Expression and significance of miR-544a in patients with colorectal cancer
OBJECTIVE:To explore expression level and clinical prognostic value of microRNA-544a (miR-544a) in tissues of colorectal cancer patients. METHODS:From January 2016 to February 2019, 150 patients with colorectal cancer were selected. Real time fluorescence quantitative PCR was used to detect expression levels of miR-544a in tissues, and immunohistochemistry was used to detect the expression of Ki67,CD4,and CD8 in tissues. Mann Whitney U-test,chi square test,and Fisher′s exact test were used to analyze relationships between miR-544a expression level in cancer tissue and clinical pathological indicators of patients. Univariate and multivariate Cox regressions were used to analyze factors affecting the prognosis of colorectal cancer. The Kaplan-Meier method was used to analyze relationships between expression levels of miR-544a in tissues and disease-free survival (DFS) and overall survival (OS) of patients. RESULTS:The relative expression level of miR-544a in colorectal cancer tissue (1.85 ± 0.15) was significantly higher than that in the adjacent normal tissues (1.06 ± 0.21) (P<0.01). The expression level of miR-544a was related to the incidence of peripheral nerve invasion,T staging,and N staging (all P<0.01),while the high expression group of miR-544a had a higher incidence of nerve invasion and a later T and N staging. The immunohistochemical results showed that the Ki67 score of the miR-544a high expression group was 120.2 ± 33.7, while the Ki67 score of the low expression group was 112.4 ± 40.8, with no significant difference between the two groups (P>0.05). The CD4 and CD8 scores of patients in the miR-544a high expression group were 15.5±5.3 and 24.8 ±7.6, respectively, lower than those in the miR-544a low expression group (21.3 ± 7.2 and 29.6 ± 8.9, respectively), with statistically significant differences (all P<0.05). Univariate and multivariate COX regression showed significant correlations between tumor N,M staging,and miR-544a expression levels and postoperative DFS and OS in CRC patients (all P<0.01). CONCLUSION:Upregulation of miR-544a expression in colorectal cancer tissue may be associated with delayed N and M stagings in colorectal cancer patients , and miR-544a may be a potential biomarker for immunotherapy efficacy.
万方数据
维普
