International journal of peptide research and therapeutics.2022，Vol.28Issue(5) ：13.DOI:10.1007/s10989-022-10439-5

Antivenom Derived from the Ct1a and Ct17 Recombinant Toxins of the Scorpion Centruroides tecomanus

Melendez-Zempoalteca, Alejandra Juarez-Gonzalez, Victor Rivelino Rudino-Pinera, Enrique Pastor, Nina Vargas-Jaimes, Leonel Valcarcel-Gamino, Jose Antonio Vazquez-Vuelvas, Oscar Fernando Quintero-Hernandez, Veronica Valdez-Velazquez, Laura Leticia
International journal of peptide research and therapeutics.2022，Vol.28Issue(5) ：13.DOI:10.1007/s10989-022-10439-5
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Antivenom Derived from the Ct1a and Ct17 Recombinant Toxins of the Scorpion Centruroides tecomanus

Melendez-Zempoalteca, Alejandra 1Juarez-Gonzalez, Victor Rivelino 2Rudino-Pinera, Enrique 2Pastor, Nina 3Vargas-Jaimes, Leonel 2Valcarcel-Gamino, Jose Antonio 3Vazquez-Vuelvas, Oscar Fernando 1Quintero-Hernandez, Veronica 4Valdez-Velazquez, Laura Leticia1
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作者信息

  • 1. Fac Ciencias Quim,Univ Colima
  • 2. Inst Biotecnol,Univ Nacl Autonoma Mexico
  • 3. Ctr Invest Dinam Celular IICBA,Univ Autonoma Estado Morelos
  • 4. Ctr Invest Ciencias Microbiol Cicm,Benemerita Univ Autonoma Puebla BUAP
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Abstract

Scorpionism in Mexico is a public health problem caused by stings from the Centruroides scorpion family. The Ct1a and Ct17 toxins from the venom of the Centruroides tecomanus scorpion are the most abundant and toxic for mammals. This study describes the heterologous expression of recombinant proteins from genes encoding these toxins merged with thioredoxin in the vector pET-22b + and expressed in Escherichia coli BL21 (DE3). The yield of Ct1a and Ct17 recombinant toxins was 1.25 mg and 1.737 mg per liter of culture, respectively. These were purified by metal ion affinity chromatography and RP-HPLC and were used for immunization in rabbits, obtaining polyclonal antibodies that confer a positive immune response against the complete venom of Centruroides tecomanus. The serum was tested in vitro and in vivo, obtaining neutralization and protection against the venom. Both toxins were produced recombinantly and fused to the thioredoxin protein; remarkably, the recombinant toxins were excellent immunogens. 300 mu l of each immunized rabbit serum was tested in mice, resulting in 50% of the mice protected with each serum, but when the sera were pooled, the protection increased to 83%. This communication reveals the possibility of producing a specific and regional antivenom with polyclonal antibodies that neutralize the complete venom of Centruroides tecomanus. The sequence similarity of Ct1a and Ct17 to Cn2, a toxin that recognizes sodium channels, allowed in silico modeling analysis and a proposal for the different toxicities of Ct1a and Ct17.

Key words

Toxins/Scorpion/Centruroides/Heterologous expression/Antivenom/AMINO-ACID-SEQUENCE/VENOM PEPTIDES/DIVERSITY/ANTIBODY

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出版年

2022
International journal of peptide research and therapeutics.

International journal of peptide research and therapeutics.

ISSN：1573-3149
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