Effects of benzo[b]fluoranthene and dibenzo[a,h]anthracene on oxidative stress and flammatory factors in rats
OBJECTIVE:To investigate effects of benzo[b]fluoranthene(BbFA)and dibenzo[a,h]anthracene(DahA)exposure on oxidative stress and inflammatory factors in rats.METHODS:After 1 week of acclimatization,48 adult male SD rats were randomly divided into exposed and control groups.The rats were exposed to BbFA and DahA by intratracheal instillation twice a week in 4 weeks.Inflammatory factors in BALF and serum,and oxidative stress factors in lung tissue and serum were determined.RESULTS:Serum activity of SOD was significantly decreased in the DahA-exposed groups(P<0.05).Meanwhile,the BbFA+DahA exposed groups showed significantly increased levels of MDA,NO,i-NOS in the lung tissue compared with the control group(P<0.05),and the levels of MDA and i-NOS in the BbFA+DahA combined exposure group were significantly higher than that of the BbFA-exposed and the DahA-exposed groups(P<0.05).Additionaly,the content of MDA in the BbFA+DahA combined exposure group was significantly higher than low dose group of BbFA-exposed group and DahA-exposed group(P<0.05).The levels of IL-6 in the BbFA+DahA combined exposure group were significantly up-regulated compared with the BbFA-exposed and the DahA-exposed groups(P<0.05).CONCLUSION:Our results demonstrated that BbFA and DahA caused changes in inflammatory and oxidative factors that were related to inhibition of SOD activity and elevation of iNOS activity,and MDA,NO and IL-6 levels.
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维普
