期刊,中国科学:生命科学（英文版） 2024年卷3期_国家学术搜索

期刊信息/Journal information

中国科学:生命科学（英文版）

主　　编：周光召

出版周期：月刊

国际刊号：1674-7305

电子邮箱：sales@scichina.org

电　　话：010-64019820

邮政编码：100717

地　　址：北京东黄城根北街16号

中国科学:生命科学（英文版）/Journal Science China(Life Sciences)CSCDCSTPCDSCI

查看更多>>《中国科学》是中国科学院主办、中国科学杂志社出版的自然科学专业性学术刊物。《中国科学》任务是反映中国自然科学各学科中的最新科研成果，以促进国内外的学术交流。《中国科学》以论文形式报道中国基础研究和应用研究方面具有创造性的、高水平的和有重要意义的科研成果。在国际学术界，《中国科学》作为代表中国最高水平的学术刊物也受到高度重视。国际上最具有权威的检索刊物SCI，多年来一直收录《中国科学》的论文。1999年《中国科学》夺得国家期刊奖的第一名。

正式出版

收录年代

Revealing the role of CCoAOMT1:fine-tuning bHLH transcription factors for optimal anther development

Zesen LaiJianzheng WangYing FuMenghan Wang...

565-578页

查看更多>>摘要：The tapetum,a crucial innermost layer encompassing male reproductive cells within the anther wall,plays a pivotal role in normal pollen development.The transcription factors(TFs)bHLH010/089/091 redundantly facilitate the rapid nuclear accumulation of DYSFUNCTIONAL TAPETUM 1,a gatekeeper TF in the tapetum.Nevertheless,the regulatory mechanisms governing the activity of bHLH010/089/091 remain unknown.In this study,we reveal that caffeoyl coenzyme A O-methyltransferase 1(CCoAOMT1)is a negative regulator affecting the nuclear localization and function of bHLHO10 and bHLH089,probably through their K259 site.Our findings underscore that CCoAOMT1 promotes the nuclear export and degradation of bHLHO10 and bHLH089.Intriguingly,elevated CCoAOMT1 expression resulted in defective pollen development,mirroring the phenotype observed in bhlh010 bhlh089 mutants.Moreover,our investigation revealed that the K259A mu-tation in the bHLH089 protein disrupted its translocation from the nucleus to the cytosol and impeded its degradation induced by CCoAOMT1.Importantly,transgenic plants with the probHLH089::bHLH089K259A construct failed to rescue proper pollen development or gene expression in bhlh010 bhlh089 mutants.Collectively,these findings emphasize the need to maintain balanced TF homeostasis for male fertility.They firmly establish CCoAOMT1 as a pivotal regulator that is instrumental in achieving equilibrium between the induction of the tapetum transcriptional network and ensuring appropriate anther development.

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万方数据

M6PR interacts with the HA2 subunit of influenza A virus to facilitate the fusion of viral and endosomal membranes

Yuzhen HuLi JiangGuangwen WangYangming Song...

579-595页

查看更多>>摘要：Influenza A virus(LAV)commandeers numerous host cellular factors for successful replication.However,very few host factors have been revealed to be involved in the fusion of viral envelope and late endosomal membranes.In this study,we identified cation-dependent mannose-6-phosphate receptor(M6PR)as a crucial host factor for the replication of IAV.We found that siRNA knockdown of M6PR expression significantly reduced the growth titers of different subtypes of IAV,and that the inhibitory effect of M6PR siRNA treatment on IAV growth was overcome by the complement of exogenously expressed M6PR.When A549 cells were treated with siRNA targeting M6PR,the nuclear accumulation of viral nucleoprotein(NP)was dramatically inhibited at early timepoints post-infection,indicating that M6PR engages in the early stage of the IAV replication cycle.By investigating the role of M6PR in the individual entry and post-entry steps of IAV replication,we found that the downregulation of M6PR expression had no effect on attachment,internalization,early endosome trafficking,or late endosome acidification,However,we found that M6PR expression was critical for the fusion of viral envelope and late endosomal membranes.Of note,M6PR interacted with the hemagglutinin(HA)protein of IAV,and further studies showed that the lumenal domain of M6PR and the ectodomain of HA2 mediated the interaction and directly promoted the fusion of the viral and late endosomal membranes,thereby facilitating IAV replication.Together,our findings highlight the importance of the M6PR-HA interaction in the fusion of viral and late endosomal membranes during IAV replication.

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万方数据
维普

Green manuring relocates microbiomes in driving the soil functionality of nitrogen cycling to obtain preferable grain yields in thirty years

Guopeng ZhouKunkun FanSongjuan GaoDanna Chang...

596-610页

查看更多>>摘要：Fertilizers are widely used to produce more food,inevitably altering the diversity and composition of soil organisms.The role of soil biodiversity in controlling multiple ecosystem services remains unclear,especially after decades of fertilization.Here,we assess the con-tribution of the soil functionalities of carbon(C),nitrogen(N),and phosphorus(P)cycling to crop production and explore how soil organisms control these functionalities in a 33-year field fertilization experiment.The long-term application of green manure or cow manure produced wheat yields equivalent to those obtained with chemical N,with the former providing higher soil functions and allowing the functionality of N cycling(especially soil N mineralization and biological N fixation)to control wheat production.The keystone phy-lotypes within the global network rather than the overall microbial community dominated the soil multifunctionality and functionality of C,N,and P cycling across the soil profile(0-100 cm).We further confirmed that these keystone phylotypes consisted of many metabolic pathways of nutrient cycling and essential microbes involved in organic C mineralization,N2O release,and biological N fixation.The chemical N,green manure,and cow manure resulted in the highest abundances of amoB,nifH,and GH48 genes and Nitrosomonadaceae,Azospirillaceae,and Sphingomonadaceae within the keystone phylotypes,and these microbes were significantly and positively correlated with N2O release,N fixation,and organic C mineralization,respectively.Moreover,our results demonstrated that organic fertilization increased the effects of the network size and keystone phylotypes on the subsoil functions by facilitating the migration of soil micro-organisms across the soil profiles and green manure with the highest migration rates.This study highlights the importance of the func-tionality of N cycling in controlling crop production and keystone phylotypes in regulating soil functions,and provides selectable fertilization strategies for maintaining crop production and soil functions across soil profiles in agricultural ecosystems.

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