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海南医科大学学报（英文版）
海南医科大学学报（英文版）

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海南医科大学学报（英文版）/Journal Journal of Hainan Medical University
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    SARS-CoV-2 and bioimmunotherapy for ulcerative colitis

    KANG Yin-nanJIE You-chengLIU Shan-shanWANG Jun-ke...
    1-5页
    查看更多>>摘要：Novel coronavirus (SARS‑CoV‑2, SARS‑CoV‑2 for short) infection can cause a series of gastrointestinal damage. Related studies have reported that SARS‑CoV‑2 infection can lead to the occurrence and progression of ulcerative colitis , which may be related to the cytokine storm caused by SARS‑CoV‑2 infection. Recently, we have also paid attention to whether infection with SARS‑CoV‑2 will aggravate the condition of UC patients receiving biological immunotherapy and whether vaccination with SARS‑CoV‑2 is safe and effective for these patients. At present, the interaction mechanism between SARS‑CoV‑2, SARS‑CoV‑2 vaccine and ulcerative colitis is not fully understood, and more research is needed to further clarify the relationship.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    The Hainan papaya extract's effect on anti-aging, learning and memory of aging model mice induced by D-galactose

    XIONG Yun-yunZHOU Yan-ningXIE Chang-caiZHOU Kai...
    6-12页
    查看更多>>摘要：Objective: To explore the effects of Hainan papaya extract on learning and memory impairment and anti‑aging in D‑galactose‑induced aging mice. Methods:A total of 72 Kunming mice with normal cognitive ability screened by water maze test were randomly divided into negative control group, model group, piracetam group, high, medium and low dose groups of Hainan papaya extract (400 mg/kg,200 mg/kg ,100 mg/kg), with 12 mice in each group. Hainan papaya extract and piracetam group were given the above drugs by gavage every day, The negative control and model groups were given the same amount of 0.9％ NaCl solution in the same way. Mice in each group were weighed once a week; At the same time, except for the negative control group, mice in each group were intraperitoneally injected with 2％ D‑galactose every day, and the negative control group was intraperitoneally injected with normal saline for 7 weeks. After 7 weeks, We observed each group of mice's capacity of learning and memory by Morris water maze behavioral test; Then, the content of superoxide dismutase (SOD), malondialdehyde (MDA),catalase (CAT), and nitric oxide synthase ( NOS) were measured; On the other hand, we observed the hippocampus' histopathological changes by hematoxylin‑eosin staining, and measured the protein expression of nuclear factor‑E2‑related factor (Nrf2) in brain tissue of mice in each group by Western blot. Results: After the intervention of Hainan papaya extract on aging model mice, the high, medium and low dose groups could shorten the swimming time and swimming distance of mice to varying degrees, increase the activities of SOD, CAT and NOS in mouse brain tissue and reduce the content of MDA, The performance of high dose group was better than piracetam group ( P<0.01). At the same time, it can improve the histopathological changes of neurons in mouse hippocampus by reducing neuronal nuclear pyknosis,and increase the expression of Nrf2 protein in mouse brain in a dose‑dependent manner. Conclusion: Hainan papaya extract is able to postpone various physical signs of subacute aging mice caused by D‑galactose, and possesses definite anti‑aging and antioxidant effects, which may be related to the regulation of Nrf2 signal pathway.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Effect and Mechanism of Hai Tong-pi Decoction Ointment on Anti-inflammation and Analgesia by Transcutaneous Administration in Mice

    LI Fa-jieGU Jin-yuZHANG-YueSHEN Shuo...
    13-20页
    查看更多>>摘要：Objectve: To investigate the effect and mechanism of Hai Tongpi decoction ointment on analgesic and anti-inflammatory effects in mice after transdermal administration. Methods: The hot plate method and the acetic acid writhing experiment were used to verify the analgesic effect of Hai tong-pi extract on pain model mice; the xylene-induced auricular swelling model and the carrageenan-induced right foot swelling model were used, combined with the ELISA method to determine the analgesic effect. The levels of TNF-α, IL-1β and IL-6 in the serum of mice were observed. Anti-inflammatory effect of Haitongpi Tang decoction ointment on inflammation model mice. Results: In terms of analgesia, compared with the model group, both the treatment group 1 and the treatment group 2 can significantly prolong the hot plate pain threshold and the latency of the writhing response (P<0.05), and significantly reduce the writhing in the mice. The number of times (P<0.001), and the effect of process 1 is more obvious (P<0.05); In terms of anti-inflammatory, compared with the blank group, the swelling degree and swelling rate of the right hind paw of the mice in the model group were significantly increased (P<0.01), indicating that the acute inflammation model was successfully established in the mice. Compared with the model group, both the process group 1 and the process group 2 can significantly reduce the swelling degree and swelling rate of the mice feet (P<0.05), and reduce the auricle swelling degree of the mice, and increase the swelling inhibition rate (P<0.05), and the effective effect of process 1 group was more significant (P<0.05). At the same time, compared with the blank group, the contents of TNF-α, IL-1β and IL-6 in the model group were significantly increased (P<0.01). Compared with the process group 2, the process group 1 had a more significant decrease in the content of IL-1β (P<0.05), and IL-6 content of the two had no statistical difference (P>0.05). Conclusion: The Hai tong-pi decoction ointment prepared by two different processes have good analgesic and anti-inflammatory effects on mice after transdermal administration. inflammatory cytokine expression. The anti-inflammatory and analgesic effect of process group 1 was better than that of process group 2, which may be related to the separate extraction and storage of volatile oil of traditional Chinese medicine, which ensured the long-term pharmacological activity of volatile oil.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Correlation analysis of changes in miR145‑5p /Smads pathway and macrophage polarization in adjuvant arthritis rats

    FAN Wen?jieSHEN XiWAN LeiFAN Hai?xia...
    21-25页
    查看更多>>摘要：Objective: To explore the relationship between the changes of miR145‑5p/Smads pathway and macrophage polarization in adjuvant arthritis rats. Methods: Twelve rats were divided into normal group and model group induced by freund's complete adjuvant (0.1 mL/mouse) by random number table method, with 6 rats in each group. The expression of inflammatory polarization markers IL‑8 and CD206 in synovial tissue was detected by enzyme‑linked immunosorbent assay on the 12th day after the formation of arthritis in rats. Western blotting was used to detect the expression of TGF‑β1/Smads pathway factors in synovial tissues. The expression of miR145‑5P, Smads3 and Smads7 in synovial tissue was detected by RT‑qPCR. Results: Compared with normal group, the expression levels of IL‑8, TGF‑β1 and Smad3 in model group were significantly increased (P<0.05); The expression levels of CD206, Smad7 and miR145‑5P were significantly decreased (P<0.01). The correlation results showed that IL‑8 was positively correlated with Smad3 (P<0.01), IL‑8 was negatively correlated with Smad7 (P<0.05), CD206 was negatively correlated with Smad3 (P<0.01) and positively correlated with Smad7 (P<0.05). miR145‑5p was negatively correlated with Smad3 (P<0.01) and positively correlated with Smad7 (P<0.01). Conclusion: miR145‑5p may inhibit the overactivation of TGF‑β1/Smads pathway, regulate macrophage polarization, and inhibit the development of adjuvant arthritis by inhibiting Smad3 expression.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Efficacy of plasma exchange in adjuvant treatment of chronic and acute liver failure

    LI Chu?yiWANG Jun-keLI BinWEN Xue...
    26-29页
    查看更多>>摘要：Objective: To study and evaluate the clini1cal effect of plasma exchange in the adjuvant treatment of chronic and acute liver failure. Methods:Totally 60 patients with chronic and acute liver failure in our department were divided into two groups. The control group received comprehensive treatment and the plasma exchange group received plasma exchange. The changes of total bilirubin(TBIL) ,PT percentage activity(PTA), alanine aminotransferase(ALT), aspartate aminotransferase(AST), albumin(ALB), interleukin-6(IL-6), procalcitonin(PCT) and C-reactive protein (CRP) were measured after treatment, and were assessed in terms of the degree of relief of clinical symptoms. Results: TBIL, ALB, Alt, AST, IL-6, PCT and CRP in the two groups decreased and PTA increased compared with those before treatment, but the curative effect of the treatment group was better than that of the control group (P<0.05).The total effective rate of the treatment group was 80.0％, which was higher than 46.6％ of the treatment group (P<0.05). Conclusion: Plasma exchange can help to improve the liver function, remove a large number of inflammatory factors, promote the recovery of liver function and improve the prognosis of patients with chronic and acute liver failure.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Meta-analysis for the efficacy and safety of Hanchuan Zupa Granule in treating bronchial asthma

    HAN JingJIA Yan-kunHAN Ze-LluZHAO Rui-heng...
    30-39页
    查看更多>>摘要：Objective: To systematically evaluate the clinical efficacy and safety of Hanchuan Zupa granule(HCZPG) in the treatment of bronchial asthma. Methods: The Cochrane Library, Pubmed, EMBASE, CNKI, CBM, VIP and Wanfang were searched up to June 2021. Randomized controlled trials, which assessed the effects of HCZPG in the treatment of bronchial asthma, were included in this review, and the references included in the selected studies were also tracked. The methodological quality of eligible studies were assessed by the Cochrane Collaboration's tool. Data of the included studies were extracted independently by two authors and meta-analysis was conducted using RevMan5.3. Results: A total of 97 relevant literatures were retrieved, and 13 studies were finally included, involving 1189 patients. The aggregated meta-analysis results indicated that HCZPG improved total clinical efficacy [RR=1.18,95％ CI (1.13,1.24),P<0.00001], FEV1(L)[WMD=0.48,95％ CI (0.22,0.74),P=0.0003], FVC(L)[WMD=1.23,95％ CI (0.5,1.96),P=0.001], FEV1/FVC(％) [WMD=7.37,95％ CI (5.91,8.84),P<0.00001], PEF [SMD=1.19,95％ CI (0.82,1.56),P<0.00001], IL-4 [WMD=-12.13,95％ CI (-16.36,-7.90), P<0.00001], IL-6 [WMD=-29.55,95％ CI (-40.71,-18.39),P<0.00001], TNF-α[SMD=-2.73,95％CI (-3.57,-1.88),P<0.00001],PaO2[WMD=8.97,95％ CI (6.09,11.85),P<0.00001], but the risk of adverse events were not statistically significant between the two groups [RR=0.68,95％CI (0.39,1.18),P=0.17]. Conclusion: HCZPG shows good clinical curative effects in treating bronchial asthma without increasing the incidence of adverse events.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Efficacy of Wendan Decoction series for coronary heart disease with anxiety and depression:A systematic review and meta-analysis

    LI Rui-hanZHANG Jian-zhenHUANG Kai-linJIA Hai-nv...
    40-49页
    查看更多>>摘要：Objective: To systematically evaluate the clinical efficacy of Wendan Decoction series combined with conventional western medicine in the treatment of coronary heart disease complicated with anxiety and depression. Methods: We searched four Chinese databases including CNKI, WanFang, VIP, SinoMed, and five English databases of PubMed, Cochrane Library, Web of Science,EMBASE, Springer Link for clinical randomized controlled trials of using Wendan Decoction series in the treatment of coronary heart disease complicated with anxiety and depression. Time range for searching was from the establishment of these databases to January 2022. Then we screened the retrieved literature according to the inclusion and exclusion criteria,and conducted data extraction and quality evaluation of the selected literature. Meta analysis was conducted using RevMan 5.3 software. Results: A total of 13 RCT studies and 1007 patients with coronary heart disease complicated with anxiety and depression were included. Meta-analysis showed that Wendan Decoction series combined with conventional western medicine treatment showed better effects in the improvement rate of clinical anxiety [RR=1.21, 95％ CI=(1.12,1.31), P<0.00001], the improvement rate of depression [RR=1.30, 95％ CI=(1.10,1.53), P=0.002], the improvement rate of angina pectoris [RR=1.28, 95％ CI=(1.13,1.44), P<0.0001], increasing the ECG improvement rate [RR=1.29, 95％ CI=(1.07, 1.55), P=0.008], increasing the rate of reducing nitroglycerin usage [RR=1.68, 95％ CI=(1.25,2.25, P=0.0006], as well as increasing the improvement rate of TCM symptoms [RR=1.15, 95％ CI=(1.04, 1.26), P=0.004]. It also showed better effects in reducing clinical anxiety and depression scores (SAS, SDS, HAMA, HAMD), SAQ scores and TCM symptom scores. The differences were statistically significant. Conclusion: Wendan Decoction series combined with conventional western medicine shows advantages in treating coronary heart disease patients complicated with anxiety and depression in reducing the angina pectoris score, anxiety and depression score, as well as improving the clinical TCM symptoms.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Research on the Mechanism of the treatment of non‑alcoholic fatty liver by Jianwei Gexia Zhuyu Decoction based on network pharmacology and molecular docking

    YANG JingZHAO Yao?weiNIU JieWANG Rui...
    50-58页
    查看更多>>摘要：Objective: To analyse the key compounds, targets and pathways of the treatment of non‑alcoholic fatty liver disease (NAFLD) by Jianwei Gexia Zhuyu Decoction based on network pharmacology, in order to explore the molecular mechanism of its therapeutic effects. Methods: The differential genes between sick and normal conditions were screened by GEO‑Datasets, and the heat map and volcano map were drawn. The active compounds in Jianwei Gexia Zhuyu Decoction were searched by TCMSP platform and Drugbank database. OB≥30％ and DL≥0.18 were set as thresholds to screen potential active compounds and action targets. The molecular target maps of Jianwei Gexia Zhuyu Decoction and NAFLD differential genes were constructed, and the PPI network and network topology parameters were obtained by STRING database. The PPI network and network topology parameters were visually analyzed by Cytoscape, and the core regulatory genes were screened. At the same time, the SwissDock platform was used to dock the main active components with the target. The main pathways were determined by GO biological function enrichment analysis and KEGG metabolic pathway enrichment analysis by DAVID. Results: After screening, 377 differential genes (127 up‑regulated genes and 250 down‑regulated genes), 225 active compounds of Jianwei Gexia Zhuyu Decoction, 308 corresponding targets were obtained; 14 key targets were screened, corresponding to 168 compounds, and the key targets involved MYC, FOSL2, FOS, etc. The results of GO functional enrichment analysis showed that Jianwei Gexia Zhuyu Decoction mainly regulated the activity expression of DNA binding transcriptional activator and the specific transcription of RNA polymeraseⅡ; The results of molecular docking showed that the main active components quercetin and baicalein had good binding activity with VCAM1, HSPB1, MYC, JUN and so on; The results of KEGG enrichment analysis showed that it was mainly involved in IL‑17 signal pathway, Wnt receptor signal pathway, NF‑κB signal pathway, TNF signal pathway and AGE‑RAGE signal pathway in diabetic complications.Conclusion: Through the interaction of multi‑components and multi‑targets, Jianwei Gexia Zhuyu Decoction has achieved the goal of overall treatment of NAFLD from many ways. The application of network pharmacology provides a new research approach and scientific basis for further study on the mechanism of Jianwei Gexia Zhuyu Decoction in the treatment of NAFLD.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Compatibility rule of traditional Chinese medicine for treatment of cardiac neurosis based on data mining

    LIU XiangWANG ChaoAN YingTANG Zhuo-ran...
    59-66页
    查看更多>>摘要：Objective: To analyze the prescription and medication rule of traditional Chinese medicine(TCM) in treating cardiac neurosis by using the software of "Traditional Chinese Medicine Inheritance Assistant Platform (V2.5)". Methods: China Journal Full-Text Database, Wanfang database, Weipu Chinese Periodical Service Platform and Chinese biomedical literature database were retrieved according to the inclusion and exclusion criteria. Literatures on the treatment of cardiac neurosis with TCM prescriptions were screened out and data mining was carried out using the TCM inheritance assistant platform. Use frequency, commonly used dose, the frequency of properties, flavors and meridian distribution were counted. Association rules and entropy clustering analysis for complex system were used to extract core drug combinations and unsupervised entropy hierarchical clustering was used to predict potential new prescriptions. Results: Through literature retrieval and screening, 210 types of prescriptions were finally selected, and 181 types of Chinese herbs were extracted, with cumulative frequency of 2410 times. Among them, there were 46 traditional Chinese medicines that were used more than 15 times. According to the efficacy and indication, they were classified into qi - regulating drugs, tranquilizers, qi - tonifying drugs, heat - clearing drugs, blood - tonifying drugs, etc. According to properties, flavors, and meridian distribution, warm and sweet drugs were the most commonly used, mostly distributing to the heart meridian, followed by liver and spleen meridians. Association rules showed that combination of radix paeoniae and paeonia-bupleurum was the most used; faructus aurantii and bupleurum had the strongest correlation, up to 90％. The network diagram indicated that bupleurum, angelica sinensis, radix paeoniae radix, and poria cocos were the core of the association network. Based on complex system entropy clustering, 24 core drug combinations were obtained. The unsupervised entropy hierarchical clustering algorithm was used to extract 12 core drug combinations, and 6 potential new formulae were obtained by further clustering. Conclusion: Regulating qi and blood, addressing both symptoms and causes,and using purgation and tonification at the same time is the principle of the treatment of cardiac neuropathy with TCM. The treatment mainly starts from the heart, liver and spleen, and therapies include soothing the liver and regulating qi, clearing heat and resolving phlegm, tranquilizing and calming the nerves,and prompting blood circulation and diuresis.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Advances in molecular mechanism of myocardial ischemia/reperfusion injury

    WEI Cheng-luFENG Qing-minCHEN Song-chengZHUO Huai-mi...
    67-72页
    查看更多>>摘要：Myocardial ischemia/reperfusion (I/R) injury is a pathological change that occurs during the restoration of blood supply to ischemic or occluded coronary arteries after coronary heart disease, stroke, cardiac arrest and resuscitation, organ transplantation, shock and other events. Myocardial I/R injury is often accompanied by cardiovascular adverse events, which seriously affect the prognosis of myocardial ischemia. The potential mechanism of myocardial I/R injury is complex, involving many pathological processes, such as oxygen free radical injury, calcium overload, inflammation, apoptosis, activation, immune imbalance, endoplasmic reticulum stress, autophagy, myocardial energy metabolism disorder, myocardial microvascular endothelial cell injury and so on. Ischemic preconditioning is an effective preventive and therapeutic measure to reduce ischemia-reperfusion (I/R) injury. At present, there are many experimental studies on the pathogenesis and prevention and treatment measures, but the clinical application is still limited, so the prevention and treatment of I/R injury is still a major challenge. The pathogenesis of myocardial I/R injury is not completely clear, and the treatment methods and drugs are limited, so this paper summarizes the molecular mechanism and related signal pathways involved in its injury, as well as the emerging targeted therapy, to provide strategy and theoretical basis for clinical prevention and treatment of myocardial I/R injury.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普