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中草药（英文版）
中草药（英文版）

季刊

1674-6384

chm@tiprpress.com;bjchm@tiprpress.com

022-23006901

300193

天津市南开区鞍山西道308号

中草药（英文版）/Journal Chinese Herbal MedicinesCSTPCD
正式出版
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    Metabolomics combined with network pharmacology reveals anti-asthmatic effects of Nepeta bracteata on allergic asthma rats

    Kailibinuer AbulaitiMiheleayi AikepaMireguli AinaiduJiaxin Wang...
    599-611页
    查看更多>>摘要：Objective:To investigate the mechanisms that underlie the anti-asthmatic effects of Nepeta bracteata(DBJJ,Dabao Jingjie in Chinese)in rats by integrating metabolomics and network pharmacology.Methods:In this study,the rat model of asthma was induced by ovalbumin(OVA),and the rats were trea-ted with a decoction of N.bracteata.Pathological changes in lung tissue were observed,and the quantifi-cation of eosinophils(EOS)and white blood cells(WBC)in bronchoalveolar lavage fluid was performed.Furthermore,the serum levels of asthma-related factors induced by OVA were assessed.1H NMR spec-troscopy serum metabolomics method was utilized to identify differential metabolites and their associ-ated metabolic pathways.UPLC-QE-MS/MS combined with network pharmacology was employed to predict the core targets and pathways of DBJJ in its action against asthma.The anti-asthmatic properties of DBJJ were investigated using an integrated approach of metabolomics and network pharmacology.The findings were validated through molecular docking and Western blotting analysis of the key targets.Results:The administration of DBJJ effectively alleviated OVA-induced lung histopathological changes and decreased the number of EOS and WBC in BALF.Additionally,DBJJ inhibited the OVA-induced eleva-tion of TNF-α,IL-18,Ig-E,EOS,IL-1 β,MDA,VEGF-A,and TGF-β1.A total of 21 biomarkers and 10 pathways were found by metabolomics analysis.A total of 29 compounds were identified by UPLC-QE-MS/MS,in which 13 active components were screened by oral availability and Caco-2 cell permeability,the 120 tar-gets and 173 KEGG pathways were predicted.The integration of metabolomics and network pharmaco-logical analysis revealed that DBJJ's main constituents,including ferulic acid and ursolic acid,exerted their effects on four targets,namely DAO and NOS2,as well as their associated metabolites and pathways.The active constituents of DBJJ demonstrated a high binding affinity towards DAO and NOS2.Furthermore,DBJJ was observed to decrease the protein expression and phosphorylation levels of NOS2,MAPK,and STAT3.Conclusion:The administration of DBJJ demonstrates notable anti-asthma properties in rats with allergic asthma.This effect can be attributed to the modulation of various targets,including NOS2,MAPK,and STAT3,by primary constituents such as ferulic acid and ursolic acid.
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    Deciphering relationship between depression and microbial molecules based on multi-omics:A case study of Chaigui Granules

    Qi WangYingxia ZhaoXuemei QinJunsheng Tian...
    612-621页
    查看更多>>摘要：Objective:To decipher the antidepression effect of Chaigui Granules(CGKL)from the relationship between depression and microbial molecules based on multi-omics.Methods:Male SD rats were subjected to chronic unpredictable mild stress(CUMS)for seven weeks.The antidepressants CGKL extract and CGKL were administered for the following four weeks.The behavior test and the content of monoamine neurotransmitters were used to evaluate the efficacy of CGKL.The 16S rRNA sequencing,LC-MS technology and molecular biological techniques were used to explore the pharmacological mechanism of CGKL.Results:CGKL treatment obviously alleviated the depressive behavioral indicators and regulated the con-tent of monoamine neurotransmitters,and presented dose-dependent manner.CGKL could also improve the arginine metabolism disorder of gut microbiota in the jejunum.Meanwhile,the contents of arginine and its metabolites in the serum and hippocampus were regulated to normal levels.Further investigation indicated that the expression of related rate-limiting enzyme genes and proteins in the hippocampus was validated by qRT-PCR and Western blotting.The results showed that the gut microbiota,metabolites,and genes or proteins of rate-limiting enzymes involved in the arginine pathway were significantly regulated by CGKL.Conclusion:The present study demonstrates that CGKL might exert antidepressant effects through regu-lating arginine metabolism,and its mechanism may be related to modulating the gut microbiota and related metabolic enzyme.
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    Compound Danshen Dripping Pills combined with isosorbide mononitrate for angina pectoris:A systematic review and a Meta-analysis

    Ru WangJing HuYuanyuan LiHong Yin...
    622-637页
    查看更多>>摘要：Objective:To evaluate the efficacy of Compound Danshen Dripping Pills(CDDP)combined with isosor-bide mononitrate(ISMN)versus ISMN alone for treating angina pectoris in patients.Methods:The PubMed,Web of Science,Cochrane Library,Embase China National Knowledge Infrastructure,China Biomedical Literature Service System,Chinese Medical Journal Database,and Wan Fang MED databases were searched from inception to November 2022.Randomized controlled trials(RCTs)and cohort studies were included.The primary outcomes were angina symptom and electrocar-diography(ECG)efficacy,angina symptom efficacy,and ECG efficacy.The protocol was registered with PROSPERO No.CRD42022314774.Results:Our study included 7 245 patients with angina(59 RCTs,11 cohort studies).When ISMN was combined with CDDP,the efficacy of angina symptom and ECG[odds ratio(OR)=4.824,95％confidence interval(CI)=3.636-6.401,P=0.000],the efficacy of angina symptom(OR=4.347,95％CI=3.635-5.198,P=0.000),the efficacy of ECG(OR=3.364,95％CI=2.767-4.089,P=0.000)were better than that of patients treated with ISMN alone.CDDP combined with ISMN was superior to ISMN alone in reducing triglyceride(TG)[mean difference(MD)=-35.176,95％CI=-37.439 to-32.912,P=0.000],total cholesterol(TC)(MD=-24.296,95％CI=-26.429 to-22.163,P-0.000),the duration of angina attack(MD=-1.991,95％CI=-2.349 to-1.633,P=0.000),and the frequency of angina attack[standardized MD(SMD)=-2.840,95％CI=-3.416 to-2.265,P=0.000].There was no increase in adverse events between CDDP combined with ISMN and ISMN alone(OR=0.513,95％CI=0.421-0.626,P=0.000).Conclusion:CDDP combined with ISMN improved treatment efficacy and was well tolerated.Therefore,this combination could be used as an alternative treatment.However,clinical and patient conditions should be considered.
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    Network pharmacology,molecular docking,and untargeted metabolomics reveal molecular mechanisms of multi-targets effects of Qingfei Tongluo Plaster improving respiratory syncytial virus pneumonia

    Mengfei YangXiuying ZhangQing LiuYongxue Wang...
    638-655页
    查看更多>>摘要：Objective:Qingfei Tongluo Plaster(QFP),an improved Chinese medicine hospital preparation,is an attractive treatment option due to its well clinical efficacy,convenience,economy,and patient compli-ance in the treatment of respiratory syncytial virus(RSV)pneumonia.The aim of this study was to inves-tigate the efficacy mechanism of QFP on RSV rats from the perspective of alleviating lung inflammation and further explore the changes of serum metabolites and metabolic pathways in RSV rats under the influence of QFP.Methods:This study used network pharmacological methods and molecular docking combined with molecular biology and metabolomics from multi-dimensional perspectives to screen and verify the ther-apeutic targets.Open online databases were used to speculate the gene targets of efficient ingredients and diseases.Then,we used the String database to examine the fundamental interaction of common tar-gets of drugs and diseases.An online enrichment analysis was performed to predict the functional path-ways.Molecular docking was applied to discover the binding modes between essential ingredients and crucial gene targets.Finally,we demonstrated the anti-inflammatory ability of QFP in the RSV-evoked pneumonia rat model and explained the mechanism in combination with the metabolomics results.Results:There were 19 critical targets defined as the core targets:tumor necrosis factor(TNF),inducible nitric oxide synthase 2(NOS2),mitogen-activated protein kinase 14(MAPK14),g1/S-specific cyclin-D1(CCND1),signal transducer and activator of transcription 1-alpha/beta(STAT1),proto-oncogene tyrosine-protein kinase Src(SRC),cellular tumor antigen p53(TP53),interleukin-6(1L6),hypoxia-inducible factor 1-alpha(HIF1A),RAC-alpha serine/threonine-protein kinase(AKT1),signal transducer and activator of transcription 3(STAT3),heat shock protein HSP 90-alpha(HSP90AA1),tyrosine-protein kinase JAK2(JAK2),cyclin-dependent kinase inhibitor 1(CDKN1A),mitogen-activated protein kinase 3(MAPK3),epidermal growth factor receptor(EGFR),myc proto-oncogene protein(MYC),protein c-Fos(FOS)and transcription factor p65(RELA).QFP treated RSV pneumonia mainly through the phosphatidyli-nositol 3-kinase(PI3K)/RAC AKT pathway,HIF-1 pathway,IL-17 pathway,TNF pathway,and MAPK path-way.Animal experiments proved that QFP could effectively ameliorate RSV-induced pulmonary inflammation.A total of 28 metabolites underwent significant changes in the QFP treatment,and there are four metabolic pathways consistent with the KEGG pathway analyzed by network pharmacology,suggesting that they may be critical processes related to treatment.Conclusion:These results provide essential perspicacity into the mechanisms of action of QFP as a promising anti-RSV drug.
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    Mechanisms of Shufeng Jiedu Capsule in treating bacterial pneumonia based on network pharmacology and experimental verification

    Yingli XuLei BaoRonghua ZhaoZihan Geng...
    656-666页
    查看更多>>摘要：Objective:The aim of this study was to investigate the underlying mechanism of Shufeng Jiedu Capsule(SFJD)for treating bacterial pneumonia(BP)in vivo based on network pharmacology and experimental verification study.Methods:Network pharmacology was used to screen the active compounds and target genes of SFJD.Then,the multi drug resistance-Pseudomonas aeruginosa(MDR-PA)mice lethal model and MDR-PA pneu-monia model were established to evaluate the therapeutic effects and underlying mechanisms of SFJD.Western blot and ELISA were used to determinate the protein expression level of the IL-17 signaling path-way and JAK/STAT signaling pathway.Results:After screening,172 potential components of SFJD were generated,based on which we con-structed an SFJD-component-target-BP interaction network.The Gene ontology(GO)and Kyoto encyclo-pedia of genes and genomes(KEGG)enrichment revealed that SFJD could regulate the IL-17 signaling pathway and Janus kinase/signal transducer and activator of transcription(JAK/STAT)signaling pathway.Molecular docking showed that the potential target proteins had good combinations with the main active components.SFJD significantly reduced the mortality and prolonged survival days in lethal models.The lung index and pathological changes in the lung were also significantly decreased.SFJD could signifi-cantly decrease the expression of interleukin-17A(IL-17A),TNF receptor associated factor 6(TRAF6),phospho-inhibitor of nuclear factor-kappa B(p-IKB)/inhibitor of NF-κB(IκB),phospho-NF-κB p65(p-NF-κB p65),phospho-protein kinase B(p-AKT)/AKT,phospho-signal transducer and activator of tran-scription 3(p-STAT3)/STAT3,phospho-signal transducer and activator of transcription 1(p-STAT1)/STAT1,and the protein level of interleukin-6(IL-6),tumor necrosis factor α(TNF-α),and IL-1β.Conclusion:Combined with network pharmacology and in vivo study,it was found that SFJD exerted its therapeutic effects on BP by inhibiting the IL-17 pathway and JAK/STAT signaling pathway.This study provides new evidence for SFJD in treatment of BP.
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    Inula britannica ameliorates alcohol-induced liver injury by modulating SIRT1-AMPK/Nrf2/NF-κB signaling pathway

    Zhennan MengMengyuan LiXiaoli WangKuo Zhang...
    667-678页
    查看更多>>摘要：Objective:Inula britannica is a traditional Chinese medicinal and functional food with various effects such as anti-liver injury,hypoglycemia,antioxidants,and anti-tumor.The aim of this study was to investigate the protective effects and mechanisms of the ethanolic extract of I.britannica(EEIB)on alcohol-induced liver injury in mice.Methods:Fifty-six female C57BL/6 mice were randomly divided into seven groups:control group(Con),ethanol feeding model group(EtOH),Silibinin positive treatment group(EtOH+Silibinin 100 mg/kg),EEIB treatment group(EtOH+EEIB 100,200,and 400 mg/kg),and EEIB control group(EEIB 400 mg/kg).The National Institute on Alcohol Abuse and Alcoholism(NIAAA)ethanol-feeding model was used to study the effects of EEIB on alcohol-induced lipid metabolism,inflammation,oxidative stress,and fibril formation in mice by histopathological evaluation,immunofluorescence staining,Western blotting anal-ysis and molecular docking.Results:EEIB reduced liver indices to different degrees to normal levels and improved liver morphology in mice.EEIB inhibited alcohol-induced liver injury by activating the sirtuin 1(S1RT1)-adenosine monophosphate-activated protein kinase(AMPK)signaling pathway in the liver of alcohol-fed mice,in which sesquiterpenes may be the potential active ingredients,and also down-regulated the expression of alpha-smooth muscle actin(α-SMA),collagen alpha(Collagen I),tumor necrosis factor-alpha(TNF-α)and attenuated alcohol-induced liver injury.In addition,EEIB also activated the nuclear factor ery-throid 2-related factor 2(Nrf2)signaling pathway,which alleviated alcohol-induced liver injury at the level of oxidative stress.Notably,the EEIB control group demonstrated that EEIB had no toxic effects in mice.EEIB reduced alcoholic liver injury in a dose-dependent manner.Its therapeutic efficacy was comparable to,if not better than,that of Silibinin when administered at a dose of 400 mg/kg.Conclusion:EEIB showed significant therapeutic effects on alcohol-induced liver injury in mice,and its mechanism of action was related to the SIRT1-AMPK,nuclear factor-kappa B(NF-κB),and Nrf2 signaling pathways,in which sesquiterpenes may be the potential active ingredients.
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    DNA metabarcoding uncovers fungal communities in Zingiberis Rhizoma

    Chune FanYanan XuYufeng LiMeihua Yang...
    679-685页
    查看更多>>摘要：Objective:Zingiberis Rhizoma(ZR,Ganjiang in Chinese),also known as dried ginger,is a popular spice and medicinal herb that has been used for several thousand years.However,ZR is easily contaminated by fungi and mycotoxin under suitable conditions,and might be hazardous to the health and safety of con-sumers,thus concerns about the herb's safety have been raised.The aim of this study was to investigate the fungal community and the effects of collection areas and processing methods on the fungal commu-nity in ZR.Methods:A total of 18 ZR samples were collected from four provinces of China,and the samples were divided into four groups based on collecting sites.Meanwhile,the samples collected in Sichuan Province,China were divided into three groups based on the processing methods.We employed the Illumina MiSeq PE300 platform and targeted the internal transcribed spacer 2(ITS2)sequences to inves-tigate fungal contamination in ZR samples,and the difference in fungal community among the groups of different collection sites and processing methods.Results:All 18 samples were contaminated with fungi.Ascomycota was the dominant phyla,accounting for 34.46％-100％of the fungal reads.At the genus level,Candida,Diutina,and Aspergillus were the most dominant genera,with relative abundances of 0-98.37％,0-99.82％,and 0-79.08％,respectively.Meanwhile,four potential toxigenic fungi and seven human pathogens were found.Furthermore,differ-ences in the community composition of ZR samples from four collecting sites and three processing meth-ods were observed.Conclusion:DNA metabarcoding provides a novel insight into fungal community diversity in ZR samples,providing references to ensure the sustainable utilization and quality research of ZR.
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