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中华微生物学和免疫学杂志
北京生物制品研究所
中华微生物学和免疫学杂志

北京生物制品研究所

沈心亮

月刊

0254-5101

cjmia@163.com

010-52245168

100024

北京市朝阳区三间房南里4号

中华微生物学和免疫学杂志/Journal Chinese Journal of Microbiology and ImmunologyCSCD北大核心CSTPCD
查看更多>>中华医学会主办。本刊以医学微生物学和免疫学的基础和应用研究及其新理论、新技术以及国内外研究进展为报道内容。设有细菌学、病毒学、分子微生物学、临床微生物学、感染免疫、疫苗学、基础免疫学、临床免疫学、分子免疫学、免疫遗传、肿瘤免疫、中药免疫、免疫治疗、免疫学技术、检测技术、述评、综述、学术会议评述、人物述林、医药信息、新书介绍等栏目。读者为本专业科研人员、教师和高、中级卫生防疫、检验工作者及大学以上学生。本刊是中国生物科学和基础医学两学科核心期刊；被CA、BA、EM、Medline等国内外多种著名检索系统收录。杂志曾获国家优秀科技期刊奖并多次获省部级优秀科技期刊奖。2001年进入国家新闻出版署建设的“中国期刊方阵”。2008年获得中国科学技术信息研究所“中国精品科技期刊”称号。
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    外源性髓鞘抗原的清除在实验性自身免疫性脑脊髓炎中的作用

    郑配国曹雪珍魏旭锋
    67-73页
    查看更多>>摘要：目的 探讨外源性髓鞘抗原的清除对其诱导实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis, EAE)的作用。 方法 用髓鞘少突胶质细胞糖蛋白35～55肽(myelin oligodendrocyte glycoprotein 35-55，MOG35-55)或FITC标记的MOG35-55免疫C57BL/6J小鼠诱导EAE，通过分析过继转移的CFSE标记的mT/mG-2D2 CD4＋T细胞在脾脏内的增殖情况评估免疫系统内外源性髓鞘抗原的含量；免疫组织化学法和流式细胞术分析接种部位外源性髓鞘抗原的释放；组织切片HE染色探讨外源性髓鞘抗原快速清除的原因；通过对比分析背部和足垫免疫诱导的EAE，以及可溶性MOG35-55的治疗效果验证外源性髓鞘抗原的清除在EAE中的作用。 结果 免疫第2天小鼠脾脏内mT/mG-2D2 CD4＋T细胞增殖占比显著高于免疫第7天[(52.6±6.8)% vs(18.5±4.9)%, P<0.01]；在发病小鼠(第13天)脾脏内mT/mG-2D2 CD4＋T细胞增殖与在初始小鼠脾脏内的增殖差异无统计学意义[(4.4±1.5)% vs(2.5±1.4)%，P＝0.11]；免疫组织化学法和流式细胞术结果显示，MOG35-55在接种部位释放并被CD11b细胞吞噬，EAE发病时接种部位已无MOG35-55释放；组织病理切片显示，EAE发病时乳化剂周围形成的肉芽肿阻止乳化剂释放抗原，使抗原与外周免疫系统完全隔离；经足垫免疫诱导的小鼠不易发病与MOG35-55持续刺激免疫系统有关，与CD4＋调节性T细胞没有直接关系；持续腹腔注射可溶性MOG35-55可预防和治疗EAE。 结论 EAE小鼠免疫系统内外源性髓鞘抗原已完全清除，EAE的发生依赖外源性髓鞘抗原的完全清除。 Objective To investigate the role of the clearance of exogenous myelin antigen in experimental autoimmune encephalomyelitis (EAE). Methods EAE was induced in C57BL/6J mice by subcutaneous immunization with myelin oligodendrocyte glycoprotein 35-55 (MOG35-55) or FITC-MOG35-55. The concentration of exogenous myelin antigen was assessed by analyzing the proliferation of the transferred CFSE-labeled mT/mG-2D2 CD4+ T cells in spleen tissues. The release of exogenous myelin antigen from the inoculation sites was analyzed by immunohistochemistry and flow cytometry. HE staining was used to investigate the mechanism underlying the rapid clearance of exogenous myelin antigen. The role of the clearance of exogenous myelin antigen in EAE was investigated by comparative analysis of EAE induced by subcutaneous immunization in the back and footpads, and analyzing the therapeutic effect of soluble MOG35-55. Results The proliferation of mT/mG-2D2 CD4+ T cells in mice was enhanced on day 2 than on day 7 after immunization [(52.6±6.8)% vs (18.5±4.9)%, P<0.01]. There was no significant difference in the proliferation of mT/mG-2D2 CD4+ T cells between EAE mice (day 13) and naive mice [(4.4±1.5)% vs (2.5±1.4)%, P=0.11]. Immunohistochemistry and flow cytometry showed that MOG35-55 was released and engulfed by CD11b+ cells at the inoculation sites, and no more MOG35-55 was released at the onset of EAE. HE staining showed that granuloma that formed surrounding the antigen emulsion during EAE development prevented antigen release from the emulsion, completely isolating the antigen from the peripheral immune system. The incidence of EAE was relatively low in mice immunized via footpads, which was related to the sustained release of MOG35-55, but had no direct relation to CD4+ regulatory T cells. Continuous intraperitoneal injection of soluble MOG35-55 could prevent and treat EAE. Conclusions Exogenous myelin antigen has been completely cleared in EAE mice, and the occurrence of EAE depends on the clearance of the myelin antigen.

    自身抗原实验性自身免疫性脑脊髓炎CD4＋T细胞

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    分枝杆菌噬菌体D29 LysinB/Holin真核表达载体的构建及杀菌试验

    席志阳宋通王文涛吴文潇...
    74-80页
    查看更多>>摘要：目的 构建分枝杆菌噬菌体D29 LysinB/Holin融合蛋白真核表达载体，通过细胞感染模型研究其在胞内对结核分枝杆菌的杀伤效果。 方法 构建原核重组质粒pET32a-LysinB并诱导表达，纯化蛋白后制备多克隆抗体；构建真核重组质粒pcDNA3.1(＋)-LysinB/Holin并转染进单核巨噬细胞RAW264.7，经制备的LysinB多克隆抗体进行表达鉴定后，建立细胞感染模型并通过抗酸染色和菌落计数检测LysinB/Holin融合蛋白的杀菌效果。 结果 成功制备LysinB的多克隆抗体。重组质粒pcDNA3.1(＋)-LysinB/Holin在真核细胞中有效表达LysinB/Holin融合蛋白且对细胞无明显毒性。LysinB/Holin融合蛋白可有效杀伤胞内的结核分枝杆菌。 结论 重组质粒pcDNA3.1(＋)-LysinB/Holin对胞内结核分枝杆菌有较好的杀伤效果，且对细胞无明显毒性，具有治疗结核病的潜能。 Objective To construct a eukaryotic expression vector for bacteriophage D29 LysinB/Holin fusion protein and study its bactericidal efficacy against Mycobacterium tuberculosis (Mtb) in a cell infection model. Methods A recombinant plasmid pET32a-LysinB was constructed and induced to express LysinB. The polyclonal antibody against LysinB was prepared after the purification of LysinB. A recombinant plasmid pcDNA3.1(+ )-LysinB/Holin was constructed and transfected into mononuclear macrophages RAW264.7. After the expression of the prepared polyclonal antibody was identified, a cell infection model was established and the bactericidal efficacy of LysinB/Holin fusion protein was measured by acid-fast staining and colony counting. Results The polyclonal antibody against LysinB was successfully prepared. The recombinant plasmid pcDNA3.1(+ )-LysinB/Holin could effectively express LysinB/Holin fusion protein in eukaryotic cells without inducing significant cytotoxicity. LysinB/Holin fusion protein was effective in killing Mtb in cells. Conclusions The recombinant plasmid pcDNA3.1(+ )-LysinB/Holin has a better killing effect on intracellular Mtb without inducing obvious cytotoxicity against eukaryotic cells, showing a potential in the treatment of tuberculosis.

    结核分枝杆菌噬菌体D29LysinBHolin

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    烟曲霉锌稳态相关基因研究进展

    兰冠达贺丹王丽
    81-85页
    查看更多>>摘要：烟曲霉是一种通过空气传播的机会性致病真菌。其感染免疫功能低下患者肺部并引起严重的侵袭性曲霉病。锌是微生物生长所必需的微量元素，所有真菌包括烟曲霉生长都需要锌元素。有研究表明宿主体内的真菌毒力高低与真菌摄取锌的能力直接相关。由于元素锌在人体内大部分与锌结合蛋白结合导致在宿主组织微环境中的离子浓度远低于真菌最适生长浓度。烟曲霉演化出几种锌转运蛋白，使烟曲霉能够在锌缺乏的条件下有效吸收锌元素，同时抵抗锌元素过量，防止对烟曲霉细胞造成损害。本文将总结概述烟曲霉锌稳态调控对其生长发育及毒力的作用以及相关基因。明确烟曲霉对元素锌水平调控基因的研究现状并探讨干扰烟曲霉锌稳态是否会成为开发治疗侵袭性曲霉病的新一代辅助治疗措施和真菌治疗方案。 Aspergillus fumigatus is an opportunistic pathogenic fungus transmitted through the air. It targets the the lungs of immunocompromised patients and can cause severe invasive aspergillosis. Zinc, an essential trace element for microbial growth, is required by all fungi, including Aspergillus fumigatus. Some studies have shown a direct correlation between the virulence of fungi within the host and the uptake of zinc by them. However, zinc in the human body mostly binds to zinc-binding proteins, resulting in ion concentrations in the host′s tissue microenvironment much lower than the optimal growth concentration for fungi. There are several zinc transport proteins in Aspergillus fumigatus, enabling it to efficiently absorb zinc even in the condition of zinc deficiency. These proteins also protect the cells of Aspergillus fumigatu from the damage caused by excessive zinc. This article is to provide a comprehensive overview of the roles of zinc homeostasis regulation in the growth, development and virulence of Aspergillus fumigatus as well as the related genes, summarize the current research status of genes regulating zinc levels in Aspergillus fumigatus and investigate whether interference with zinc homeostasis in Aspergillus fumigatus will be a new generation of adjunctive therapy for invasive aspergillosis or antifungal strategy.

    烟曲霉锌稳态毒力药物靶点

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    抗感染免疫重点号导读

    F01页
    查看更多>>摘要：抗感染免疫是机体抵抗病原体入侵的重要防线，它涉及多种免疫细胞的活化和病原体的清除。随着研究的深入，我们对病原体的致病机制和各类免疫细胞在抗感染中的作用有了较为深入的了解。同时，新型检测技术的应用也为抗感染免疫研究提供了诸多手段。本刊特邀请吉林大学柳忠辉教授和崔雪玲教授担任本期"抗感染免疫"重点号执行编委，主要报道抗细菌免疫、抗病毒免疫，以及微流控技术在抗感染免疫研究中的应用等研究成果和进展。我们希望通过"抗感染免疫"重点号的策划和报道，能够为大家对抗感染免疫的理解和研究提供更多的借鉴和启发，同时也为抗感染药物的研发提供新的思路和方向。
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