首页期刊导航|Journal of Food Science
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Journal of Food Science
Institute of Food Technologists
Journal of Food Science

Institute of Food Technologists

月刊

Journal of Food Science/Journal Journal of Food ScienceSCIISTP
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    Production of Special Fruit Beer With Addition of Cupuassu ( Theobroma grandiflorum ) Pulp and Prolyl Endopeptidase to Improve Volatile Compounds and Physicochemical Parameters

    Ermor Cesar de Sousa LopesNelio Jacinto Manuel UalemaHellen Kempfer PhilippsenCamilo Barroso Teixeira...
    e70916.1-e70916.16页
    查看更多>>摘要:This study assessed the feasibility of incorporating cupuassu pulp and the commercial protease Aspergillus niger prolyl endopeptidase (AN-PEP) in the production of fruit beer. To preliminarily evaluate the impact of the protease on the beer protein profile, one-dimensional SDS-PAGE was employed. The volatile compounds were analyzed using headspace solid-phase microextraction (HS-SPME)–GC–MS, whereas physicochemical parameters were evaluated following the American Society of Brewing Chemists (ASBC) methods. The band profiles from electrophoresis indicated the degradation of high-molecular-weight proteins, such as gluten, in the formulations treated with protease (A1 and A2). In two out of the three formulations, elevated levels of 3-methyl-1-butanol, linalool, and 2-phenylethanol were detected, suggesting that the protease may have played a role in the release of volatile flavor compounds. The beers produced exhibited low alcohol content, ranging from 2.80% to 3.44% (v/v). On the basis of these findings, we conclude that the combination of AN-PEP and cupuassu pulp is a viable approach to producing fruit beer with a predominantly floral, sweet, and whiskey-like aroma profile, showing preliminary evidence of degradation of high-molecular-weight proteins (including potential gluten fractions) by the action of the protease. Practical Applications The results obtained in this study serve as a foundation for the development of new approaches in the artisanal and sustainable production of fruit beer, providing information for the creation of innovative beers with added value, as well as promoting and valorizing local ingredients for the craft beer sector.

    Investigation of the Impact of Ultrasonic Treatment on the Sensitization of Peanut Allergens and Its Potential Mechanisms

    Haowen FangErlian ShuBing NiuQin Chen...
    e70876.1-e70876.15页
    查看更多>>摘要:Peanut-induced allergic reactions are characterized by rapid onset, high morbidity, and mortality. The current stage to reduce the allergenicity of peanut protein involves various processing methods, including ultrasonication, which is widely used in food processing to disrupt protein structure. In this study, peanut crude protein was treated with ultrasonication and fed to mice. Results showed a significant reduction and alleviated tissue damage in the intestine, lung, and spleen. Additionally, inflammatory factors such as TSLP, IL-33, and so forth were significantly reduced in mice. Furthermore, ultrasound-purified Ara h 2 protein treatment in cells showed a significant reduction in cellular inflammation through the MAPK and NF- κB pathways. This study also found that ultrasonication altered the structure of the Ara h 2 protein, which may be the main reason for its reduced sensitization.

    Sensory Characterization of Cookies With Sugar Replacement by Sweeteners Using the Check-All-That-Apply (CATA) Method

    Igor Henrique Oliveira de LimaNathalia Leticia Hernandez BritoFlavia Aparecida Reitz CardosoRenata Hernandez Barros Fuchs...
    e70888.1-e70888.10页
    查看更多>>摘要:This study investigated the sensory effects of replacing sucrose with blends of xylitol, monk fruit extract ( Siraitia grosvenorii , rich in mogrosides), and thaumatin in 16 prototype cookie formulations. A consumer-based check-all-that-apply (CATA) test ( n = 62 regular cookie consumers) was conducted, followed by Cochran’s Q test, principal component analysis (PCA), and heatmap visualization of attribute citation frequencies. Balanced sweetener systems were defined as formulations that combined an adequate bulk contribution (xylitol) with high-intensity sweeteners (monk fruit and thaumatin) at levels that ensured sweetness equivalence while minimizing off-flavors and structural defects, whereas unbalanced systems lacked this functional complementarity. Twenty-one sensory attributes were evaluated to characterize differences in appearance, texture, and flavor. Significant differences (Cochran’s Q , p < 0.05) were observed among formulations. Cookies containing unbalanced sweetener ratios exhibited high frequencies of crumbly, sandy, floury, pale, and unpleasant sensory attributes. In contrast, balanced combinations, particularly formulation F16 (xylitol 6 g, monk fruit 0.04 g, and thaumatin 0.03 g per formulation), were characterized by soft, moist, tasty, buttery, and crispy attributes with minimal defects. PCA explained 57.9% of the total variance and revealed three sensory archetypes: underdeveloped, balanced, and overbrowned cookies. The results demonstrate that sugar replacement in cookies depends not only on the intensity of sweetness but also on the mechanistic balance between bulk, browning potential, and flavor modulation provided by blended sweetener systems.

    Complexation of Anthocyanin-Rich Blackberry Extract With Ozonated Sodium Alginate: Structural, Rheological, and Color Insights

    Camila YamashitaIzabel Cristina Freitas MoraesCharles Windson Isidoro HaminiukCiro Cesar Zanini Branco...
    e70869.1-e70869.12页
    查看更多>>摘要:Anthocyanins, valued for their health-promoting properties and their role as natural pigments, are highly susceptible to degradation, highlighting the need for strategies to improve their stability. Biopolymer-based systems are an alternative for interacting with and safeguarding these bioactive compounds. Ozonated alginate is a potential candidate for complexing with anthocyanins because of its modified structure and properties. This study aimed to evaluate the complexation of anthocyanin-rich blackberry extract with ozonated sodium alginate (SA) solutions and to compare its properties to those of native (i.e., extracted from seaweed in the lab) and commercial SA. Rheological analyses revealed shear-thinning behavior in the SA and anthocyanin- rich extract composite solutions, indicative of their incorporation and molecular entanglement. Structural analyses provided insights into the electrostatic interaction between the biopolymer and the extract, with new peaks and chemical shifts appearing. Microscopy revealed a porous network, suggesting the formation of complexes between SA and the anthocyanin-rich extract composite solutions. Additionally, the SA and anthocyanin-rich extract solutions exhibited an attractive color. In conclusion, the complex comprising ozonated SA and anthocyanin-rich extract has potential applications in diverse sectors, particularly in the food and medical industries, because it leverages the low molecular weight of SA and the bioactive properties of anthocyanins.

    Nitrogen-Doped Carbon Quantum Dots for Tannic Acid Analysis in Complex Food Matrices

    Qingqing ZouXin WangShan MouPei Zhang...
    e70792.1-e70792.13页
    查看更多>>摘要:The rapid, sensitive, and cost-effective detection of tannic acid (TA) in complex food matrices is essential for improving food safety standards. To meet this demand, nitrogen-doped carbon quantum dots (N-CDs) were prepared using a one-pot hydrothermal reaction using L-malic acid, citric acid, and tris(2-aminoethyl) amine as precursors. The excitation wavelength of the N-CDs was 430 nm, and the emission wavelength was 490 nm. Transmission electron microscopy (TEM), atomic force microscopy (AFM), X- ray photoelectron spectroscopy (XPS), Fourier transform infrared (FT-IR) spectroscopy, and X-ray diffraction (XRD) were used to characterize the prepared N-CDs, and the results revealed a uniform morphology and a nitrogen-rich surface structure. In addition, we found that the N-CDs exhibited excellent selectivity for TA, primarily attributed to the fluorescence quenching effect of TA on the N-CDs. Therefore, a fluorescent sensor for TA detection was constructed based on the N-CDs, with a linear detection range of 0.500-250 μM ( R~2 = 0.99987) and a detection limit of 0.15 μM ( S/N = 3). The practical applicability of the sensor was demonstrated by analyzing TA in beer, red wine, and apple juice, achieving recoveries ranging from 101% to 116% and RSDs between 3.0% and 5.4%. The fluorescence assay results were closely related to the HPLC-DAD validation results, highlighting the accuracy of the method. The N-CDs-based detection platform offers the advantages of simplicity, sensitivity, and reliability, providing a promising tool for routine TA monitoring in food safety applications.

    Encapsulation of Pediococcus acidilactici in Sodium Alginate-Calcium Lactate-Skim Milk Gel Beads: Characterization and Simulated Digestion Analysis

    Ruoxi BaiZhenjia ZhengZhichang QiuWenqing Zhu...
    e70914.1-e70914.16页
    查看更多>>摘要:This study aimed to develop a composite hydrogel matrix of sodium alginate (SA) and skim milk for encapsulating Pediococcus acidilactici CCFM18 and exploring the synergy of these components on protecting the activity and stability of P. acidilactici CCFM18. The synthesis process was optimized using response surface methodology, yielding optimal conditions: calcium lactate (CL) concentration of 4.18% (w/v), curing time of 47 min, and curing temperature of 41℃. The resulting gel beads achieved an encapsulation efficiency of 72.14%, featuring a spherical structure with abundant internal voids. The formation of SA-CL-skim milk gel beads involved cross-linking and hydrogen bonding between the carboxyl groups of SA, Ca~(2+) , and the carboxyl groups/hydroxyl groups of proteins, significantly modifying functional group distributions, x-ray diffraction patterns, rheological behavior, and thermodynamic properties. In vitro simulated digestion analysis revealed that the gel beads effectively protected bacteria from gastric acidity and successfully delivered them to the intestinal environment, achieving a release rate of 84.19% with a viable cell count of 4.9 ×10~7 CFU, which was 8-fold higher than that of unencapsulated bacteria. These findings highlighted the potential of SA-CL-skim milk gel beads in protecting P. acidilactici activity and achieving targeted release.

    A Novel Strategy in Managing Spent Probiotic Culture Media: Metabolome Identified Functional Candidates Against Hyperuricemia

    Hongyu ZhangDa WangXiangheng CaoBaiyu Zheng...
    e70897.1-e70897.15页
    查看更多>>摘要:With the rapid expansion of the probiotics industry, the management of spent culture media has emerged as a pressing concern. However, it also represents a potential resource for functional food development. In this study, we investigated and screened anti-hyperuricemia (HUA) metabolites of Lactobacillus paracasei (LP) from spent media (META). Administration of META significantly reduced serum uric acid (UA) (SUA) levels ( p < 0.05), improved kidney function, and alleviated inflammatory infiltration. Furthermore, META modulated UA metabolism by regulating key enzymes (xanthine oxidase [XOD] and adenosine deaminase [ADA]) and urate transporters (organic anion transporter 3 [OAT3], glucose transporter 9 [GLUT9], and urate transporter 1 [URAT1]), while mitigating HUA-induced inflammation through the Toll-like receptor 4 (TLR4)/nuclear factor kappa-B (NF- κB) pathway. To identify bioactive compounds, untargeted metabolomics based on UHPLC-QTOF-MS/MS detected 361 upregulated metabolites. Network pharmacology and molecular docking (MD) analyses further highlighted potential candidates, including 2-isopropylmaleate and ricinoleic acid methyl ester. Collectively, these findings suggest that probiotic culture media waste may serve as a promising natural source for the development of anti-HUA functional foods. Further studies are warranted to assess the bioavailability and efficacy of these metabolites both in vitro and in vivo.

    Modulation of DBS/Zein/Pectin Nanoparticle Properties by Calcium Ions: Physicochemical and Emulsifying Insights

    Wenhui LiRongrong ZhangJinchi JiangJie Cui...
    e70887.1-e70887.11页
    查看更多>>摘要:This study investigated the effects of Ca~(2+) ions on the physicochemical properties of DBS/zein/pectin (DZP) ternary composite nanoparticles by adding varying concentrations of Ca~(2+) during particle formation. Ca~(2+) ions reduced electrostatic repulsion between colloidal particles, causing collisions and aggregation, which increased particle size. Additionally, Ca~(2+) ions decreased α-helices and increased β-sheets in the zein molecular chains, weakening interactions between zein and other components, and reducing the thermal stability of the composite particles. At high Ca~(2+) concentrations, phase separation occurred, with the crystalline structure nearly disappearing. Ca~(2+) ions neutralized surface charges, decreased colloidal particle wettability, and reduced emulsifying capacity. Emulsions prepared with these particles showed that Ca~(2+) addition intensified the aggregation of composite particles on the emulsion droplet surface, resulting in pronounced phase separation and reduced emulsion stability. Overall, these results demonstrate that Ca~(2+) plays a key regulatory role in governing the structure–property relationships of DZP ternary composite nanoparticles. This study provides mechanistic insight into ion-mediated regulation of protein–polysaccharide systems and highlights the potential of DZP nanoparticles as calcium-responsive carriers for food-related delivery applications.

    Application of Chitosan/CMC Composite Films for Improving the Safety and Quality of Chilled Litopenaeus vannamei

    Nurul Ulfah KarimMohd Amran Aaqillah-AmrRaihanasywa SatyaraniMutiara Alya Wardani...
    e70911.1-e70911.10页
    查看更多>>摘要:Antimicrobial packaging films are widely used to maintain food quality and extend shelf life. This study evaluated the physicochemical and antimicrobial properties of chitosan/carboxymethylcellulose (CMC) composite films and their effectiveness in preserving Litopenaeus vannamei during chilled storage. Chitosan was incorporated at 0%, 1%, 2%, and 4% into CMC films, which were characterized by Fourier transform infrared spectroscopy (FTIR) spectroscopy, revealing maintained structural integrity in all films with –OH/–NH stretching, Amide I and Ⅱ vibrations, and C–O–C ether linkages indicative of strong hydrogen bonding and polymer interactions. The 1% chitosan/CMC film exhibited optimal physicochemical properties, including low moisture content (22.21% ± 4.33%), controlled thickness (0.12 ± 0.02 mm), reduced water solubility (28.17% ± 8.82%), high elongation at break (466.50% ± 8.50%), comparable tensile strength (0.03 ± 0.01 MPa), low opacity (0.01 ± 0.00), and minimal color difference (0.57 ± 0.06). All films demonstrated antimicrobial activity, with larger inhibition zones against Sphingomonas paucimobilis and Staphylococcus schleiferi . Notably, 4% chitosan/CMC film effectively reduced total bacterial and coliform counts (5.77 ± 0.07 and 3.44 ± 0.42 log_(10) CFU g~(-1), respectively) during storage, maintaining shrimp freshness for up to 15 days. These results indicate that 1% chitosan/CMC films are optimal for physicochemical performance, whereas 4% films are preferred for antimicrobial protection. Future improvements to the 1% formulation could include incorporating nanofillers to enhance microbial control without compromising flexibility or transparency, making it more suitable for active seafood packaging. Practical Applications This study demonstrates that chitosan/CMC composite films, particularly those with 1% and 4% chitosan, can be used as eco- friendly packaging or coating materials to help maintain shrimp freshness during storage. The films improve physical stability and provide antimicrobial protection, which may help slow bacterial growth and extend product quality in chilled seafood. These findings offer a potential low-cost, biodegradable alternative for seafood processors seeking safer and more sustainable packaging options.

    Effects of Edible Fungi and Medicine–Food Homology Ingredients on Sensory Attributes and Emotional Responses to Chicken Soup

    Menglin ChengBinyu WangHuayi SuoZhen Wang...
    e70900.1-e70900.15页
    查看更多>>摘要:This study investigated the impact of two types of ingredients, edible fungi ( Lentinula edodes , Agrocybe aegerita , Agaricus blazei Murill , and Dictyophora indusiate ) and medicine and food homology (MFH) substances ( Panax ginseng , Angelica sinensis , and Astragalus membranaceus ) on the sensory attributes and emotional responses elicited by chicken soups. Eight chicken soup samples were evaluated by 12 trained panelists using descriptive analysis (DA) and by 104 consumers using the check-all-that- apply (CATA) method. The results showed that, in addition to significant differences in characteristic ingredient flavors, the intensity of other sensory attributes also differed significantly among the samples. Soups containing the three Agaricales fungi ( Lentinula edodes , Agrocybe aegerita , and Agaricus blazei Murill ) were more strongly associated with fullness, umami, and saltiness. In contrast, the soup prepared with Phallales ( Dictyophora indusiate ) exhibited a distinct flavor profile compared to the other edible fungi. Soups with MFH ingredients were more frequently associated with sweet or bitter flavors. While the overall trends between DA and CATA were consistent, many consumers were unable to accurately identify the characteristic flavors of the added ingredients. CATA emotional response data showed significant differences among the 29 emotional attributes. Among the samples, the soup containing Lentinula edodes received the highest consumer acceptance and was associated with positive emotions such as eagerness, pleasantness, and joy. In conclusion, the addition of different ingredients significantly affects the sensory and emotional attributes of chicken soup. These findings offer new ideas for chicken soup product development and consumer insights. Practical Applications This study formulates a variety of edible fungi or medicine and food homology (MFH) chicken soup combinations to enhance consumer choice and increase sales potential. Furthermore, it serves as a channel to convey consumer feedback to chefs and producers, enabling them to tailor product development to market preferences and create more consumer-led offerings.