查看更多>>摘要：Background Cotton(Gossypium hirsutum L.),adapted to tropical and subtropical regions of the world,is highly sensitive to low temperatures throughout its life cycle.The objective of this study was to evaluate the mitigating effects of different doses of animal-derived(0.25％,0.50％,and 1.00％Isabion®),seaweed-based(0.165％,0.330％,and 0.660％Proton®)biostimulants,as well as a copper(Cu)-containing fungicide application,on cotton cultivar Lazer seedlings at the four true leaves(V4)stage.The plants were exposed to a low temperature of 5 ℃ for 48 h,and the changes in morphological(seedling fresh and dry weight,plant height,and stem diameter)and physiological parameters(leaf temperature,chlorophyll content,relative water content,electrolyte leakage,and relative injury)were examined.Results The results revealed that chilling stress reduced plant growth,while biostimulants helped protect the plants and overcome the adverse effects of chilling.Under chilling stress,there was a considerable reduction in seed-ling fresh weight(SFW),seedling dry weight(SDW),plant height(PH),stem diameter(SD),leaf temperature(LT),and relative water content(RWC).Cotton seedlings treated with the animal-derived biostimulants showed signifi-cantly enhanced SFW,SDW,PH,SD,LT,chlorophyll content(Chi),electrolyte leakage(EL),and relative injury(RI),although there were no positive changes in RWC.No significant differences in the morphological traits were observed among the doses of seaweed biostimulants.For SDW,PH,EL,and RI,the best results were obtained with the applica-tion of a fungicide containing copper.Conclusion These results show the efficiency of the biostimulant and fungicide treatments in mitigating low-temperature stress in cotton seedlings.Applying a copper-containing fungicide to cotton seedlings helped to coun-teract the negative effects of low-temperature stress and to protect the plants from damage by maintaining elec-trolyte balance.Among the biostimulant applications,all levels of animal-derived biostimulant applications,as well as the 0.660％level of the seaweed-derived biostimulant,led to increased tolerance of cotton plants to chilling stress.
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