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海南医科大学学报（英文版）
海南医科大学学报（英文版）

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海南医科大学学报（英文版）/Journal Journal of Hainan Medical University
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    The latest research progress in asymptomatic and pre-symptomatic patients with COVID-19

    Hui-Juan ShaoXue-Feng MaJun HuangXiao-Hui Yu...
    1-5页
    查看更多>>摘要：The asymptomatic and pre-symptomatic infections of coronavirus disease 2019 (COVID-19) has no perceptible clinical symptoms and signs, and it is not easy to be detected. The virus' transmission of asymptomatic and pre-symptomatic infections carried is concealed, which brings great challenges to the control of the epidemic. Due to the length of the incubation period, some studies have failed to distinguish between asymptomatic and pre-symptomatic infections, resulting in a higher proportion of asymptomatic infections. This article reviews the latest research progress of characteristics, transmission, detection and control of asymptomatic and pre-symptomatic infection.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Effect of Soufeng Qutan Chinese medicine on blood lipid and IL-1β in atherosclerotic mice with AopE gene knockout

    Bing ZhaoLi-Hong GongFu-Long Xiao
    6-10页
    查看更多>>摘要：Objective: To explore the possible mechanism of Soufeng Qutan traditional Chinese medicine in the treatment of atherosclerosis. Methods: 6-8 weeks old ApoE knockout mice were fed with a high-fat diet for 13 weeks to establish an atherosclerotic unstable plaque model, and they were randomly divided into model groups, low, medium, and high-dose Shoufeng expectorant groups. Torvastatin group; another 6-8 week old C57BL/6 mice were set as blank control group. Both the blank control group and the model group were given 0.9％ normal saline by gavage. The low, medium, and high-dose groups of Soufeng Expectorant Chinese Medicine and the atorvastatin group were given different doses of Soufeng Expectorant Chinese Medicine Decoction and Atorvastatin. intervention. After 13 weeks, the mice were sacrificed, and the abdominal aortic blood vessels were histopathologically observed by HE staining. The blood lipids, total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C) were detected by a biochemical automatic analyzer), high-density lipoprotein cholesterol (HDL-C) content and ELISA method to detect IL-1β levels. Results: Compared with the blank control group, a large number of foam cells can be seen in the model group, and the plaque area is obvious; serum TC, TG, LDL-C, IL-1β expression levels increased (P<0.05), and HDL-C expression levels decreased (P<0.05); Compared with the model group, each dose group of Soufeng expectorant Chinese medicine and atorvastatin group had a small amount of foam cells and reduced plaque area; serum TC, TG, LDL-C, IL-1β expression levels Both decreased (P<0.05), and the expression level of HDL-C increased (P<0.05). Compared with the atorvastatin group, there was no significant difference in the expression levels of TC, TG, LDL-C, HDL-C, and IL-1β in the high-dose Soufeng and expectorant Chinese medicine group (P>0.05). Conclusion: Soufeng Expectorant Herbal Medicine can inhibit the progression of cellular inflammation, down-regulate the expression of IL-1β, reduce plaque lipid content, and improve blood lipids. This may be one of the molecular mechanisms of Soufeng Expectorant Herbal Medicine in the early treatment of atherosclerosis.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Mechanism of Resveratrol on autophagy mediated by Mst1/Sirt3 signaling pathway in diabetic cardiomyopathy

    Zhen-Wang MaDe-You JiangXing-Xing YuanZhen-Yu Li...
    11-16页
    查看更多>>摘要：Objective: To observe the effects of Resveratrol on myocardial cell injury and Mst1/Sirt3 signaling pathway mediated autophagy in type 2 diabetic mice. Methods: The mice were allocated to normal control group, model group, Resveratrol group, and melbine group, with 10 mice per group. Resveratrol group and melbine group were treated with resveratrol and metformin, respectively. The NC and the model group were treated with equal volume of normal saline by gavage for 8 consecutive weeks. HE staining, transmission electron microscopy and immunofluorescence were used to observe the pathological morphology, ultrastructure and apoptosis levels of myocardial tissues. RT-qPCR method was used to detect the expression levels of apoptosis genes Bax and Bcl-2 in myocardial tissues, and Western-blot method was used to detect the expression levels of autophagy proteins (LC3 and p62), Mst1 and Sirt3 proteins in myocardial tissue; Results: Compared with the model group, Resveratrol can significantly reduce the body weight, blood glucose level and serum CK and LDH levels of db/db mice, and the difference is statistically significant (P<0.05 and P<0.01). Meanwhile, after Resveratrol treatment, myocardial inflammation score, apoptosis rate, Bax mRNA expression level and Bax/Bcl-2 ratio in myocardial tissue were significantly reduced, and Bcl-2 mRNA expression level was significantly increased, and the difference was statistically significant (P <0.01). In addition, compared with the model group, the expression level of p62 and p-Mst1 protein in the myocardial tissue of the Resveratrol group was significantly reduced, and the expression level of Sirt3 protein and the ratio of LC3II/LC3I were significantly increased, and the difference was statistically significant (P<0.01). Conclusion: Resveratrol promotes the autophagy level of cardiomyocytes by activating the Mst1/Sirt3 signaling pathway and inhibits cardiomyocyte apoptosis to play a protective role in diabetic cardiomyopathy.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Identification and analysis of core target genes of miR-29b-3p in glioma

    Sheng-Hua ZhuoLiang-Wang YangShen-Bo ChenJin-Ben Zhang...
    17-21页
    查看更多>>摘要：Objective: To investigate the core target genes of miR-29b-3p, and analyze the clinical significance of the core target genes in glioma. Methods: Bioinformatics analysis was used to predict and screen the target genes of miR-29b-3p. STRING and Cytoscape software were used to analyze the protein-protein interaction (PPI) of target genes. the differences expression and survival prognosis in glioma were analyzed by GEPIA and CGGA. Independent prognostic factors analyzed by univariate and multivariate Cox proportional hazards regression model. Results: 22 target genes of miR-29b-3p were predicted using LinkedOmics, miRDB, miRTarBase, TargetScan, and starbase databases. Through the construction of the PPI network, genes out of the network were removed, and a total of 16 genes were screened for further study of their clinical significance. Based on analysis of GEPIA and CGGA databases, COL2A1, DNMT3A, and DNMT3B were excluded. Through further analysis of the univariate and multivariate Cox proportional hazard regression model, finally identified three core target genes: SERPINH1, LOXL2, CDK6. Conclusion: Bioinformatics analysis showed that miR-29b-3p targeted three core genes such as SERPINH1, LOXL2, and CDK6 in glioma. The expression of these genes was different between brain normal tissues and gliomas, between different grades of tumor, IDH mutation status and 1p/19q codeletion status. Its high expression had adverse effects on overall survival and recurrence-free survival. These core target genes can be used as an independent prognostic factor.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Analysis of risk factors and establishment of predictive model for elevated ALT in adult patients with nonalcoholic fatty liver disease

    Shao-Jie DuanZun-Jing LiuJia-Liang ChenShu-Kun Yao...
    22-27页
    查看更多>>摘要：Objective: To explore the risk factors of elevated alanine aminotransferase (ALT) in patients with nonalcoholic fatty liver disease (NAFLD), and to establish a risk prediction model of elevated ALT in patients with NAFLD. Methods: A total of 200 NAFLD subjects were enrolled in a third-class hospital in Beijing. The relevant clinical indexes and TCM tongue picture data were collected standardized. According to the elevation of ALT, the patients were divided into ALT elevation group and ALT normal group. The independent risk factors of ALT elevation were obtained by logistic regression analysis. Based on this, the logistic regression prediction model of ALT elevation in NAFLD patients was established, and the calibration of the model was evaluated by Hosmer-Lemshow goodness-of-fit test. The area under the subject's working characteristic curve (AUROC) was used to test the discrimination of the model. Results: The multivariate logistic regression analysis showed that the OR value of male, obesity, elevated total cholesterol (TC), elevated triglyceride (TG) and prickly tongue were 6.059, 2.216, 2.649, 2.106, 3.646, respectively, and the P-values were all < 0.05. The AUROC of logistic regression prediction Model 1 (without prickly tongue) and Model 2 (include prickly tongue ) were 0.771 (95％CI:0.703-0.840) and 0.801 (95％CI:0.736-0.866), respectively, and the maximum Youden index, sensitivity and specificity were 0.414, 0.829, 0.585 and 0.478, 0.686, 0.792, respectively. Conclusion: Male, obesity, elevated TC, elevated TG and prickly tongue were independent risk factors for elevated ALT in NAFLD patients; This study established an integrated traditional Chinese and Western medicine model that includes the tongue characteristics, which have certain clinical value in predicting the risk of elevated ALT in patients with NAFLD, and are worth popularizing and applying.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Study of Guanhuang Ganmao Keli on the antiviral activity on human respiratory syncytial virus

    Yue WuXu YangTao SunTao Liu...
    28页
    查看更多>>摘要：Objective: To study the antiviral activity of Guanhuang Ganmao Keli on human respiratory syncytial virus (RSV) in vitro. Methods: The Guanhuang Ganmao Keli was dissolved in pure water and filtered by a 0.22 micron filter to get solution. Cyclopiazonic acid (CPA) was used as positive control. The toxicity of Guanhuang Ganmao Keli on Hep-2 cells was tested by cell counting kit 8 (CCK-8). The protective effect of Guanhuang Ganmao Keli on RSV was evaluated under the highest toxic concentration. Results: The TC50 and EC50 of Guanhuang Ganmao Keli is 0.647 mg/ml and 0.014 mg/ml, respectively. Guanhuang Ganmao Keli showed significant antiviral effect when added 0、2、4、6 and 8h post-infection. Conclusions: Guanhuang Ganmao Keli is an effective antiviral agent on RSV in vitro.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    A systematic review and meta-analysis for platelet-rich plasma in treatment of knee osteoarthritis

    Rong XieShang-Quan WangLing-Hui LiXu Wei...
    29-36页
    查看更多>>摘要：Objective: To systematically evaluate the efficacy and safety of platelet-rich plasma in patients with knee osteoarthritis. Methods: The Pubmed, Cochrane Library, Embase,and Medline were investigated from 2005 through May 2020.All randomized double-blind controlled trials were included the effect of PRP injections for KOA. Literature screening and data extraction were carried out independently by two researchers, and the quality of the included randomized controlled trials was evaluated one by one according to the Cochrane collaboration standards. Meta-statistical analysis was performed for the studies that met the inclusion standards using RevMan5.3. Results: In this study,(1)Seven studies met the eligibility criteria, including 250 patients in the experiment (PRP)group and 230patients in the control(no PRP)group.(2)Meta-analysis results showed that the WOMAC Scores was differed significantly between the PRP and no PRP groups at the 6 months follow-up(MD=9.63,95％CI:3.29 to 15.96,P=0.003),and 12 months follow-up(MD=3.85,95％CI:2.66 to 5.04,P<0.00001)(P<0.05).In a comparison of KOOS function scores at the 6 months follow-up, PRP improved knee function scores more than no PRP(MD=8.63,95％CI:0.85 to 16.42,P=0.03) (P<0.05).PRP injections reduced pain more effectively than no PRP injections in patients with KOA at 3,6 months of follow-up, and the VAS pain score showed a significant difference(SMD=0.88,95％CI:0.13 to 1.62,P=0.02) (SMD=0.85,95％CI:0.11 to 1.59,P=0.002)(P<0.05). However, Adverse Events (AEs) were all not significantly different(RR=0.96,95％CI:0.65 to 1.40,P=0.82)(P>0.05).(3)Results showed that PRP has significant advantages in pain relief and functional improvement. Conclusions: In improving knee function and relieving pain, PRP showed superiority to other non-surgical treatments in long-term follow-up from well-designed double-blind trials. Due to the limited quality and data, more high-quality multi-center studies are required.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Effect of synoviectomy combined with postoperative radiotherapy on pigmented villonodular synovitis in knee: A meta-analysis

    Qi-Zhong LaiTian-Ye LinPeng YangBing-Lang Xiong...
    37-44页
    查看更多>>摘要：Objective: Surgical resection of the diseased synovium is the main method for the treatment of pigmented villonodular synovitis in knee. Postoperative radiotherapy is an adjuvant therapy to reduce recurrence. However, whether it should be combined with radiotherapy is controversial. The study is designed to evaluate the efficacy of synovectomy combined with postoperative radiotherapy in the treatment of pigmented villonodular synovitis compared with only synovectomy in knee. Methods: The related references were searched from SinoMed, CNKI, Wanfang, VIP, PubMed, Embase, Cochrane Library and Web of Science from inception to October 2020, and the studies of synovectomy combined with postoperative radiotherapy were screened. According to the type of research, the corresponding evaluation methods were selected for quality evaluation of the literature. RevMan 5.3 software was used for meta-analysis. The primary outcome was recurrence, and the secondary outcome included complications, excellent and good rate of function of knee, range of motion of knee, Lysholm score and IKDC score of knee etcetera. Results: A total of 11 articles were included in meta-analysis, with 510 cases, 280 in the combined group and 230 in the control group. The results of meta-analysis showed that: (1) The recurrence rate in the combination group was significantly lower than that in the control group, OR=0.33 [95％CI (0.20,0.55)], p < 0.0001. (2) The complications in the combined group were higher than that in the control group, OR=2.70 [95％CI (1.16,6.31)], p=0.02. (3) The excellent and good rate of the combined group was significantly better than that of the control group, OR=2.86 [95％CI (1.23,6.64)], p=0.01, and it also may be the result of different evaluation criteria of each study. (4) There was no significant difference range of motion[MD=2.96,95％CI(-0.54,6.47),p=0.10], Lysholm score[MD=3.22,95％CI(-0.51,6.96),p=0.09] and IKDC score[MD=2.88,95％CI(-1.07,6.82),p=0.15] in knee between the two groups. Conclusions: The meta-analysis shows that synovectomy combined with radiotherapy can reduce the recurrence rate of pigmented villonodular synovitis in knee, but compared with only operation, there are higher complications. However, the above conclusions need to be verified by long-term follow-up of more high-quality clinical studies.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Molecular mechanism of treatment of pneumonia in children with Mori cortex - Lycii cortex based on network pharmacology

    Fu-Shuang YangFang CaoYi-Bu KongYan Xu...
    45-52页
    查看更多>>摘要：Objective: To study the molecular mechanism of Mori Cortex - Lycii Cortex for infantile pneumonia based on network pharmacology. Methods: TCMSP and BATMAN-TCM online prediction database were used to screen and collect the active ingredients and targets of Mori Cortex - Lycii Cortex with oral bioavailability (OB) and drug-like(DL). Predictive analysis of disease targets was conducted through PubMed, GeneCards and DrugBank databases. The component-target regulation network was constructed by using Cytoscape 3.7.2 software, and the network topology of the core target was analyzed. Finally, the Bioconductor platform and R language were used for GO function analysis and KEGG pathway enrichment analysis, and the target-key pathway network diagram was constructed. Results: A total of 43 active components, including quercetin, kaempferol, acacetin, and beta-sitosterol, were identified with 242 potential targets. There were 3271 pneumonia targets in children, among which the key targets were IL-6, AKT1, MAPK8, etc. There were 31 common targets of MMP9, TNF, AKT1 and so on. GO biological processes include the response to lipopolysaccharides, molecule of bacterial origin, metal ions, regulation of apoptotic signaling pathway, and T cell activation. The KEGG signaling pathways involved mainly include TNF, PI3K/AKT and MAPK signaling pathway.Conclusion: Mori Cortex - Lycii Cortex for quercetin, kaempferol, beta-sitosterol and acacetin element composition such as possible through the AKT1, MAPK8, IL-6 and MMP9 targets in the role of such as TNF, PI3K/AKT, MAPK signal pathways and so on several signal transduction pathways that play to the role of the treatment of children pneumonia, the results can be for the further study of Mori Cortex - Lycii Cortex for mechanisms to provide the reference for the treatment of pneumonia in children.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Study on prescription and medication rules of Hua Baojin for treatment of colorectal cancer based on data mining

    Meng-Qi ChengYu-Wei ZhaoJu-Ling JiangHong-Gang Zheng...
    53-59页
    查看更多>>摘要：Objective: To study the prescription and medication rule of Professor Hua Baojin in the treatment of colorectal cancer through data mining, so as to provide reference for clinical treatment of colorectal cancer. Methods: The outpatient medical records of Professor Hua Baojin from June 2015 to October 2020 in Guang'anmen Hospital, China Academy of Chinese Medical Sciences were collected. TCM Inheritance Support Platform (V2.5) was used to analyze high-frequency drugs, drug properties, flavors, belonging meridians, common drug combinations, core combinations and new prescriptions. Results: A total of 500 prescriptions were included, involving 222 traditional Chinese medicines and 38 high-frequency (≥ 100) medicines, including Atractylodes, Poria cocos, Ginger, etc. The most common medicinal properties of drugs were warm, cold and mild, flavors were sweet, bitter and pungent, belonging meridians were spleen, stomach, liver and lung meridians. 36 groups of common drug combinations and 20 association rules were obtained by data mining, and 18 core combinations and 9 new prescriptions were evolved. Conclusion: Professor Hua Baojin takes recuperating spleen and stomach as the core in the treatment of colorectal cancer, attaching importance to regulating the rise and fall of qi, adding and subtracting on the basis of Xiangsha Liujunzi Decoction, flexibly selecting the drugs of dispelling blood stasis, resolving phlegm, detoxification and loose knots, and using both cold and warm in the prescription, tonifying and reducing at the same time.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普