Role of autophagy in alpha particle-induced malignant transformation of human bronchial epithelial cells
OBJECTIVE:To investigate the role of autophagy in malignant transformation of immortalized human bronchial epithelial cells BEP2D induced by alpha particle radiation.METHODS:Western blot was used to detect the expression of autophagy related proteins(LC3B-II,LC3B-I and P62)in alpha particles exposed BEP2D and in exposed malignant transformed cell line BERP35T-1.Intracellular autophagosomes were observed by transmission electron microscopy.BERP35T-1 cells were also treated with 40 and 60 μmol/L autophagy inhibitor chloroquine(CQ)and 25 pmol/L autophagy activator Rapamycin(Rapa),respectively,for detection of of LC3Bs and P62.Survival rates of cells were measured by CCK-8,invasion ability by the Transwell invasion assay,and the migration ability by the scratch healing assay.RESULTS:Compared with BEP2D cells,the numbers of autophagosomes in RH24 and BERP35T-1 cells were increased,LC3B-II/I protein ratio increased,and P62 protein expression decreased.After treatment with 40 and 60 μmol/L CQ for 48 h,the LC3B-II/I protein ratio and the expression level of P62 protein in BERP35T-1 cells were increased,and the cell survival rate,invasion number and scratch closure rate decreased(all P<0.01).After treatment with 25 pmol/L Rapa for 48 h,the LC3B-II/I protein ratios were increased,the expression of P62 protein decreased,and the survival rate of BERP35T-1 cells,invasion number and scratch closure rate were increased(P<0.05 or 0.01).CONCLUSION:During the alpha particle-induced malignant transformation of BEP2D cells,autophagy was enhanced which was associated with increased ability of cell proliferation,invasion and migration.
autophagyalpha particlehuman bronchial epithelial cellscancerationlung cancer
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