Teratogenicity and mutagenicity evaluation of refined fish oil
OBJECTIVE:To evaluation of teratogenicity and mutagenicity of refined fish oil(DHA content 89.97％,EPA content 1.12％).METHODS:SD female rats were given different doses(1.2,2.3,4.6 g/kg)of refined fish oil by oral administration on the 6th to 15th day of pregnancy.The body weight,pregnancy outcome,fetal viscera and bone,and fetal growth and development of pregnant rats were measured to evaluate the teratogenic effect of the fish oil.Effects of refined fish oil on the colony number and colony morphology of Salmonella typhimurium TA97a,TA98,TA100,TA102 and TA1535 were detected under activated and non-activated conditions.Its mutagenicity was evaluated by detecting the micronucleus rate in bone marrow cells and chromosome aberration rate in spermatogonia from normal ICR mice.RESULTS:In the teratogenic test,the NOAEL of refined fish oil on pregnant mice was 4.6 g/kg.The Ames test results for all dose groups(8-5 000 μg/dish)was negative under both metabolic and non-metabolic activation conditions.Compared with the control group,the micronucleus rate,chromosome aberration rate and aberration rate in bone marrow cells for each dose group were insignificant(P＞0.05),and there was no obvious mutagenicity.CONCLUSION:Under the experimental conditions,no teratogenic and mutagenic effects were observed in refined fish oil.
refined fish oilteratogenicitybacterial reverse mutationmicronucleuschromosome aberrationgenotoxicity
万方数据
维普
