Involvement of DKK-1 and NNMT in clinicopathology and prognosis among liver cancer patients
OBJECTIVE:To investigate the involvement of Dickkopf related protein 1(DKK-1)and nicotinamide N-methyltransferase(NNMT)in clinicopathology and prognosis among liver cancer patients.METHODS:Expression levels of NNMT and DKK-1 mRNA in normal liver tissues and liver cancer cells were analyzed by GEPIA2 database.,Effects of the two on the overall survival(OS)and disease-free survival(DFS)of patients with liver cancer were analyzed.Immunohistochemistry was used to detect the protein expression of NNMT and DKK-1 in liver tumor group,benign lesion group and normal group,and the relationship between them and the occurrence and development of liver cancer and clinicopathological features was analyzed.RESULTS:The GEPIA2 database showed that expression levels of NNMT mRNA in liver cancer tissues were significantly lower than that in normal liver tissues(P＜0.05).Expressions of NNMT and DKK-1 mRNA in liver cancers were negatively correlated(r=-0.13,P＜0.05).Both had no significant effect on the 10-year OS and DFS of liver cancer patients.The 5-year survival rate of liver cancer patients with high expression of NNMT mRNA was higher than that of those with low expression(P＜0.05).The results of immunohistochemistry showed that compared with normal liver tissues,the expressions of DKK-1 and NNMT proteins in liver cancer tissues were significantly reduced(P＜0.01).Expression of DKK-1 protein in hepatocellular carcinoma was significantly higher than that in cholangiocarcinoma.The expression of both proteins was not significantly correlated with tumor size,stage and tissue grade(P＞0.05).CONCLUSION:The expression levels of DKK-1 and NNMT proteins in liver cancer tissues are lower than those in normal liver tissues.The expression levels of DKK-1 protein were related to the tissue types of liver cancers,and the expression levels of NNMT mRNA affected the 5-year survival rate of patients with liver cancer.
万方数据
维普
