Expression of secreted frizzled-related protein 4 in hepatocellular carcinoma,and relationship with clinicopathological index and prognosis
OBJECTIVE:To investigate expression of secreted frizzled-related protein 4(sFRP4)in hepatocellular carcinoma(HCC)and relationship with clinicopathological index and prognosis.METHODS:Immunohistochemical methods were used to detect the expression of sFRP4 protein in 95 surgically resected HCC tissues and paracancerous tissues;patients clinicopathological indexes were used to analyze relationships with expression levels of the sFRP4 protein;the Kaplan-Meier survival curves were used to analyze relationships between expression of the sFRP4 protein and prognosis of the patients based on their clinicopathological index;and proportional hazards model(COX)was used to analyze factors affecting the prognosis of HCC patients.RESULTS:The positivity rate of sFRP4 protein was 48.42％(46/95)in HCC tissues and 85.26％(81/95)in paracancerous tissues.sFRP4 protein was therefore less positive in HCC tissues than in paracancerous tissues(x2=24.596,P＜0.01).Its expression in HCC tissues were significant for different histological grade(x2=11.877,P＜0.05),histological subtype(x2=3.873,P＜0.05)and microvascular invasion(x2=6.493,P＜0.05).Patients with the sFRP4 protein-negativity had significantly worse cumulative overall survival(OS)and cumulative disease-free survival(PFS)than that of the positive group(both P＜0.01).Cox multivariate analysis showed that sFRP4 protein[HR=0.238,95％CI(0.138,0.412),P＜0.01],clinical stage of hepatocellular carcinoma in China[HR=2.438,95％CI(1.256,4.730),P＜0.01]were independent risk factors affecting the PFS after surgical resection of HCC.In addition,the sFRP4 protein[HR=0.226,95％CI(0.109,0.467),P＜0.01]and histological grade[HR=2.236,95％CI(1.199,4.170),P＜0.05]were independent risk factors affecting the OS after surgical resection of HCC.CONCLUSION:Expression levels of the sFRP4 protein were differentially significant between the HCC and paracancerous tissues,as well as with histological grade,histological subtype and microvascular invasion in HCC tissues.The sFRP4 protein-negative group of patients had a poorer prognosis.In addition,its expression level was an independent risk factor for the prognosis of these patients after surgical resection.
hepatocellular carcinomasecreted frizzled-related protein 4clinicopathological indexprognosis
万方数据
维普
