Sara Maria PaniValentina CoroneoViviana PasquiniAdriana Sanna...
603-610页
查看更多>>摘要：Objective:The overexploitation of the sea urchin Paracentrotus lividus stock,as a consequence of the growing market demand for roe,has boosted the research for echinoderm rearing.The chemical,physical,and microbiological characteristics of land-based facilities are crucial for sea urchins'health and human consumption of their products.In Italy,health-hygienic regulations for P lividus rearing are still to be perfected by the authorities.In this context,we characterized the microbiological quality of a pilot land-based facility for sea urchin production at the University of Cagliari(Italy)to support the development of technical production regulations.Materials and Methods:The accredited Hygiene Laboratory of Cagliari University collected and analyzed the samples in June 2023.Mesophilic bacteria,yeasts,and molds were searched for in air and on surfaces.Total coliforms and Escherichia coli,Enterococci,Pseudomonadaceae,Staphylococcus aureus,sulfite-reducing Clostridia,and Vibrio spp.were identified in water samples.We searched for Vibrio spp.and Pseudomonas spp.in the gonads and coelomic fluid of sea urchins.Results:Although air,surfaces,and water quality were satisfactory overall,some critical points should be monitored more strictly.Enterococci concentration was 250 CFU/100 mL in the water reserve,suggesting animal contamination(other than humans).Pseudomonas aeruginosa was the most resistant to filtration processes,with a residual concentration of 6 CFU/250 mL after the second filtration.No colonies of Vibrio spp.or Pseudomonas spp.were isolated in sea urchins'gonads or coelomic fluid.Conclusions:Starting from the results,we provided targeted advice for developing technical production regulations,system monitoring,and facility routine maintenance in accordance with the'best practice'approach.This analysis could be considered a first step toward the elaboration of common regulations about the minimal standards for the breeding environment of P.lividus by national and regional authorities.
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