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当代医学科学(英文)
当代医学科学(英文)

龚菲力；冯敢生

双月刊

2096-5230

jtmu@tjmu.edu.cn

027-83692514

430030

武汉市航空路13号同济医学院学报

当代医学科学(英文)/Journal Current Medical ScienceSCI
正式出版
收录年代

    Mast Cells Contribute to Pressure Overload-induced Myocardial Hypertrophy by Upregulating TRPV4 via Histamine:Role of Ca2+/CnA/NFATc3 Signaling Pathway

    Zhi-dong ZHANGTing LIANQuan-yi CHENGMei-ping ZHU...
    1071-1080页
    查看更多>>摘要：Objective:To investigate whether cardiac mast cells(MCs)participate in pressure overload-induced myocardial hypertrophy through the regulation of transient receptor potential vanilloid 4(TRPV4).Methods:Pressure overload-induced myocardial hypertrophy was induced via abdominal aortic constriction(AAC).Myocardial hypertrophy was evaluated by measuring the heart weight index(HW/BW),lung weight index(LW/BW),ratio of heart weight to tibia length(HW/TL),ratio of lung weight to tibia length(LW/TL),and cross-sectional area of myocardial cells.qRT-PCR was used to detect the mRNA expression of TRPV4.Western blotting was used to detect the protein expression of TRPV4,mast cell tryptase,myosin heavy chain beta(β-MHC),calcineurin A(CnA),and nuclear factor of activated T-cell c3(NFATc3).ELISA was used to measure the levels of brain natriuretic peptide(BNP)and histamine.Fluo4 AM was used to detect the calcium signal in H9c2 myocardial cells.Results:Compared with those of the sham rats,the myocardial mast cells,tryptase,HW/BW,LW/BW,HW/TL,and LW/TL,the cross-sectional area of the myocardial cells,and the expression of β-MHC,TRPV4,CnA,and NFATc3 in the myocardial tissue and the serum BNP of the AAC-treated rats increased significantly,whereas the MC stabilizer cromolyn sodium(CS)reversed these indicators.In H9c2 cardiomyocytes,treatment with histamine and the TRPV4 agonist GSK1016790A upregulated the expression of TRPV4,β-MHC,BNP,CnA and NFATc3 and increased calcium ion influx,whereas these effects were inhibited by the H2 receptor inhibitor famotidine and the TRPV4 inhibitor HC067047.Conclusion:Cardiac MCs participate in pressure overload-induced myocardial hypertrophy through the upregulation of TRPV4 via its mediator histamine,and the Ca2+/CnA/NFATc3 signaling pathway is involved in this process.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Qiliqiangxin Alleviates Imbalance of Inflammatory Cytokines in Patients with Dilated Cardiomyopathy:A Randomized Controlled Trial

    Yu-hua LIAOJing YUANXue-juan JINYing-zhen YANG...
    1081-1090页
    查看更多>>摘要：Objective:Qiliqiangxin(QLQX)capsule,a traditional Chinese medicine used for treating heart failure(HF),can modulate inflammatory cytokines in rats with myocardial infarction.However,its immune-regulating effect on dilated cardiomyopathy(DCM)remains unknown.The aim of this study was to investigate whether QLQX has a unique regulatory role in the imbalance of pro-and anti-inflammatory cytokines in patients with DCM.Methods:The QLQX-DCM is a randomized,double-blind trial conducted at 24 tertiary hospitals in China.A total of 345 patients with newly diagnosed virus-induced DCM were randomly assigned to receive QLQX capsules or placebo while receiving optimal medical therapy for HF.The primary endpoints were changes in plasma inflammatory cytokines and improvements in left ventricular ejection fraction(LVEF)and left ventricular end-diastolic diameter(LVEDd)over the 12-month treatment.Results:At the 12-month follow-up,the levels of IFN-γ,1L-17,TNF-α,and IL-4 decreased significantly,while the level of IL-10 increased in both groups compared with baselines(all P＜0.0001).Furthermore,these changes,coupled with improvements in LVEF,NT-proBNP and New York Heart Association(NYHA)functional classification,excluding the LVEDd in the QLQX group,were greater than those in the placebo group(all P＜0.001).Additionally,compared with placebo,QLQX treatment also reduced all-cause mortality and rehospitalization rates by 2.17％and 2.28％,respectively,but the difference was not statistically significant.Conclusion:QLQX has the potential to alleviate the imbalance of inflammatory cytokines in patients with DCM,potentially leading to further improvements in cardiac function when combined with anti-HF standard medications.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    A Novel Method for Mitochondrial Membrane Potential Detection in Heart Tissue Following Ischemia-reperfusion in Mice

    Chao YINChen-xing HUANGLe PANKe-jia JIN...
    1091-1096页
    查看更多>>摘要：Objective:Myocardial ischemia-reperfusion(I/R)injury is associated with a significant reduction in the mitochondrial membrane potential(MMP,△Ψm).Fluorescence-based assays are effective for labelling active mitochondria in living cells;their application in heart tissue,however,represents a challenge because of a low yield of viable cardiomyocytes after cardiac perfusion.This study aimed to examine a novel method for detecting the changes in the MMP of mouse heart tissue following I/R injury.Methods:The I/R model was established,which was characterized by distinct ischemic area and apoptosis in heart tissue.The MMP was detected via a confocal microscope after the ascending aorta was clamped and the mitochondrial probe solution(containing Mito-Tracker Deep Red FM)was perfused from the apex via a peristaltic pump.Results:This method enabled the distribution of the probe solution throughout the cardiac tissue via the coronary circulation.Fluorescence detection revealed that the MMP was profoundly reduced in both ischemic area and border area following I/R when compared with that in the sham group.There was no obvious difference in the MMP of the remote area between the I/R group and the sham group.Conclusion:This study presents a novel method for detecting the MMP in heart tissue,and this method will facilitate the evaluation of changes in the MMP in different regions following I/R.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Statins Combined with AAV8-TBG-LOX-1 Reduce the Vascular Lipid-driven Inflammatory Response and Inhibit Atherosclerosis

    Wen-ping ZHOUXin-rui FANSong-hai LIZhuang-lin ZENG...
    1097-1102页
    查看更多>>摘要：Objective:Imbalances in liver lipid metabolism and inflammatory reactions driven by oxidized lipid deposition in blood vessels constitute the core of atherosclerosis.Insufficient degradation of cholesterol in the liver promotes oxidative modification of lipid particles and their deposition on the blood vessel wall in the peripheral circulation.The blood vessel wall engulfs and processes oxidized low-density lipoprotein(Ox-LDL)as foreign matter through pattern recognition receptors,ultimately forming lipid-encapsulated plaques.Among them,endothelial cell oxidized low density lipoprotein receptor 1(LOX1)phagocytosis is an important link in initiating and promoting this mechanism,and hepatocytes,which are the core of lipid metabolism,are unable to process oxidized lipid particles because of the lack of receptors for the uptake of Ox-LDL.The objective of this study was to investigate whether continuous clearance of Ox-LDL through the liver metabolic pathway could provide better protection against statins therapy.Methods:This study used statins combined with an adeno-associated virus(AAV8-TBG-LOX-1)liver-specific transfection system developed by our research group,in which statins reduced the level of LDL and promoted the ectopic expression of LOX-1 in hepatocytes to clear the continuous production of Ox-LDL.An ApoE knockout mouse model was used to study the effects of virus transfection and liver uptake and degradation of Ox-LDL.Laser confocal detection,Oil red staining and immunofluorescence staining were used to observe the effects of combined therapy on anti-atherosclerotic lesions.Results:Laser confocal microscopy revealed that the recombinant viral vector AAV8-TBG-LOX-1 could specifically transfect hepatocytes and express LOX-1,which mediate hepatocyte phagocytosis and clearance of Ox-LDL.Oil red O staining of the aorta and valvular ring suggested that statins combined with AAV8-TBG-LOX-1 significantly inhibited atherosclerotic lesions.Tissue immunofluorescence staining suggested that statins could reduce the aggregation of macrophages in plaques and that combined therapy could further reduce the aggregation of macrophages in plaques.Conclusion:Statins combined with AAV8-TBG-LOX-1 can alleviate the inflammatory response driven by lipids in the vascular wall,reduce the deposition of macrophages in plaques and inhibit atherosclerosis.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Effect of Continuous Erector Spinae Plane Block on Postoperative Recovery in Patients Undergoing Minimally Invasive Cardiac Surgery:A Prospective,Randomized Controlled Clinical Trial

    Lin JINYing YUPeng MIAOYi-hao HUANG...
    1103-1112页
    查看更多>>摘要：Objective:To investigate whether continuous erector spinae plane block(ESPB)improves the quality of recovery(QoR)and decreases postoperative acute and chronic pain in patients undergoing minimally invasive cardiac surgery.Methods:This was a single-center,double-blind,prospective,randomized,placebo-controlled trial.A total of 120 patients were randomized to groups at a 1∶1 ratio.They received general anaesthesia and an ESP catheter(ropivacaine or normal saline)before surgery,and received patient-controlled intravenous analgesia with sufentanil and continuous ESPB with a pulse injection of 8 mL(ropivacaine or normal saline)per h after 20 mL of the experimental drug was administered at the end of surgery.The primary outcome was the 15-item quality of recovery scale(QoR-15)score at 24 h after surgery.The secondary outcomes included the severity of pain,sufentanil consumption,incidence of rescue analgesia,and proportion of patients with chronic pain.Results:The QoR-15 score was greater in the ESPB group than in the control group at 24 h after surgery[112(108-118)vs.109(101-114),P=0.023].ESPB was associated with a lower cough visual analogue scale(VAS)score(44 vs.47,P=0.001),resting VAS score(28 vs.35.5,P=0.003),sufentanil consumption(104.8 μg vs.145.5 μg,P=).000),and incidence of rescue analgesia(20.0％vs.43.3％,P=0.006).Conclusion:Continuous ESPB mildly improved the QoR-15 score in patients undergoing minimally invasive cardiac surgery and reduced postoperative pain scores,opioid consumption,and the incidence of rescue analgesia.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Artificial Intelligence in Medical Meta verse:Applications,Challenges,and Future Prospects

    Jia-ming YANGBao-jun CHENRui-yuan LIBi-qiang HUANG...
    1113-1122页
    查看更多>>摘要：The medical metaverse is a combination of medicine,computer science,information technology and other cutting-edge technologies.It redefines the method of information interaction about doctor-patient communication,medical education and research through the integration of medical data,knowledge and services in a virtual environment.Artificial intelligence(AI)is a discipline that uses computer technology to study and develop human intelligence.Al has infiltrated every aspect of medical metaverse and is deeply integrated with the technologies that build medical metaverse,such as large language models(LLMs),digital twins,blockchain and extended reality(including VR/AR/XR).AI has become an integral part of the medical metaverse building process.Moreover,AI also provides richer medical metaverse functions,including diagnosis,education,and consulting.This paper aims to introduce how AI supports the development of medical metaverse,including its specific application scenarios,shortcomings and future development.Our goal is to contribute to the advancement of more sophisticated and intelligent medical methods.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Brain-computer Interaction in the Smart Era

    Zi-neng YANPeng-ran LIUHong ZHOUJia-yao ZHANG...
    1123-1131页
    查看更多>>摘要：The brain-computer interface(BCI)system serves as a critical link between external output devices and the human brain.A monitored object's mental state,sensory cognition,and even higher cognition are reflected in its electroencephalography(EEG)signal.Nevertheless,unprocessed EEG signals are frequently contaminated with a variety of artifacts,rendering the analysis and elimination of impurities from the collected EEG data exceedingly challenging,not to mention the manual adjustment thereof.Over the last few decades,the rapid advancement of artificial intelligence(AI)technology has contributed to the development of BCI technology.Algorithms derived from AI and machine learning have significantly enhanced the ability to analyze and process EEG electrical signals,thereby expanding the range of potential interactions between the human brain and computers.As a result,the present BCI technology with the help of AI can assist physicians in gaining a more comprehensive understanding of their patients'physical and psychological status,thereby contributing to improvements in their health and quality of life.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Application and Prospects of Deep Learning Technology in Fracture Diagnosis

    Jia-yao ZHANGJia-ming YANGXin-meng WangHong-lin WANG...
    1132-1140页
    查看更多>>摘要：Artificial intelligence(AI)is an interdisciplinary field that combines computer technology,mathematics,and several other fields.Recently,with the rapid development of machine learning(ML)and deep learning(DL),significant progress has been made in the field of AI.As one of the fastest-growing branches,DL can effectively extract features from big data and optimize the performance of various tasks.Moreover,with advancements in digital imaging technology,DL has become a key tool for processing high-dimensional medical image data and conducting medical image analysis in clinical applications.With the development of this technology,the diagnosis of orthopedic diseases has undergone significant changes.In this review,we describe recent research progress on DL in fracture diagnosis and discuss the value of DL in this field,providing a reference for better integration and development of DL technology in orthopedics.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Wearable EEG Neurofeedback Based-on Machine Learning Algorithms for Children with Autism:A Randomized,Placebo-controlled Study

    Xian-na WANGTong ZHANGBi-cheng HANWei-wei LUO...
    1141-1147页
    查看更多>>摘要：Objective:Behavioral interventions have been shown to ameliorate the electroencephalogram(EEG)dynamics underlying the behavioral symptoms of autism spectrum disorder(ASD),while studies have also demonstrated that mirror neuron mu rhythm-based EEG neurofeedback training improves the behavioral functioning of individuals with ASD.This study aimed to test the effects of a wearable mu rhythm neurofeedback training system based on machine learning algorithms for children with autism.Methods:A randomized,placebo-controlled study was carried out on 60 participants aged 3 to 6 years who were diagnosed with autism,at two center-based intervention sites.The neurofeedback group received active mu rhythm neurofeedback training,while the control group received a sham neurofeedback training.Other behavioral intervention programs were similar between the two groups.Results:After 60 sessions of treatment,both groups showed significant improvements in several domains including language,social and problem behavior.The neurofeedback group showed significantly greater improvements in expressive language(P=0.013)and cognitive awareness(including joint attention,P=0.003)than did the placebo-controlled group.Conclusion:Artificial intelligence-powered wearable EEG neurofeedback,as a type of brain-computer interface application,is a promising assistive technology that can provide targeted intervention for the core brain mechanisms underlying ASD symptoms.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Performance Assessment of GPT 4.0 on the Japanese Medical Licensing Examination

    Hong-lin WANGHong ZHOUJia-yao ZHANGYi XIE...
    1148-1154页
    查看更多>>摘要：Objective:To evaluate the accuracy and parsing ability of GPT 4.0 for Japanese medical practitioner qualification examinations in a multidimensional way to investigate its response accuracy and comprehensiveness to medical knowledge.Methods:We evaluated the performance of the GPT 4.0 on Japanese Medical Licensing Examination(JMLE)questions(2021-2023).Questions are categorized by difficulty and type,with distinctions between general and clinical parts,as well as between single-choice(MCQ1)and multiple-choice(MCQ2)questions.Difficulty levels were determined on the basis of correct rates provided by the JMLE Preparatory School.The accuracy and quality of the GPT 4.0 responses were analyzed via an improved Global Qualily Scale(GQS)scores,considering both the chosen options and the accompanying analysis.Descriptive statistics and Pearson Chi-square tests were used to examine performance across exam years,question difficulty,type,and choice.GPT 4.0 ability was evaluated via the GQS,with comparisons made via the Mann-Whitney U or Kruskal-Wallis test.Results:The correct response rate and parsing ability of the GPT4.0 to the JMLE questions reached the qualification level(80.4％).In terms of the accuracy of the GPT4.0 response to the JMLE,we found significant differences in accuracy across both difficulty levels and option types.According to the GQS scores for the GPT 4.0 responses to all the JMLE questions,the performance of the questionnaire varied according to year and choice type.Conclusion:GTP4.0 performs well in providing basic support in medical education and medical research,but it also needs to input a large amount of medical-related data to train its model and improve the accuracy of its medical knowledge output.Further integration of ChatGPT with the medical field could open new opportunities for medicine.
      原文链接:
    • NETL
    • NSTL
    • 万方数据