首页|CXCR2在食管癌组织中的表达及其对食管癌细胞生物学行为的影响

CXCR2在食管癌组织中的表达及其对食管癌细胞生物学行为的影响

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目的:探讨CXC受体2(CXCR2)在食管鳞癌组织中的表达及其对食管癌细胞生物学行为的影响.方法:收集手术切除的食管鳞癌组织74例作为研究组,其配对的癌旁正常食管组织74例作为对照组,采用免疫组织化学染色检测CXCR2的表达,比较研究组与对照组间CXCR2表达水平的差异,分析CXCR2表达及其与临床病理特征的关系.并采用CCK-8试剂盒、平板集落形成实验、细胞迁移和侵袭实验检测CXCR2拮抗剂SCH527123对食管癌细胞KYSE30生物学行为的影响.结果:与对照组比较,CXCR2在食管鳞癌研究组中的阳性表达率为73.0%(54/74),明显高于对照组13.5%(10/74),差异具有统计学意义(χ2=53.298,P=0.000).CXCR2的表达与食管鳞癌分化程度和淋巴结转移密切相关(χ2=5.515,P=0.019;χ2=7.320,P=0.007);与患者性别、年龄、肿瘤部位、肿瘤大体分型、肿瘤直径、浸润深度、脉管瘤栓及神经侵犯均无明显相关关系(P>0.05).在体外细胞实验中,与对照组相比,CXCR2拮抗剂SCH527123显著抑制食管癌细胞KYSE30的增殖、迁移和侵袭(均为P<0.05).结论:CXCR2在食管鳞癌组织中表达上调且与患者的不良预后相关,CXCR2拮抗剂SCH527123可以抑制食管癌细胞的增殖、迁移和侵袭,推测CXCR2可能是食管癌潜在的预测和靶向治疗的分子标志物.
Expression of CXCR2 in esophageal cancer tissues and its impact on biological behavior of esophageal cancer cells
OBJECTIVE:To investigate expression of the CXC receptor 2(CXCR2)in esophageal squamous cell carcinoma(ESCC)tissues and its impact on the biological behavior of esophageal cancer cells.METHODS:A total of 74 cases of surgically removed ESCC tissues were collected as the study group and 74 cases of paired adjacent normal esophageal tissues were collected as the control group.CXCR2 expression was detected by immunohistochemical staining,and the difference of CXCR2 expression level between the study and the control groups was compared,and the relationship between CXCR2 expression and clinicopathological characteristics was analyzed.The effects of CXCR2 antagonist SCH527123 on the biological behavior of esophageal cancer cells KYSE30 were examined by CCK-8 cell proliferation,plate colony formation,cell migration and invasion assay.RESULTS:The positive expression rate of CXCR2 in ESCC was 73.0%(54/74),which was significantly higher than the 13.5%(10/74)in the adjacent normal tissues(χ2=53.298,P=0.000).Significant differences were also detected in relation to the differentiation degree and lymph node metastasis of ESCC(χ2=5.515,P=0.019;χ2=7.320,P=0.007).However,the expression was not significantly related to gender,age,tumor location,gross classification of tumor,tumor diameter,depth of invasion,vessel emboli and nerve invasion(P>0.05).In the KYSE30 cells,application of the antagonist SCH527123 significantly inhibited their proliferation,migration,and invasion.CONCLUSION:CXCR2 expression was up regulated in ESCC tissues and was associated with poor prognosis of patients.CXCR2 antagonist SCH527123 inhibited the proliferation,migration,and invasion of esophageal cancer cells in vitro.CXCR2 may therefore be a molecular marker for molecular prediction and targeted therapy of esophageal cancer.

CXCR2esophageal squamous cell carcinomaclinical significancepathological featurestargeted therapybiological behavior

黄丛改、刘清、郑树涛、刘涛、谭依依、彭天元、陈娇、卢晓梅

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新疆医科大学第一附属医院,省部共建中亚高发病成因与防治国家重点实验室,新疆 乌鲁木齐 830011

CXCR2 食管癌 临床意义 病理特征 靶向治疗 生物学行为

国家自然科学基金新疆维吾尔自治区"天山英才"科技创新领军人才项目省部共建国家重点实验室项目省部共建国家重点实验室项目

822605682022TSYCLJ0031SKL-HIDCA-2022-14SKL-HIDCA-2020-JZ2

2024

10.3969/j.issn.1004-616x.2024.01.009

癌变·畸变·突变
中国环境诱变剂学会

癌变·畸变·突变

CSTPCD
影响因子：0.35
ISSN：1004-616X
年,卷(期)：2024.36(1)
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