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中国海洋大学学报（自然科学英文版）
中国海洋大学学报（自然科学英文版）

文圣常（院士）

季刊

1672-5182

xbywb@ouc.edu.cn

0532-82032408

266003

山东省青岛市鱼山路5号

中国海洋大学学报（自然科学英文版）/Journal Journal of Ocean University of ChinaCSCDCSTPCD北大核心SCI
查看更多>>创刊宗旨：我刊及时反映和报道海洋科学与工程、海洋环境科学与工程、海洋气象、海洋药物、海洋遥感、海洋信息系统与管理工程、水产、湖沼等领域的最新研究成果。论文栏目设置：研究论文、技术报告、研究快报、高新技术与信息跟踪、特约评介、综述等。读者对象：国内海洋及相关学科领域的科技人员、教师和管理人员，主要是国际海洋科学界的同行，以英语为语言工具的读者。
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    Effect of Sodium Alginate and Calcium Chloride on the Physicochemical Characteristics of Shrimp(Penaeus Vannamei)Surimi Gels

    WANG ZiyanFENG JiehuiLI QianqianJIA Ru...
    1645-1654页
    查看更多>>摘要：The object of this study is to investigate the effects of sodium alginate(SA)and CaCl2 on the physicochemical characte-ristics of shrimp surimi by studying gel strength,water holding capacity(WHC),as well as by texture profile analysis(TPA).In addi-tion,the mechanism was analyzed through chemical interactions,protein secondary structure,and microstructure.The results showed that with the addition of different concentrations of SA and CaCl2 to the shrimp surimi,the gel quality firstly increased and then de-creased with the increase of SA and CaCl2 concentrations.The highest values of WHC,breaking force and gel strength were obtained with the addition of 1.2%SA or 0.1%CaCl2.When SA and CaCl2 were used in concert,the group containing 1.2%SA and 0.15%CaCl2 had the highest gel strength with the densest three-dimensional network structure of the gel.In addition,the results of chemical interaction analyses showed that hydrogen and ionic bonds were the main chemical bonds of shrimp surimi sol,while shrimp surimi gel mainly consisted of hydrophobic and disulfide bonds.The incorporation of SA and CaCl2 resulted in a significant increase in hydrophobic interactions and disulfide bonding,which could effectively improve the gel properties of shrimp surimi.
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    Effects of Functional Fish Organizations on the Fractal Characteristics of the Microstructure of Feces

    QIAO LinaWANG HongshuaiLIU Yao
    1655-1665页
    查看更多>>摘要：Fish feces affect the structure and function of aquatic ecosystems,and they are affected by the functional fish organiza-tions.In this research,Ctenopharyngodon idellus,Hypophthalmichthys molitrix,and Cyprinus carpio were selected to study the ef-fects of different functional fish organizations on the fractal characteristics of fecal micro-structure by scanning electron microscopes(SEM),particles(pores)and cracks analysis system(PCAS).The results showed that fish feces pores mainly consisted of medium pores(cumulative pore number,97%)classified by the International Union of Pure and Applied Chemistry(IUPAC).The grain area fractal dimension D1 and the pore-number and pore-size fractal dimension D2 were 1.94-1.96 and 2.07-2.19,respectively.The dis-tribution of fish feces pores was very close to the Sierpinski carpet structure,which is the basic fractal construction methods widely used to describe the fractal of pore surface distribution.D1(1.96)and D2(2.19)of Hypophthalmichthys molitrix were the maximum values of the three functional organizations.Combining with the habit of fish,it is inferred that the feces of H.molitrix,the finer the feed and the faster the swimming of fish,the higher the content of feces clay,the larger the fractal dimension of feces,the easier it is to decompose feces,and the high the content of nutrients and organic matter to release into the water.It is demined that fish func-tional organizations affected the fractal characteristics and the stability of fish feces in water.This study is helpful for further research on water quality prediction and the impact of functional fish organizations on the structure and function of the ecosystem.
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    Rapid and Efficient Investigation of Ciliate Nuclear Development During Conjugation Through Opti-mizing Hoechst33342 Staining Workflow

    JIANG YaohanZHANG XueLIU DanTANG Xianglin...
    1666-1676页
    查看更多>>摘要：Ciliates are eukaryotic unicellular organisms with complex morphology and developmental processes,including asexual and sexual processes.Conjugation is a form of sexual process that renews genetic materials.However,visualizing conjugation in ci-liates is a challenge due to the complexity and dynamics of the process,while traditional staining methods are often insufficient for the research.This study introduces a new method for visualizing developmental progression in the nuclei during conjugation using Hoechst33342 staining.It describes how to proceed from cell culture,conjugation induction and synchronization,staining preparation,and observation to statistical analysis.The combination of fluorescent staining with the'volume-fixing'technique eliminates the fixa-tion and dehydration steps,thus reducing the overall operation time to just 20 minutes.This method offers several advantages over tra-ditional staining techniques for studying the nuclei during conjugation.It improves image quality and workflow efficiency and en-ables real-time observation of live cell states.Potential solutions to challenges that may arise during experimental procedures are in-troduced and references and guidelines for cytological research are provided in this paper.
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    Dynamics of Haptophytes(Eukaryota,Hacrobia)Revealed via Short-Term Sampling in the South China Sea

    ZHANG HaoyueLI RanGU BoweiKONG Hejun...
    1677-1688页
    查看更多>>摘要：Haptophytes(Eukaryota,Hacrobia)play a crucial role in the energy budget and element cycling of diverse aquatic eco-systems due to their ability to engage in both phototrophic and mixotrophic nutritional modes.Nevertheless,there is a significant lack of knowledge regarding the short-term variations,such as diel dynamics,of their ecological features.During a short time frame in the summer of 2018,samples were collected from three distinct water layers in the South China Sea,including surface water,the deep chlorophyll maximum(DCM)layer,and 200 m depth.Fluorescence in situ hybridization coupled with tyramide signal amplification was used to quantify haptophyte cell abundance.Most haptophyte communities in all three water layers were composed of cells 2-5 µm in size,while the proportion of cells<2 µm increased with water depth.High-throughput sequencing of the V4 hypervariable regions of the SSU rRNA revealed that Chrysochromulina and Phaeocystis predominated the community,and the former was more abundant in the surface water and 200 m depth and the latter was more abundant in the DCM layer.Higher abundance of small cells(<2 µm and 2-5 µm)during the night was found compared to the day time,whereas large cells(5-10 µm and 10-20 µm)were more prevalent du-ring the day time.The results of correlation analyses showed that haptophyte abundance was possibly impacted by both environmen-tal biotic(heterotrophic nanoflagellates,heterotrophic bacteria,and viruses)and abiotic(temperature,salinity,and nutrients)factors.
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