期刊,当代医学科学(英文) 2021年卷2期_国家学术搜索

Rapid Establishment of Tracheal Stenosis in Pigs Using Endotracheal Tube Cuff Overpressure and Electrocautery

Jin Hyoung KimJong Joon AhnYangjin JegaSoohyun Bae...

329-335页

查看更多>>摘要：To apply a new airway treatment to humans,preclinical studies in an appropriate animal model is needed.Canine,porcine and leporine tracheas have been employed as animal airway stenosis models using various methods such as chemical caustic agents,laser,and electrocautery.However,existing models take a long time to develop (3-8 weeks) and the mechanism of stenosis is different from that in humans.The aim of the present study was to establish a new and fast tracheal stenosis model in pigs using a combination of cuff overpressure intubation (COI) and electrocautery.Fourteen pigs were divided into three groups: tracheal cautery (TC) group (n=3),COI group (n=3),and COI-TC combination group (n=8).Cuff overpressure (200/400/500 mmHg) was applied using a 9-mm endotracheal tube.Tracheal cautery (40/60 watts) was performed using a rigid bronchoscopic electrocoagulator.After intervention,the pigs were observed for 3 weeks and bronchoscopy was performed every 7 days.When the cross-sectional area decreased by ＞ 50％,it was confirmed that tracheal stenosis was established.The time for tracheal stenosis was 14 days in the TC group and 7 days in the COI-TC combination group.In the COI group,no stenosis occurred.In the COI-TC group,electrocautery (40 watts) immediately after intubation for ＞ 1 h with a cuff pressure of 200 mmHg or more resulted in sufficient tracheal stenosis within 7 days.Moreover,the degree of tracheal stenosis increased in proportion to the cuff pressure and tracheal intubation time.The combined use of cuff overpressure and electrocautery helped to establish tracheal stenosis in pigs rapidly.

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A Participant-assigned Interventional Research of Precesarean Internal Iliac Artery Balloon Catheterization for Managing Intraoperative Hemorrhage of Placenta Previa and Placenta Accreta Spectrum Disorders After Cesarean Section

Yao FANXun GONGNan WANGKe-tao MU...

336-341页

查看更多>>摘要：Placenta accreta spectrum disorder (PASD) and placenta previa (PP) are two of the most hideous obstetric complications which are usually associated with a history of cesarean section (CS).Moreover,women with PASD,PP and/or a cesarean scarred uterus are more likely to have adverse pregnancy outcomes,including blood transfusion,hysterectomy,pelvic organs damage,postpartum hemorrhage,disseminated intravascular coagulation,multi-organ dysfunction syndrome and even maternal or fetal death.This study aimed to investigate the efficacy of precesarean internal iliac artery balloon catheterization (BC) for managing severe hemorrhage caused by PASD and PP with a history of CS.This participant-assigned interventional study was conducted in Tongji Hospital.We recruited 128 women with suspected PASD,PP and a history of CS.Women in the BC group accepted precesarean BC of bilateral internal iliac arteries before the scheduled cesarean delivery.Women in the control group underwent a conventional cesarean delivery.Intraoperative hemorrhage,transfusion volume,radiation dose,exposure time,complications and neonatal outcomes were discussed.There were significant differences in calculated blood loss (CBL) between BC group and control group (1015.0±144.9 vs.1467.0±171.0 mL,P=0.04).Precesarean BC could reduce intraoperative red blood cell (RBC) transfusion as compared with control group (799.5±136.1 vs.1286.0±161.6 mL,P=0.02) and lessen the rate of using blood products (57.1％ vs.76.4％,P=0.02).The incidence of hysterectomy was also lower in BC group than in control group.Postpartum outcomes showed no significant differences between the two groups,except that postoperation hospitalization was longer in BC group than in control group (6.7±0.4 vs.5.8±0.2 days,P=0.03).Precesarean BC of internal iliac artery is an effective method for managing severe hemorrhage caused by PASD and PP with a cesarean scarred uterus,as it could reduce intraoperative blood loss,lessen intraoperative RBC transfusions and potentially decrease hysterectomies.

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NSTL
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维普

A Participant-assigned Interventional Research of Precesarean Internal Iliac Artery Balloon Catheterization for Managing Intraoperative Hemorrhage of Placenta Previa and Placenta Accreta Spectrum Disorders After Cesarean Section

Yao FANXun GONGNan WANGKe-tao MU...

336-341页

查看更多>>摘要：Placenta accreta spectrum disorder (PASD) and placenta previa (PP) are two of the most hideous obstetric complications which are usually associated with a history of cesarean section (CS).Moreover,women with PASD,PP and/or a cesarean scarred uterus are more likely to have adverse pregnancy outcomes,including blood transfusion,hysterectomy,pelvic organs damage,postpartum hemorrhage,disseminated intravascular coagulation,multi-organ dysfunction syndrome and even maternal or fetal death.This study aimed to investigate the efficacy of precesarean internal iliac artery balloon catheterization (BC) for managing severe hemorrhage caused by PASD and PP with a history of CS.This participant-assigned interventional study was conducted in Tongji Hospital.We recruited 128 women with suspected PASD,PP and a history of CS.Women in the BC group accepted precesarean BC of bilateral internal iliac arteries before the scheduled cesarean delivery.Women in the control group underwent a conventional cesarean delivery.Intraoperative hemorrhage,transfusion volume,radiation dose,exposure time,complications and neonatal outcomes were discussed.There were significant differences in calculated blood loss (CBL) between BC group and control group (1015.0±144.9 vs.1467.0±171.0 mL,P=0.04).Precesarean BC could reduce intraoperative red blood cell (RBC) transfusion as compared with control group (799.5±136.1 vs.1286.0±161.6 mL,P=0.02) and lessen the rate of using blood products (57.1％ vs.76.4％,P=0.02).The incidence of hysterectomy was also lower in BC group than in control group.Postpartum outcomes showed no significant differences between the two groups,except that postoperation hospitalization was longer in BC group than in control group (6.7±0.4 vs.5.8±0.2 days,P=0.03).Precesarean BC of internal iliac artery is an effective method for managing severe hemorrhage caused by PASD and PP with a cesarean scarred uterus,as it could reduce intraoperative blood loss,lessen intraoperative RBC transfusions and potentially decrease hysterectomies.

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Clinicopathologic Characteristics and Survival of Patients with Rare Malignant Ovarian Yolk Sac Tumors: A Population-based Analysis

Tian WANGBo WANGShi-xuan WANGMing-fu WU...

342-347页

查看更多>>摘要：Yolk sac tumors (YSTs) are rare malignant germ cell tumors that usually affect young females.To date,there have been few studies on YSTs.We evaluated the relationship between clinicopathologic characteristics of patients with ovarian YSTs and disease outcome based on Surveillance,Epidemiology,and End Results data.The Kaplan-Meier method and log-rank test were used to evaluate differences in survival rates.Data for 269 patients were analyzed.The incidence of YSTs among ovarian germ cell tumors (OGCTs) cases was 0.4％;median patient age was 22.0 years,and most tumors were unilateral.Patients presented with distant metastasis (37.5％),localized disease (49.1％),and regional spread (8.9％).American Joint Committee on Cancer stage was available for 13 patients (stage ⅠA,n=2;stage ⅠC,n=l;stage ⅢA,n=l;stage ⅢB,n=3;stage ⅢC,n=2;and stage Ⅳ,n=4).Survival rates at 1,3,and 5 years were 91.0％,84.0％,and 83.2％,respectively,for overall survival (OS) and 92.0％,85.4％,and 84.5％,respectively,for diseasespecific survival (DSS).The 5-year OS and DSS of patients with ovary tumors were 91.5％ and 92.9％,respectively,compared to 74.8％ and 77.2％,respectively,for those with extra-ovarian spread (P＜0.001 for both OS and DSS).Age ＞50 years was associated with shorter OS and DSS (both P＜0.001),whereas no associatios of OS and DSS were observed with pathologic grade (P=0.49 for OS and 0.52 for DSS).In summary,YSTs are typically unilateral,of a high grade,and localized to the ovary;extra-ovarian spread has a poor outcome,and postmenopausal women have worse prognosis than premenopausal women.

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Clinicopathologic Characteristics and Survival of Patients with Rare Malignant Ovarian Yolk Sac Tumors: A Population-based Analysis

Tian WANGBo WANGShi-xuan WANGMing-fu WU...

342-347页

查看更多>>摘要：Yolk sac tumors (YSTs) are rare malignant germ cell tumors that usually affect young females.To date,there have been few studies on YSTs.We evaluated the relationship between clinicopathologic characteristics of patients with ovarian YSTs and disease outcome based on Surveillance,Epidemiology,and End Results data.The Kaplan-Meier method and log-rank test were used to evaluate differences in survival rates.Data for 269 patients were analyzed.The incidence of YSTs among ovarian germ cell tumors (OGCTs) cases was 0.4％;median patient age was 22.0 years,and most tumors were unilateral.Patients presented with distant metastasis (37.5％),localized disease (49.1％),and regional spread (8.9％).American Joint Committee on Cancer stage was available for 13 patients (stage ⅠA,n=2;stage ⅠC,n=l;stage ⅢA,n=l;stage ⅢB,n=3;stage ⅢC,n=2;and stage Ⅳ,n=4).Survival rates at 1,3,and 5 years were 91.0％,84.0％,and 83.2％,respectively,for overall survival (OS) and 92.0％,85.4％,and 84.5％,respectively,for diseasespecific survival (DSS).The 5-year OS and DSS of patients with ovary tumors were 91.5％ and 92.9％,respectively,compared to 74.8％ and 77.2％,respectively,for those with extra-ovarian spread (P＜0.001 for both OS and DSS).Age ＞50 years was associated with shorter OS and DSS (both P＜0.001),whereas no associatios of OS and DSS were observed with pathologic grade (P=0.49 for OS and 0.52 for DSS).In summary,YSTs are typically unilateral,of a high grade,and localized to the ovary;extra-ovarian spread has a poor outcome,and postmenopausal women have worse prognosis than premenopausal women.

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Association of Plasma Connective Tissue Growth Factor Levels with Hyperthyroid Heart Disease

Huan LIRen-li ZENGYun-fei LIAOMeng-fei FU...

348-355页

查看更多>>摘要：Hyperthyroid heart disease (HHD) is one of the most severe complications of overt hyperthyroidism and increases the risk of mortality in affected patients.Early identification of patients at a higher risk of developing HHD can improve clinical outcomes through active surveillance and management.Connective tissue growth factor (CTGF),a secreted extracellular protein,plays a significant role in cardiac remodeling and dysfunction.We aimed to investigate the association between plasma CTGF level and the risk of HHD in this study.A total of 142 overt hyperthyroid patients without HHD and 99 patients with HHD were included.The plasma CTGF levels were measured using ELISA kits.Routine clinical medical data and echocardiography parameters were recorded for analysis.The plasma CTGF level was significantly higher in patients with HHD than in those without HHD (P=0.002).The plasma CTGF level was positively correlated with free triiodothyronin,tryrotropin receptor antibody,troponin Ⅰ and lactate dehydrogenase levels and the left atrium diameters,right atrium diameters,and right ventricular end-diastolic diameters (all P＜0.05).Logistic regression analysis showed that quartiles 3 and 4 of plasma CTGF levels were significantly associated with the increased risk of HHD (crude OR: 2.529;95％ CI: 1.188-5.387).However,after adjustment for the potentially confounding variables,quartile 4 alone was significantly associated with the higher risk of HHD relative to quartile 1.Hyperthyroid patients with HHD display higher plasma CTGF levels.Furthermore,CTGF is an independent risk factor for HHD.Therefore,the plasma CTGF level may be a potential biomarker for the risk of HHD.

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NETL
NSTL
万方数据
维普

Association of Plasma Connective Tissue Growth Factor Levels with Hyperthyroid Heart Disease

Huan LIRen-li ZENGYun-fei LIAOMeng-fei FU...

348-355页

查看更多>>摘要：Hyperthyroid heart disease (HHD) is one of the most severe complications of overt hyperthyroidism and increases the risk of mortality in affected patients.Early identification of patients at a higher risk of developing HHD can improve clinical outcomes through active surveillance and management.Connective tissue growth factor (CTGF),a secreted extracellular protein,plays a significant role in cardiac remodeling and dysfunction.We aimed to investigate the association between plasma CTGF level and the risk of HHD in this study.A total of 142 overt hyperthyroid patients without HHD and 99 patients with HHD were included.The plasma CTGF levels were measured using ELISA kits.Routine clinical medical data and echocardiography parameters were recorded for analysis.The plasma CTGF level was significantly higher in patients with HHD than in those without HHD (P=0.002).The plasma CTGF level was positively correlated with free triiodothyronin,tryrotropin receptor antibody,troponin Ⅰ and lactate dehydrogenase levels and the left atrium diameters,right atrium diameters,and right ventricular end-diastolic diameters (all P＜0.05).Logistic regression analysis showed that quartiles 3 and 4 of plasma CTGF levels were significantly associated with the increased risk of HHD (crude OR: 2.529;95％ CI: 1.188-5.387).However,after adjustment for the potentially confounding variables,quartile 4 alone was significantly associated with the higher risk of HHD relative to quartile 1.Hyperthyroid patients with HHD display higher plasma CTGF levels.Furthermore,CTGF is an independent risk factor for HHD.Therefore,the plasma CTGF level may be a potential biomarker for the risk of HHD.

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Identification of Dysregulated microRNAs in Glioma Using RNA-sequencing

Chang LIUYing-ying GEXiao-xun XIEBin LUO...

356-367页

查看更多>>摘要：Glioma is the most common malignant brain tumor in central nervous system.Despite advances in the treatment of glioma such as surgery and chemoradiotherapy,most patients are easy to relapse,resulting in adverse clinical outcomes.Hence,effective molecular-targeting treatment may be one of attractive strategies for glioma therapy.The dysregulated microRNAs (miRNAs),one of the candidates of therapeutic targets,are believed to play an important role in the progression of glioma.In this study,we aimed to examine the expression profile of miRNAs in glioma and provide a reference for glioma therapy.Firstly,expression profile of miRNAs in 5 normal brain tissues,5 low-grade glioma (LGG) tissues and 5 glioblastoma (GBM) tissues was detected by RNA sequencing (RNA-seq).Next,the target genes of differentially expressed miRNAs (DEmiRNAs) were predicted and then GO enrichment and KEGG pathway analysis performed by bioinformatics.Finally,10 miRNAs which were significantly up-or down-regulated both in GBM and LGG were validated by real-time quantitative PCR (qRT-PCR).RNA-seq results indicated a number of DEmiRNAs in glioma.There were 64 up-regulated miRNAs and 17 down-regulated miRNAs in LGG,and 181 up-regulated miRNAs and 124 down-regulated miRNAs in GBM,respectively.Bioinformatics analysis showed that the target genes of these DEmiRNAs were enriched in various biological processes and signaling pathways such as cell metabolic and developmental process.Selected DEmiRNAs were further confirmed by qRT-PCR.miRNA-10b-5p,miRNA-92b-3p and miRNA-455-5p were significantly up-regulated in both GBM and LGG;while miRNA-542-3p was significantly up-regulated in LGG;miRNA-184 and miRNA-206 were significantly down-regulated in both GBM and LGG;miRNA-766-5p and miRNA-1-3p were significantly down-regulated in GBM.The subject of our study demonstrated several dysregulated miRNAs may serve as a potential therapeutic target for glioma.

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万方数据

Identification of Dysregulated microRNAs in Glioma Using RNA-sequencing

Chang LIUYing-ying GEXiao-xun XIEBin LUO...

356-367页

查看更多>>摘要：Glioma is the most common malignant brain tumor in central nervous system.Despite advances in the treatment of glioma such as surgery and chemoradiotherapy,most patients are easy to relapse,resulting in adverse clinical outcomes.Hence,effective molecular-targeting treatment may be one of attractive strategies for glioma therapy.The dysregulated microRNAs (miRNAs),one of the candidates of therapeutic targets,are believed to play an important role in the progression of glioma.In this study,we aimed to examine the expression profile of miRNAs in glioma and provide a reference for glioma therapy.Firstly,expression profile of miRNAs in 5 normal brain tissues,5 low-grade glioma (LGG) tissues and 5 glioblastoma (GBM) tissues was detected by RNA sequencing (RNA-seq).Next,the target genes of differentially expressed miRNAs (DEmiRNAs) were predicted and then GO enrichment and KEGG pathway analysis performed by bioinformatics.Finally,10 miRNAs which were significantly up-or down-regulated both in GBM and LGG were validated by real-time quantitative PCR (qRT-PCR).RNA-seq results indicated a number of DEmiRNAs in glioma.There were 64 up-regulated miRNAs and 17 down-regulated miRNAs in LGG,and 181 up-regulated miRNAs and 124 down-regulated miRNAs in GBM,respectively.Bioinformatics analysis showed that the target genes of these DEmiRNAs were enriched in various biological processes and signaling pathways such as cell metabolic and developmental process.Selected DEmiRNAs were further confirmed by qRT-PCR.miRNA-10b-5p,miRNA-92b-3p and miRNA-455-5p were significantly up-regulated in both GBM and LGG;while miRNA-542-3p was significantly up-regulated in LGG;miRNA-184 and miRNA-206 were significantly down-regulated in both GBM and LGG;miRNA-766-5p and miRNA-1-3p were significantly down-regulated in GBM.The subject of our study demonstrated several dysregulated miRNAs may serve as a potential therapeutic target for glioma.

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NETL
NSTL
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Distinguishing Rectal Cancer from Colon Cancer Based on the Support Vector Machine Method and RNA-sequencing Data

Yan ZHANGYuan WUZi-ying GONGHai-dan YE...

368-374页

查看更多>>摘要：Colorectal cancer (CRC) is the third most commonly diagnosed cancer worldwide.Several studies have indicated that rectal cancer is significantly different from colon cancer in terms of treatment,prognosis,and metastasis.Recently,the differential mRNA expression of colon cancer and rectal cancer has received a great deal of attention.The current study aimed to identify significant differences between colon cancer and rectal cancer based on RNA sequencing (RNA-seq) data via support vector machines (SVM).Here,393 CRC samples from the The Cancer Genome Atlas (TCGA) database were investigated,including 298 patients with colon cancer and 95 with rectal cancer.Following the random forest (RF) analysis of the mRNA expression data,96 genes such as HOXB13,PRAC,and BCLAF1 were identified and utilized to build the SVM classification model with the Leave-One-Out Cross-validation (LOOCV) algorithm.In the training (n=196) and the validation cohorts (n=197),the accuracy (82.1％ and 82.2 ％,respectively) and the AUC (0.87 and 0.91,respectively) indicated that the established optimal SVM classification model distinguished colon cancer from rectal cancer reasonably.However,additional experiments are required to validate the predicted gene expression levels and functions.

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