Min, Ju HyunAminu, Mariam OmowunmiKang, Hye JeeLee, Yeon Ju...
1.1-1.12页
查看更多>>摘要：A hybrid hydrogel composed of konjac glucomannan (KG) and agar (AG) was developed for the efficient adsorption of peptides derived from casein hydrolysate. Hydrogel matrix was formulated with different concentrations of KG and AG, and the optimal composition was determined on the basis of morphologies, structural properties, rheological behaviors, and mechanical characteristics. Among the tested formulations, the hydrogel containing 2.5 % KG and 1.5 % AG (2.5KG-1.5AG) exhibited significantly higher gel strength, gel content, swelling index, and hardness compared to the 3.0KG-1.0AG formulation. Moreover, 2.5KG-1.5AG exhibited more favorable surface characteristics, including a uniform porous structure, larger surface area, and enhanced peptide adsorption capacity. This formulation achived a maximum peptide adsorption capacity of 113.59 mg/g for peptides from a 150 mg/L casein hydrolysate solution. Adsorption kinetics followed a pseudo-second-order kinetics model, implying a chemisorption mechanism, while Freundlich isotherm suggested multilayer adsorption on a heterogeneous surface. In addition, 2.5KG-1.5AG showed excellent desorption properties (>93 %), suggesting its potential for reusability and operational stability. These findings confirm that the KG-AG hybrid hydrogel represents an effective and sustainable bio-based adsorbent for the selective separation of peptides. Its cost-efficiency, high adsorption capacity, and remarkable reusability support its applicability in food-grade bioactive peptide enrichment and purification systems.
Salgado-Aristizabal, NataliaGalvis-Nieto, Juan D.Abad, VanesaNarvaez-Perez, Jorge M....
1.1-1.15页
查看更多>>摘要：The rocoto chili pepper (Capsicum pubescens) is a lesser-known domesticated species of chili pepper, valued for its pungency and aromatic properties. Chili peppers are regarded as functional foods due to bioactive phytochemicals. Various drying techniques are employed to extend the relatively short shelf life of these peppers, including oven drying, natural convection drying, solar drying, and smoke drying. Nevertheless, no studies have yet examined the production of chili powders by encapsulating the fruit pulp using drying techniques. This study employed three agro-industrial products (maltodextrin, rice flour, and rice protein), which are inexpensive and readily available in Colombian chili-producing regions, to safeguard the capsaicinoids, polyphenols, and flavonoids present in the chili pulp. The encapsulated chili pulp powders were subjected to analysis for capsaicinoid content, other bioactive compounds (total phenols, flavonoids, and capsaicinoids), and antioxidant activity. Similar measurements were made for vacuum-dried chili pulp without encapsulants. The study also examined the evolution of bioactive concentrations under ultraviolet-C light and incandescent light irradiation, as well as the microbiological quality before and after UV-C irradiation treatments.
Botelho, Victor RodriguesMonte, Micheli LegemannFilho, Renato Dutra PereiraPinto, Luiz Antonio de Almeida...
1.1-1.8页
查看更多>>摘要：The drying of hop residues is a sustainable alternative for managing agro-industrial waste while preserving bioactive compounds. This study aimed to automate the drying process using Internet of Things (IoT) concepts, analyzing total phenolic compounds, alpha-acids, and R-acids, and evaluating variables with machine learning. Two systems were developed: one for acquiring temperature and moisture content data using sensors and a micro-controller, and another for weighing by capturing images of the balance. Data were transmitted and processed remotely. The drying operation was performed using a heat pump dryer and a tray dryer at 50 degrees C, 60 degrees C, and 70 degrees C until reaching 10 % product moisture content. Spectrophotometric analysis and ASBC methods showed average values of 37.14 mg GAE/g (phenolics), 7.07 % (alpha-acids), and 5.47 % (R-acids), with no significant differences between drying conditions. The IoT and machine learning approach proved efficient for remote monitoring and process automation, enabling real-time supervision and operational alerts.
查看更多>>摘要：This study focused on enhancing the stability and bioaccessibility of pterostilbene (PTS) by encapsulating it with hydroxypropyl-beta-cyclodextrin (HP-beta-CD) using high-pressure homogenization (HPH) and pH-shift methods. The encapsulation process was performed at pressures of 50, 100, and 150 MPa along with three different treatments, achieving a maximum encapsulation efficiency of 94.34% and a loading capacity of 14.82% at 150 MPa. The SEM, XRD, and FTIR analyses confirmed encapsulation by revealing significant morphological changes, crystal structure modifications, and host-guest interactions. PTS-HP-beta-CD complexes retained 82.22% of PTS at 4 degrees C (28 days) and 76% under UVB (120 min), compared to 56.03% and 21% for free PTS, respectively. Additionally, these complexes exhibited enhanced DPPH scavenging rates (44.95% at 0.02 mg/mL), nearly twice that of free PTS. In the release studies, the complexes' release behavior aligned with the Higuchi model. Encapsulated PTS revealed lower digestion rates in in-vitro gastric (32.24% vs. 49.88 %) and intestinal phases (63.98% vs. 91.01%). Additionally, there was no observable toxicity for PTS-HP-beta-CD to human epidermal keratinocytes, supporting its potential use in cosmetics, food, and pharmaceuticals. These results demonstrated that HPH combined with pH-shift methods effectively improved the stability and bioaccessibility of PTS, offering a promising strategy for its formulation.
Penafiel-Alvarado, J. I.Miranda-Pinzon, M.Ivorra-Martinez, J.Stefani, P. M....
1.1-1.12页
查看更多>>摘要：The manufacturing of rice protein (RP) thermoplastic materials by combining it with urea, glycerol, sorbitol and xylitol by extrusion and injection molding techniques are studied in this work. Very promising protein-based sustainable polymeric materials are obtained, with tensile strength superior to 17 MPa in the case of samples plasticized with urea, which allows to obtain very rigid materials. On the other hand, the combination of RP and urea with glycerol, sorbitol or xylitol gives rise to materials with elongation at breaks superior to 40 %. This means a wide range of properties depending on the combination of plasticizers used. Urea crystals finely embedded in the RP matrix are observed by microscopy analysis, which are confirmed by the presence of a melting peak in DSC at approximately 127 degrees C. Chemical analysis also revealed the predominance of these urea crystals through chemical analysis. Dynamic-mechanical thermal analysis (DMTA) also showed the glass transition temperature (Tg) of the materials to be between 28 and 60 degrees C, being the sample with glycerol the one with the lowest value. All materials were observed to be completely biodegradable at 3-4 weeks, gaining increasing environmentally friendly value. The plenty availability of rice protein makes these materials very attractive for producing environmentally friendly materials with great potential in the food industry.
查看更多>>摘要：Given the widespread consumption and high perishability of salmon, real-time and accurate freshness monitoring is crucial for ensuring food safety and reducing economic losses. In this study, two pH indicators, curcumin (CUR) and alizarin (AL), were used to make a dual colorimetric label indicator with polyvinyl alcohol (PVA) and sodium alginate (SA) as the film-forming substrates. This indicator effectively detects quality changes during salmon storage and exhibits vivid color responses. By integrating the dual colorimetric label indicator with deep learning, we trained and tested the lightweight convolutional neural network (CNN) model MobileNetV2, achieving a detection accuracy of 98.59 %. Additionally, we selected SqueezeNet, a lightweight CNN with an inference time of approximately 14 ms, for real-time and high-throughput detection. To deploy these models, we developed a smartphone application (APP) with a user-friendly interface and multiple detection functionalities to meet the needs of professional and non-professional users in various practical scenarios. The dual colorimetric label indicator yields enhanced information and greater accuracy over the single-label approach. Moreover, lightweight CNNs offer novel ideas for freshness detection. Their integration with intelligent packaging through a mobile app enables rapid and non-destructive detection. The research results may hold significant potential in food safety and quality control.
查看更多>>摘要：Porosity and permeability are critical physical parameters for accurately modelling macroscale heat and mass transfer processes during the cooling, thermal processing, and storage of leafy vegetables. However, existing estimation methods primarily rely on lumped semi-empirical approaches, which overlook the realistic 3D structural information, limiting insights into microscale water transport behaviour. This study utilised low-and high-resolution X-ray computed tomography (CT) combined with advanced cell segmentation techniques to determine the porosity-permeability correlation of spinach, a representative easily dehydrated leafy vegetable. Experiments demonstrated that the Cellpose, dilation, and erosion algorithms effectively segmented adhering cells and generated lamina and petiole slices with varying porosity gradients. Using 3D reconstruction and seepage simulation, the porosity and permeability of representative elementary volumes (REVs) in the lamina and petiole tissues were calculated, and the pressure and flow rate distributions within the intercellular spaces were visualised. The porosity-permeability relationship was fitted using the Kozeny-Carman (KC) formula as Kl = 4.839 x 10-11rp1.70/(1-rp)0.70 for lamina REVs and Kp = 1.128 x 10-10rp2.16/(1-rp)1.16 for petiole REVs. Grayscale-porosity and porosity-permeability correlations were further applied to characterise the heterogeneity of porosity (2.742%-53.30%) and permeability (4.925 x 10-14-2.829 x 10-11 m2) of intact spinach. The study aims to provide technical and theoretical support for multiscale modelling in the quality control of leafy vegetables.
Steffens, QuintenVliek, Marijevan der Sman, RuudBoom, Remko...
1.1-1.11页
查看更多>>摘要：Proteins can be plasticised to create thermoplastic materials suitable for 3D printing using fused deposition modelling (FDM). This study explores the plasticisation of zein, calcium caseinate, and their blends with glycerol, focusing on their thermal and rheological properties, as well as their performance in 3D printing. Thermoplastic protein-based composites were prepared through a process of protein dissolution, plasticisation, film casting and pelletisation. Calcium caseinate (CaCas) showed good miscibility with glycerol, whereas phase separation was observed in zein-based composites at higher glycerol concentrations. Zein-based pellets were successfully 3D-printed by using a pellet extruder as the printhead. Stable 3D structures were printed without need for post-processing and an unsupported 45 degrees overhang angle was achieved. Calcium caseinate-based pellets were not extrudable using the current setup due to their high viscosity. Furthermore, the thermal and rheological properties of protein composites correlated well with their printing performance. This study showed that protein-based composites are promising materials for FDM applications.
查看更多>>摘要：This study investigated the effect of enhanced 254 nm ultraviolet-C (UV-C) light exposure on the inactivation of Enterococcus faecium within wheat flour. The in vitro susceptibility of E. faecium on agar plates to UV-C light was determined before performing food studies. The results show significant reduction capacity, achieving an 8.7 +/- 0.2 log reduction after 2.48 J/cm2 of exposure. To determine the effect of enhanced UV exposure on wheat flour decontamination, four redistribution methods were compared based on their inactivation capacity potential. This included no redistribution, manual redistribution, and vibrating continuous redistribution on a novel system using two different sample containers: a Petri plate and a weighing boat. The most effective redistribution method was the continuous redistribution system with a weighing boat base, achieving a 4.1 log reduction in wheat flour after 7.43 J/cm2 of exposure. The reduction capacity was further improved through sample size minimization. Reducing the treatment sample size from 1.00 g to 0.15 g minimized layering and improved the E. faecium reduction in wheat flour from 2.0 log in the 1.00 g sample to 3.6 log in the 0.15 g samples after treatment with a dosage of 2.48 J/cm2. Theoretical adjusted fluence calculations support the need for powdered sample minimization for increased UV exposure, estimating that the 0.15 g wheat flour sample received a dosage approximately 6.66 times greater than the 1.00 g sample. Further optimization of exposure methods through system industrialization could improve on this study's results and accelerate its potential incorporation into powdered food processing.
查看更多>>摘要：Sugar fatty acid esters, serving as multifunctional biosurfactants, are extensively utilized in food formulations for emulsification, foam stabilization, and microbial preservation. Enzymatic transesterification using lipases offers distinct advantages over chemical synthesis, including mild reaction conditions and reduced energy consumption. In this study, we engineered a cold-adapted lipase (Lip) through lid domain truncation and mutation, yielding the optimized variant Lip-8 to alleviate substrate channel steric hindrance, coupled with tannic acidmodified magnetic nanoflowers (TA-MNFs) via a one-pot immobilization strategy to create Lip-8/TA-MNFs. This immobilized enzyme demonstrated significantly enhanced catalytic performance, achieving a 12.91-fold activity increase compared to free lipase. Under optimized conditions (40 degrees C, 36 h), the system synthesized fructose laurate esters with 93.07 % conversion efficiency. Structural characterization confirmed the predominant formation of mono- and di-esters, which exhibit dual surfactant and emulsifying properties suitable for foodgrade applications.
NETL
NSTL
Elsevier