摘要
目的:对细菌回复突变试验(Ames试验)中的菌株鉴定方法进行比较分析,以期为试验菌株鉴定提供参考.方法:根据Ames试验相关的国家和行业标准、技术规范和指导原则要求,对试验菌株的组氨酸缺陷型鉴定、脂多糖屏障缺陷(rfa突变)鉴定、氨苄青霉素抗性(R因子)鉴定、四环素抗性(pAQ1质粒)鉴定和uvrB修复缺陷型(紫外线敏感性)鉴定方法进行了分析对比.结果:鉴定结果表明,TA97a、TA98、TA100、TA102、TA1535等5株菌株生长均需组氨酸,均具有rfa突变,除TA1535外均具有氨苄青霉素抗性,除TA102外均对紫外线敏感和均无四环素抗性.结论:虽然鉴定方法不同,但是判定标准和鉴定结果相同,各个实验室应规定好试验菌株鉴定的方法和频率,为Ames试验结果的真实、可靠提供根本保障.
Abstract
OBJECTIVE:Compare and analyze the identification methods of test strains in bacterial reverse mutation assays(Ames test)for food,medical devices,cosmetics,chemicals,pesticides and drugs in China,in order to provide references for strain identification methods.METHODS:According to the requirements of national and the profession standard,technical standards and guidelines related to Ames tests,the identification methods of histidine auxotrophy,lipopolysaccharide barrier defect(rfa mutation),ampicillin resistance(R factor),tetracycline resistance(pAQ1 plasmid)and uvrB repair deficiency(sensitivity to ultraviolet light)were analyzed and compared.RESULTS:The results showed that TA97a,TA98,TA100,TA102 and TA1535 strains all required histidine for growth,all had rfa mutations,all had ampicillin resistance except TA1535,and all were sensitive to ultraviolet light and all had no tetracycline resistance except TA102.CONCLUSION:Although the identification methods were different,the criteria and identification results were the same.Each laboratory should specify the method and frequency of identification of test strains to provide fundamental guarantee for the authenticity and reliability of Ames test results.
基金项目
山东省人工噬菌体药物技术创新中心项目(ZY2019003)
维普
万方数据