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中国农学前沿
高等教育出版社,Springer
中国农学前沿

高等教育出版社,Springer

季刊

1673-7334

100029

北京市朝阳区惠新东街4号富盛大厦15层

中国农学前沿/Journal Frontiers of Agriculture in China
查看更多>>涉及领域主要包括作物学、园艺学、植物保护、农业资源利用、畜牧、水产、兽医学、食品科学与加工等。
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收录年代

    Construction of the expression vector and location analysis of thermotolerant endoglucanase in E.Coli

    Runfang GUOKexue GAOHongwei YUYingmin JIA...
    72-76页
    查看更多>>摘要:To obtain the secreting expression vector, the signal peptide sequence and mature peptide sequence of endoglucanase from Streptomyces xylophagus KX6 were cloned into the pET28a plasmid. The recombinant vector pET28a/KX6 was transformed Escherichia coli Rosetta (DE3), and the transformant was induced by IPTG. The expression products were primarily distributed in the medium fluid of host cell in a soluble form and the activity was higher than that of other fractions. Both location analysis of targeting protein and activity analysis showed that the signal peptide of endoglucanase from S. xylophagus KX6 had played a very important role in the secret expression and activity of foreign proteins in the E. coli host cell.

    Do the tropical freshwater fishes feed on aquatic fungi?

    Kandikere R. SRIDHARNaga M. SUDHEEP
    77-86页
    查看更多>>摘要:Consumption and processing of allochthonous plant litter by fishes is more common in tropical than temperate streams and rivers. Therefore, aquatic hyphomycetes in water (filtration), leaf litter (bubble chamber incubation), and fecal pellets (direct observation and inoculation to sterile leaf litter) of three dominant fishes belonging to the family Cyprinidae (Aplocheilus lineatus, Puntius filamentosus, and Rasbora daniconius) in two locations of the River Kali of the Western Ghats, India, were evaluated during postmonsoon season. Spores of 14 and 9 species of aquatic hyphomycetes were recovered on filtering water samples of Kaiga stream and Kadra dam with equal number of spores (32 spores. 100 mL<'-1>) and high Shannon diversity in Kaiga stream. In a bubble chamber incubation of leaf litter, 16 and 9 species were recovered from Kaiga stream and Kadra dam with high spore output (1122 versus 324 spores per mg dry mass) and high Shannon diversity in Kaiga stream. Both direct and indirect methods of examination of fecal pellets of fishes revealed more species in Kaiga stream than Kadra dam (4-7 versus 1-4 species). The spore release in leaf litter incubated with fecal pellets ranged from 2.3 to 98 spores, mg<'-1>. d<'-1> with the highest Aplocheilus lineatus in Kaiga stream; while in Kadra dam, it was from 0.02 to 22.9 spores, mg<'-1>. d<'-1> with the highest in Puntius filamentosus. The Shannon diversity of aquatic hyphomycetes was high in fecal pellets of Aplocheilus lineatus of Kaiga stream and Rasbora daniconius of Kadra dam. The top-ranked five species of aquatic hyphomycetes differed in water, leaf litter, and fish fecal pellets; however, Triscelophorus konajensis was common for all. All five top-ranked species of aquatic hyphomycetes in feces produced multicelled spores; thus, they were likely to have a better chance of viability through gut passage than single-celled spores. Preferential feeding, fungi in gut and feces, and survival and dissemination of spores by invertebrates and fishes with reference to aquatic hyphomycetes were discussed.

    Genetic diversity and differentiation of four goat lineages based on analysis of complete mtDNA d-loop

    Jingfen KANGXianglong LIRongyan ZHOULanhui LI...
    87-93页
    查看更多>>摘要:The complete sequences of mtDNA D-loops from 362 individuals were analyzed in order to investigate the genetic diversity and differentiation of their lineages. The results indicated that all of the analyzed sequences were differentiated into four clear lineages (A, B, C, and D). Lineages C and D might originate from Lineages B and A, respectively. The genetic diversity of complete mtDNA D-loop of four lineages was very abundant. The 76 bp insertion and the 17 bp deletion were detected in the longest and the shortest sequences, respectively. The 76 bp insertion was a repeat like motif found in many other animals. Lineages C and D were differentiated into two subclades (C1 and C2) and (DI and D2), respectively. Lineage C might originate from Asia, and Lineage D might originate from Fertile Crescent.

    Effects of early feed restriction programs on production performance and hormone level in plasma of broiler chickens

    Lanhui LIGuoxian ZHAOZhiyou RENLei DUAN...
    94-101页
    查看更多>>摘要:Age, duration and intensity of feed restriction of broiler chickens were studied by L<,9>(3<'4>) orthogonal experiment. Broiler production performance and meat color were measured to evaluate the effects of feed restriction and explore the superior FR program. A total of 250 1-day-old Ross 308 chicks were selected and randomly allocated to ten groups, including one feed ad libitum (AL) group and nine feed restriction (FR) groups; each group had 5 pens with 5 chickens each. Broilers (T<,5>D<,7>I<,90>), feed-restricted to 90% from day 5 to day 11, had the highest body weight (BW), carcass weight, eviscerated weight, leg muscle weight and breast muscle weight, as well as muscle ratio. While broilers (T<,5>D<,14>I<,70>), feed-restricted to 70% from day 5 to day 18, had the lowest. Triiodothyronine (T<,3>) and growth hormone (GH) decreased while thyroxine (T<,4>) and the ratio of T<,4>/T<,3> increased in FR broilers. Feed conversion ratio, abdominal fat ratio, whole eviscerated weight ratio and lightness (L<'*>) and yellow/blue (b<'*>) value of leg muscle color increased in FR broilers (P>0.05). T<,5>D<,14>I<'70> had the lowest red/green (a<'*>) color value of leg muscle and the highest L<'*> and b<'*> color values of breast muscle (P>0.05). FR groups except T<,10>D<,14>I<,-90> had lower a<'*> color value of breast muscle than AL. FR program of T<,5>D<,7>I<,90> resulted in superior production performance and higher capability of meat production. Broilers feedrestricted T<,5>D<,14>I<,70> were stunted with the lowest muscle growth capability and deteriorated in the breast muscle color. FR improved leg muscle color and increased broiler byproduct production. Lower T<,3> and GH concentration and higher T<,4>/T<,3> ratio in plasma were related with the increased feed conversion rate and fat deposition in FR broilers; however, they had no relation with meat production and growth rate.

    Effects of traditional Chinese medicine "Yimu Shenghuatang" on cytochrome P450 in cow inflammatory endometrial cells

    Jinliang DUJianhua QINJingsheng CHULina XU...
    102-105页
    查看更多>>摘要:To investigate the metabolism mechanism of traditional Chinese medicine "Yimu Shenghuatang" on dairy cow endometrial cells, the primary cultured endometrial cells in dairy cows were isolated by a collagenase digestion method, and an inflammatory model of the endometrial cells by lipopolysaccharide (LPS) was made. The cells were treated with gradient concentrations of LPS, the cultured supernatants were collected in different periods, and the contents ofTNF-α, IL-1β were determined by Enzyme-linked immunosorbent assay (ELISA). The results showed that 100ng.mL<'-1> LPS was the optimal concentration to induce the inflammation of cultured endometrial cells. The inflammatory endometrial cells were treated with traditional Chinese medicine "Yimu Shenghuatang" and its main components during the period of 24-72 h, and the expression of cytochrome P450 in cow endometrial cells was determined by Western blot. Our result lays a foundation for treating cow endometritis by using traditional Chinese medicines.

    Structure, diversity, and regeneration potential of Monotheca buxifolia (Falc.) A.DC.dominated forests of Lower Dir District, Pakistan

    Nasrullah KHANMoinuddin AHMEDSyed Shahid SHAUKATMuhammad WAHAB...
    106-121页
    查看更多>>摘要:This study reports on the multivariate analysis of the vegetation of Hindukush Range in Pakistan, concentrating on the structure and regeneration potential of Monotheca buxifolia and associated tree species. Twenty stands at different locations in the Dir District of the Hindukush Range in Pakistan were chosen for the study. A point centered quarter method for trees and 5 m × 5 m size quadrats were used for the sampling of understorey vegetation, including shrubs, seedlings, and saplings, respectively. The underlying group structure in vegetation was exposed by an agglomerative clustering technique, while major Wends were disclosed by DCA ordination. Size class structure and regeneration potential of M. buxifolia and associated Wee species were also examined, which reflects the future Wend of species and, consequently, the forests where they dominate. The relationships between environmental factors and vegetation were investigated.The arboreal vegetation was mostly dominated by broad leaved species including Monotheca buxifolia, Olea ferruginea, Acacia modesta, Punica granatum, Quercus baloot, and Ficus palmata. Among the understorey vegetation, the abundant species were Dodonea viscosa, Justicia adhatoda, Otostegia limbata, Indigofera gerardiana, Plantago lanceolata, Rumex dentatus, Marrubium vulgaris, Fragaria nubicola, Geranium rotundifolium, Daphne oleoides, Solanum nigram, Ajuga bracteosa, Oxalis corniculata seedlings of Monotheca buxifolia, Quercus baloot, and Punica granatum. At the seedling and sapling stage, the maximum number was observed for Monotheca buxifolia (27±5.75 and 38±7.1), followed by Quercus baloot (18±2.2 and 12±1.0) and Olea ferruginea. As far as regeneration status is concerned, 34% species showed good regeneration, 50% species were facing the problem of poor regeneration while, and only 16% species were not regenerating. Five groups of tree vegetation that emerged from Ward's cluster analysis could readily be superimposed on DCA ordination. These groups were associated with particular elevation and, to a lesser extent, with edaphic variables, such as pH and nutrients. Some of the topographic and edaphic variables, such as soil nutrient, showed significant or weak linear relationships with one or more ordination axes. The size class structure of M. buxifolia and associated tree species for individual stands exhibited a few gaps. Relationships between density and basal area were significant, but the density and basal area with altitudinal and slope gradient showed an insignificant relation. Some recommendations are outlined for future research and sustainable management of these forests species.

    Influence of alginate oligosaccharides on growth, yield and alkaloid production of opium poppy (Papaver somniferum L.)

    Zeba H. KHANM. Masroor A. KHANTariq AFTABM. IDREES...
    122-127页
    查看更多>>摘要:Opium poppy (Papaver somniferum L.), an important medicinal plant, produces several opiate alkaloidsincluding morphine, thebaine, codeine, papaverine and noscapine. Polysaccharides, such as sodium alginate, have been used in depolymerized form as wonderful promoters of plant growth. The present study has revealed that application of alginate oligosaccharides (AO), obtained from sodium aiginate irradiated by Co-60 gamma rays, significantly enhances certain physiological/biochemical parameters as well as the overall growth of opium poppy. The highest dose applied was the most effective in increasing the morphine and codeine contents as well as the overall yield of crude opium per plant.