in the case of polyunsaturated oils, the study of the upfield region of the 1H NMR spectrum may be useful for a rapid discrimination between virgin and refined samples.

    Analysis of pesticide residues in açaí-based food products: an approach using polymeric ionic liquid-based solid-phase microextraction coupled to gas chromatography–mass spectrometry

    Nascimento L.E.S.Galindo M.V.Godoy H.T.Thapa B....
    1.1-1.12页
    查看更多>>摘要:© 2025 Elsevier LtdThe market for açaí-based food products (AFPs) has been growing steadily, particularly within the international sector. The Brazilian Health Regulatory Agency (ANVISA) authorizes the use of certain pesticides in açaí cultivation, which may result in residues in derived products. A solid-phase microextraction (SPME) method based on polymeric ionic liquid (PIL) sorbent coatings coupled with gas chromatography–mass spectrometry (GC–MS) was developed and validated for the determination of pesticide residues in AFPs. A multivariate optimization was performed that focused on the following three variables: extraction temperature, extraction time, and sodium chloride concentration within the extraction solution. The optimized and validated method yielded satisfactory results, with limits of quantification ranging from 1 to 9 μg kg−1, linearity with R2 ≥ 0.987, recoveries between 70 % and 88 %, and precision ranging from 2 % to 12 %. The matrix effect exhibited suppression up to 87 % and enhancement up to 22 %. Furthermore, the method's greenness and practicality were assessed using established metrics. The results demonstrated that the method can be classified as both practical and environmentally-friendly. In the occurrence analysis, S-metolachlor was quantified in four samples at concentrations ranging from 1.1 to 1.3 μg kg−1. Thiabendazole and fluxapyroxad were detected in concentrations below the limit of quantification. In addition, all detected pesticide levels were below their respective acceptable daily intake values. This method demonstrated robust performance for the quantification of pesticides in AFPs and provides important data for regulatory agencies regarding pesticide residues in açaí-derived products.

    Reducing cellular inflammation and oxidation by supercritical CO2 extracts from edible-medicinal mushrooms

    Siles-Sanchez M.D.L.N.Garcia-Ponsoda P.Santoyo S.Ruiz-Rodriguez A....
    1.1-1.11页
    查看更多>>摘要:© 2025 The AuthorsExtracts from Hericium erinaceus, Ganoderma lucidum and Tremella fuciformis obtained with supercritical CO2 showed high cellular antioxidant activity (CAA) when tested on Caco-2 cell cultures. Extract obtained in separator 2 (5 MPa) after 3 h extraction at 30 MPa and 40 °C from G. lucidum showed a remarkable higher CAA than noticed for the other species. In addition, extracts to THP-1 macrophages reduced secretion of TNF-α and particularly IL-6 (80–90 % applied at 15 μg/mL). The G. lucidum extract was also more effective than those from the other two species except for IL-1β. The use of ethanol 5 % (v/v) as co-solvent for extractions increased the yield but reduced both activities. Extracts from studied species contained linoleic, oleic, α-linolenic and palmitic acids as the main fatty acids, and ergosterol and ergosterol peroxide (EP) as fungal sterols. Those obtained from G. lucidum included ganoderic acids A and D, ganoderol B and lucidenic acid A. When identified compounds were tested, ganoderol B, ganoderic acid A, linoleic acid, α-linolenic acid and EP showed remarkable CAA. The anti-inflammatory activities of α-linolenic and linoleic acids were higher than those noticed for ergosterol and EP, and the latter compounds were more effective than the other tested triterpenoids. These results suggest that the anti-inflammatory effect noticed (particularly for G. lucidum extract) might be due to a synergistic activity of the indicated compounds.

    Spermidine enhances cold tolerance in tomato fruit by boosting antioxidant and energy metabolism synergy

    He J.-L.Xie S.-Y.Fu D.-Q.Zhao X.-D....
    1.1-1.12页
    查看更多>>摘要:© 2025 Elsevier LtdCold stress is a major factor affecting postharvest quality of tomato fruit. Although polyamine compounds have been widely used to enhance plant cold tolerance, direct evidence supporting spermidine (SPD) involvement in regulating tomato fruit cold resistance is still lacking. In this study, tomato fruits were treated by SPD or its inhibitor Methylglyoxal bis guanylhydrazone (MGBG), followed by storage at 4 °C, 25 °C, or a combination of both temperatures. Cold injury (CI) levels, along with relevant indices of the antioxidant and energy metabolism systems, were evaluated. The results showed that SPD significantly enhanced cold resistance in tomatoes by activating the antioxidant and energy metabolism systems. Furthermore, correlation analysis and transcriptome sequencing indicated a synergistic interaction between the antioxidant and energy metabolism systems, which jointly regulate cold tolerance in tomato fruit, with SPD further enhancing this synergy.

    A green and efficient deep eutectic solvent for extracting total flavonoids from Artemisiae scopariae Herba (Yin Chen): chemical characterization, antioxidant activity, and hepatoprotective effects, and mechanisms of action against liver injury

    Wang Y.Zhang Y.Gu M.Zhang J....
    1.1-1.18页
    查看更多>>摘要:© 2025This study developed the first deep eutectic solvents (DESs) system specifically for extracting total flavonoids from Artemisiae Scopariae Herba (ASHTF), with documented hepatoprotective potential. We optimized the extraction process of DES and comprehensively evaluated its advantages over ethanol extracts (TOS extract) through both in vitro and in vivo experiments. In vitro, chemical characterization was performed using UPLC-Q-Exactive Orbitrap MS, followed by antioxidant activity assessment. In CCl4-induced liver injury mice, the hepatoprotective effects of two extracts were compared, and the mechanism by which DES extract ameliorates liver injury was clarified through a combination of 16S rRNA gene sequencing and fecal metabolomics analysis. When choline chloride/1,2-propanediol were used as extraction solvent, the extraction efficiency of ASHTF demonstrated a 27 % increase compared to ethanol. Twenty-six flavonoids were identified in the DES extract—five more than in the TOS extract—with stronger antioxidant activity. In vivo, the DES extract demonstrated superior efficacy compared to TOS in mitigating hepatic damage, inflammation, and oxidative stress. Mechanistically, DES extract modulated the composition of gut microbiota and reversed the changes of fifty-four fecal metabolites in mice with liver injury, particularly regulating pyrimidine metabolism, β-alanine metabolism, and glycerophospholipid metabolism. DES extraction outperforms TOS in extraction efficiency, composition, antioxidant activity, and hepatoprotective efficacy. Overall, DES not only augmented the extraction efficiency of ASHTF and effectively preserved its biological activity but also reduced solvent waste to lower costs and mitigated TOS's environmental pollution risks. This provides a theoretical foundation for the extraction of natural products using DES and holds practical significance.

    Dietary contribution of essential elements from honey consumed in the United States

    Stoner H.T.V.Nyachoti S.Taylor P.K.Abdelghani A....
    1.1-1.14页
    查看更多>>摘要:© 2025Honey is a natural sweetener with nutritional properties, contributing minerals to the human diet. Data on U.S. honey is limited. This study analyzed the concentrations of 13 essential elements (B, Ca, Cu, Fe, K, Mg, Mn, Mo, Na, Ni, Se, Sr, and Zn) in 261 commercially available single-source honey samples from the U.S. using Inductively Coupled Plasma Mass Spectrometry (ICP-MS). The study assessed nutritional contributions, regional variations, and non-carcinogenic risks from honey consumption. On average, Se, Mo, Ni, Sr, and Cu ranged from 3.3 to 255 μg/kg, whereas Zn, Mn, Fe, B, Mg, Na, Ca, and K ranged from 0.70 to 615 mg/kg. For macroelements, K was the most abundant element, followed by Ca, with B, Fe, and Mn being highest among the microelements. Spearman correlation, Principial Component Analysis (PCA), and Kruskal–Wallis tests revealed significant inter-element relationships. Potassium and Mg were strongly correlated (r = 0.71), reflecting co-mobilization in plant nectar secretion. Moderate correlations were observed among Mg, Ca, Cu, Zn, Fe, Mo, Na, Mn, and Sr (r = 0.40–0.65), due to shared botanical and environmental influences. While significant differences between regions were identified by the Kruskal–Wallis test, PCA results showed no distinct regional clustering, indicating that the essential elemental composition of U.S. honey is not significantly influenced by geographic origin. Dietary Reference Intake (DRI) contributions were estimated for three intake levels (2 g, 21 g, and 42 g/day). At the highest level, honey provided up to 10.2 %, 11.3 %, and 16.5 % of DRI for Cu, B, and Mn, respectively, for young children. Contributions for other elements were negligible across serving sizes. Non-carcinogenic risk assessment indicated no health concerns at any intake level. This study examined the elemental composition of U.S. honey and its role as a safe, minor source of essential minerals.

    BfrR-mediated biofilm regulation in the seafood-borne pathogen Vibrio parahaemolyticus

    Sun H.Zhang M.Xiong S.Li X....
    1.1-1.11页
    查看更多>>摘要:© 2025 Elsevier LtdVibrio parahaemolyticus is a major seafood-associated pathogen whose biofilm formation enhances environmental persistence, antibiotic resistance, and food safety risks. In this study, we identified a LysR-type regulator, BfrR (VPA1687), as a critical mediator of biofilm development in V. parahaemolyticus. Deletion of bfrR severely impaired biofilm formation, switching colony morphology from wrinkled to smooth and reducing extracellular matrix components (exopolysaccharides (EPS), proteins, and extracellular DNA). BfrR also modulated metabolic activity in biofilm-embedded cells and exhibited dual control over motility: it inhibited swimming while promoting swarming. Mechanistically, BfrR elevated intracellular c-di-GMP levels by regulating genes encoding GGDEF/EAL domain proteins. RNA-seq revealed BfrR influences 500 genes, including biofilm-related pathways (e.g., cps operon for EPS), virulence factors, and flagellar genes. Critically, BfrR was essential for biofilm formation on diverse food-contact surfaces (shrimp, crab, stainless steel, plastic, and glass). These findings establish BfrR as a global regulator that coordinates biofilm formation, motility, and c-di-GMP signaling, highlighting its potential as a target for mitigating V. parahaemolyticus biofilm-associated risks in the food industry.

    CRISPR–Cas systems as emerging tools for precision biofilm control for food safety: Mechanisms and applications

    Rahman M.A.Akter S.Ashrafudoulla M.Ha S.-D....
    1.1-1.15页
    查看更多>>摘要:© 2024Biofilms on food-contact surfaces pose persistent challenges to sanitation, safety, and product quality within food processing. Traditional cleaning methods and broad-spectrum antimicrobials often fail to disrupt the resilient matrix and multispecies communities characteristic of these biofilms. Clustered regularly interspaced short palindromic repeat (CRISPR)–CRISPR-associated (Cas) systems offer a transformative approach to enhancing food safety, enabling precise modulation of microbial gene networks with applications in diagnostics, programmable sanitation, and targeted microbial control. This review synthesizes recent advances in CRISPR–Cas technology, encompassing Cas9/Cas12-based gene editing, Cas13-mediated RNA targeting, and dead Cas9 (dCas9)-based transcriptional regulation (CRISPR interference/activation, CRISPRi/a), and evaluates their relevance to biofilm prevention and eradication in food environments. We critically assess delivery platforms, including plasmids, nanocarriers, phagemids, and conjugative systems, for their efficiency in complex biofilm settings. The review highlights innovations such as multiplexed repression of redundant pathways, activation of latent antibiofilm functions. These genetic strategies are increasingly being integrated with omics-based analytics (e.g., transcriptomics, proteomics, metabolomics) to reveal systems-level cellular responses and regulatory shifts triggered by biofilm-targeted interventions. We also address the practical limitations, such as delivery barriers, off-target effects, regulatory hurdles, and ethical considerations specific to food applications. Ultimately, we propose a framework for translating CRISPR–Cas technology into scalable, safety-compliant tools for precision control of biofilms in food processing environments. This review aims to guide future research and inform stakeholders on leveraging CRISPR–Cas technology for safe, sustainable, and targeted management of food-associated biofilms.

    Absolute quantitation of celiac disease-active gluten peptides in gluten-free barley beer by targeted nanoLC-MS/MS

    Tissen E.Scherf K.A.Geisslitz S.
    1.1-1.10页
    查看更多>>摘要:© 2025 The AuthorsGluten-free barley beers may cause symptoms in celiac disease (CeD) patients due to CeD-active peptides from partially hydrolyzed gluten. Accurate quantitation of such peptides is essential to improve analytical methods for assessing CeD immunogenic activity and ensuring consumer safety, as routine methods like R5 competitive enzyme-linked immunosorbent assays (R5c ELISA) may miss small yet harmful peptides. This study used targeted nano-liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) with stable isotope dilution assay to quantitate seven CeD-active peptides with complete immunogenic epitopes in 19 gluten-free and four regular or carbohydrate-reduced beers. The selection of CeD-active peptides was made based on their previous identification in the analyzed beers. The beers were subjected to single-pot solid-phase-enhanced sample preparation and chymotryptic hydrolysis. All peptides were under the limit of quantitation in 13 commercially available gluten-free beers. In the remaining six, the total peptide concentrations ranged from 14 to 806 ng/mL, while non-gluten-free beers contained 967–3089 ng/mL. Peptide concentrations correlated with the gluten content measured by R5c ELISA, and lot-to-lot variations were observed. These findings support the need for a more comprehensive risk assessment of products containing partially hydrolyzed gluten.

    Colloidal design and interfacial engineering in coaxial 3D printed functional foods: from material assembly to personalized nutrition delivery

    Huang J.Zhang M.Li C.Mujumdar A.S....
    1.1-1.16页
    查看更多>>摘要:© 2025 Elsevier LtdCoaxial 3D printing technology, as an additive manufacturing technology, has been widely used in the fields of materials, biomedicine and intelligent manufacturing through synchronized multi-material extrusion and structure control. In the field of food, it can realize the embedding, controlled release and personalized customization of nutritional components of functional food, which has received great attention. However, the systematic research on this technology in colloidal design and interfacial interactions is still insufficient. This paper starts from the logic that material properties influence the process, the process promotes the realization of function, and the functional requirements in turn guide the material innovation. Firstly, the types of materials suitable for coaxial 3D printing and pre-treatment techniques, along with the interaction mechanisms at multi-material interfaces are analyzed. These are the material foundation for process optimization. Second, the methods for optimizing key process parameters and strategies for balancing the structure and functionality of printed products are explored. In addition, the design of texture and swallowing adaptability, sensory quality enhancement, and controlled release methods of nutrient components encapsulated in core-shell structures are discussed. Finally, the current status and challenges of the technology are analyzed and its potential future applications are envisioned. Overall, coaxial 3D printing holds significant potential for revolutionizing personalized functional food production.