查看更多>>摘要：Background:The exudative form of age-related macular degeneration(AMD)is characterized by abnormal blood vessel growth,which is stimulated by vascular endothelial growth factor(VEGF)released from retinal pigment epithe-lium(RPE).The angiogenic behaviors of vascular endothelial cells in vitro depend on forkhead box protein P1(Foxp1),a transcription repressor widely expressed in human and murine tissues during development.In this study,we aimed to determine whether loss of Foxp1 affects laser-induced choroidal neovascularization(CNV)in mouse.Methods:Eye-selective deletion of Foxp1 was obtained by crossing Foxp1flox/flox with Six3-Cre mice.Laser pho-tocoagulation was delivered to 6-to 8-week-old mice to induce CNV.The expression of Foxp1 and Cre was determined by immunofluorescence in cryostat sections of the eyes.Fundus fluorescein angiography(FFA),optical coherence tomography(OCT),and B4 isolectin staining were applied to analyze the leakage,bulge height,and area of CNV lesions,respectively.RPE-choroid tissues were isolated for the determination of VEGF and pigment epithelium derived factor(PEDF)by Western blotting.Results:Foxp1 was expressed in retinal ganglion cells,RPEs,and the choroidal endothelial cells.Laser photocoagula-tion increased the number of Foxp1-endothelial cells and induced CNV.Six3-Cre reduced Foxp1 expression in RPE but not the endothelium,leading to a lower level of VEGF in the RPE-choroid.Foxp1 knockout inhibited pathological angiogenesis and vascular leakage of the laser-induced CNV lesions.Conclusions:Foxp1 regulates the expression of VEGF in the RPE,and inhibition of Foxp1 could potentially be a novel strategy for the prevention and therapy of neovascularization related to AMD.
查看更多>>摘要：Background:To investigate the optical coherence tomography angiography(OCTA)characteristics of diabetic macular edema(DME)at different stages.Methods:This study was a cross-sectional study.Patients diagnosed with DME were recruited.DME was classified into early,advanced,and severe DME.The vessel density(VD)in the superficial vascular plexus(SVP),deep vascular plexus(DVP)and foveal avascular zone(FAZ)parameters,including FAZ area,FAZ perimeter,acircularity index and foveal VD in a 300-μm-wide region around the FAZ(FD-300),were calculated by the AngioVue software.A multivariate generalized estimating equation was used to evaluate the associations between visual acuity and OCTA metrics.Results:Ninety-two eyes from 74 patients with DME were included in this study.Compared to early(P=0.006)and advanced DME(P=0.003),the acircularity index was higher in severe DME.Both whole and parafoveal VD in the DVP decreased in eyes with severe DME compared to early DME(P=0.018,P=0.005,respectively)and advanced DME(P=0.035,P=0.012,respectively).In the multivariate generalized estimating equation,DME severity,FAZ area and foveal thickness were positively associated with worse visual acuity(P=0.001,P=0.007 and P=0.001,respectively).Conclusion:Compared to early and advanced DME,severe DME showed increased irregularity in the FAZ and more extensive vessel damage in the DVP.Greater severity level of DME,larger FAZ area,and increased foveal thickness could be risk factors for poor visual acuity.
查看更多>>摘要：Background:To evaluate whether the specific choroidal neovascularization(CNV)characteristics measured using optical coherence tomography angiography(OCTA)can predict the 6-month prognosis of neovascular age-related macular degeneration(nAMD)after anti-vascular endothelial growth factor(anti-VEGF)therapy.Methods:Patients with type 1,type 2,or mixed-type neovascularization(NV)were prospectively included.Partici-pants underwent an initial loading phase of three consecutive monthly intravitreal injections of Conbercept(0.5 mg)and were switched to a pro re nata(PRN)treatment strategy.OCTA images were evaluated for eyes that underwent follow-up assessments for more than 6 months.CNV lesions were manually segmented,and the CNV area,vessel area,greatest vascular caliber(GVC),and greatest linear dimension(GLD)were compared between responders and non-responders.Two masked graders independently measured the above-mentioned parameters using OCTA,and consistency was assessed using the intraclass correlation coefficient(ICC)values.Multiple logistic regression analysis was performed to evaluate the effect of a 3-month change in the CNV area,GLD,and GVC on the 6-month response to anti-VEGF agents.Results:Among the 60 eyes of 60 patients with nAMD,39 were responders and 21 were non-responders.The pro-portion of CNV types was significantly different between responders and non-responders(P=0.009).Patients with type 2 or mixed NV seemed more likely to respond to the treatment(28.2％vs.0.0％,and 30.8％vs.23.8％,respectively).The change in GVC showed a significant difference between responders(-4.98±17.17 pm)and non-responders(11.01±14.10 pm)after three monthly intravitreal anti-VEGF injections.Multiple logistic regression analysis showed that only the change in GVC remained significant after controlling for baseline GVC,injection number,and CNV type(adjusted OR=1.083;P=0.008).Conclusions:Type 2 and mixed-type NV were significantly associated with a better response to anti-VEGF therapy.Changes in GVC after 3 months of treatment were significantly associated with a response to anti-VEGF therapy at 6 months.
查看更多>>摘要：Background:To investigate the effects of lighting conditions and accommodation on the three-dimensional posi-tion of Visian implantable collamer lens(ICL V4c).Methods:This observational study recruited 62 eyes of 31 myopia patients underwent ICL V4c implantation.Anterior segment optical coherence tomography(AS-OCT)assessed the anterior chamber depth(ACD),ACD-ICL(distance from the corneal endothelium to anterior surface of the ICL V4c),vault(distance between the posterior ICL V4c surface and anterior crystalline lens surface),and crystalline lens tilt under various lighting conditions and accom-modation relative to the corneal topographic axis at one year after ICL V4c implantation.Baseline was defined as the scotopic condition,which was also the non-accommodative stimulus condition.The ICL V4c tilt was analyzed using MATLAB.The significance level was set at P＜0.05.Results:The ACD-ICL values were similar under various lighting conditions(P=0.978)but decreased during accom-modation(P＜0.001).The vault was significantly smaller under mesopic and photopic conditions than the baseline(P=0.044 and P＜0.001,respectively)but remained unchanged during accommodation(P=0.058).The inferotem-poral proportion of ICL V4c(88.7％,55 eyes)and crystalline lens(74.2％,46 eyes)tilts were not significantly different(P=0.063).Crystalline lens under various lighting conditions and accommodation exhibited similar tilts.The vertical tilt of ICL V4c was significantly larger under photopic conditions than the baseline(P=0.038).The horizontal and total tilts were significantly decreased during accommodation(P=0.043 and 0.013,respectively).Conclusions:The axial position of ICL V4c in the anterior chamber was stable under various lighting conditions.Lighting conditions and accommodation may influence vertical,horizontal and total tilts of ICL V4c.
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