期刊,中华医学杂志（英文版） 2024年卷11期_国家学术搜索

期刊信息/Journal information

中华医学杂志（英文版）

中华医学会

主办单位：中华医学会

主　　编：照日格图

出版周期：半月刊

国际刊号：0366-6999

电子邮箱：renlihua@cma.org.cn

电　　话：010-85158321

邮政编码：100710

地　　址：北京市东城区东四西大街42号

中华医学杂志（英文版）/Journal Chinese Medical JournalCSCDCSTPCD北大核心SCI

查看更多>>1887年创刊，中华医学会主办。中华医学杂志英文版(Chinese Medical Journal)是中华医学会会刊，是中国惟一被SCI核心版收录、具有百年以上历史的医学期刊。重点报道我国医学各学科最新进展和高水平科研成果，目前已被《科学引文索引（SCI）》、《医学索引（IM）》、Medline等国际著名检索系统收录。2009年SCI影响因子0.952，SCI被引频次3407。2010年被国际医学期刊编辑委员会（ICMJE）吸收为新成员。多次获得国家期刊奖、科协专项基金、自然基金等奖项和资助。实行全文上网（），网上投稿审稿()。

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Changes in muscle strength and risk of cardiovascular disease among middle-aged and older adults in China:Evidence from a prospective cohort study

Ze YangJiemin WeiHongbo LiuHonglu Zhang...

1343-1350页

查看更多>>摘要：Background:Evidence indicates that low muscle strength is associated with an increased cardiovascular diseases(CVDs)risk.However,the association between muscle strength changes based on repeated measurements and CVD incidence remains unclear.Methods:The study used data from the China Health and Retirement Longitudinal Study in 2011(Wave 1),2013(Wave 2),2015(Wave 3),and 2018(Wave 4).Low muscle strength was defined as handgrip strength＜28 kg for men or＜18 kg for women,or chair-rising time ≥12 s.Based on changes in muscle strength from Waves 1 to 2,participants were categorized into four groups of Normal-Normal,Low-Normal,Normal-Low,and Low-Low.CVD events,including heart disease and stroke,were recorded using a self-reported questionnaire during Waves 3 and 4 visits.Cox proportional hazards models were used to investigate the association between muscle strength changes and CVD incidence after multivariable adjustments.Hazard ratios(HRs)and 95％confidence intervals(95％CIs)were estimated with the Normal-Normal group as the reference.Results:A total of 1164 CVD cases were identified among 6608 participants.Compared to participants with sustained normal muscle strength,the CVD risks increased progressively across groups of the Low-Normal(HR=1.20,95％CI:1.01-1.43),the Normal-Low(HR=1.35,95％CI:1.14-1.60),and the Low-Low(HR=1.76,95％CI:1.49-2.07).Similar patterns were observed for the significant associations between muscle strength status and the incidence risks of heart disease and stroke.Subgroup analyses showed that the significant associations between CVD and muscle strength changes were consistent across age,sex,and body mass index(BMI)categories.Conclusions:The study found that muscle strength changes were associated with CVD risk.This suggests that continuous tracking of muscle status may be helpful in screening cardiovascular risk.

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国家科技期刊平台
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Direct reprogramming of fibroblasts into functional hepatocytes via CRISPRa activation of endogenous Gata4 and Foxa3

Jiacheng LiRuopu LiXue BaiWenlong Zhang...

1351-1359页

查看更多>>摘要：Background:The ability to generate functional hepatocytes without relying on donor liver organs holds significant therapeutic promise in the fields of regenerative medicine and potential liver disease treatments.Clustered regularly interspaced short palin-dromic repeats(CRISPR)activator(CRISPRa)is a powerful tool that can conveniently and efficiently activate the expression of multiple endogenous genes simultaneously,providing a new strategy for cell fate determination.The main purpose of this study is to explore the feasibility of applying CRISPRa for hepatocyte reprogramming and its application in the treatment of mouse liver fibrosis.Method:The differentiation of mouse embryonic fibroblasts(MEFs)into functional induced hepatocyte-like cells(iHeps)was achieved by utilizing the CRISPRa synergistic activation mediator(SAM)system,which drove the combined expression of three endogenous transcription factors—Gata4,Foxa3,and Hnf1a—or alternatively,the expression of two transcription factors,Gata4 and Foxa3.In vivo,we injected adeno-associated virus serotype 6(AAV6)carrying the CRISPRa SAM system into liver fibrotic Col1a1-CreER;Cas9fl/fl mice,effectively activating the expression of endogenous Gata4 and Foxa3 in fibroblasts.The endogenous transcriptional activation of genes was confirmed using real-time quantitative polymerase chain reaction(RT-qPCR)and RNA-seq,and the morphology and characteristics of the induced hepatocytes were observed through microscopy.The level of hepatocyte reprogramming in vivo is detected by immunofluorescence staining,while the improvement of liver fibrosis is evaluated through Sirius red staining,alpha-smooth muscle actin(α-SMA)immunofluorescence staining,and blood alanine aminotransferase(ALT)examination.Results:Activation of only two factors,Gata4 and Foxa3,via CRISPRa was sufficient to successfully induce the transformation of MEFs into iHeps.These iHeps could be expanded in vitro and displayed functional characteristics similar to those of mature hepatocytes,such as drug metabolism and glycogen storage.Additionally,AAV6-based delivery of the CRISPRa SAM system effectively induced the hepatic reprogramming from fibroblasts in mice with live fibrosis.After 8 weeks of induction,the repro-grammed hepatocytes comprised 0.87％of the total hepatocyte population in the mice,significantly reducing liver fibrosis.Conclusion:CRISPRa-induced hepatocyte reprogramming may be a promising strategy for generating functional hepatocytes and treating liver fibrosis caused by hepatic diseases.

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