Study on Alcohol Precipitation Process and Observation of Microstructure of Nicotinamide Mononucleotide
Using the property that nicotinamide mononucleotide is slightly soluble in alcohols,the process conditions for the separation and purification of nicotinamide mononucleotide by alcohol precipitation were optimized,and the stan-dard nicotinamide mononucleotide was obtained by vacuum drying.The content,purity,yield and moisture of nicotin-amide mononucleotide were used as indicators to investigate the factors such as alcohol precipitation temperature and al-cohol precipitation time.The optimum alcohol precipitation process and drying process of nicotinamide mononucleotide were determined.The optimum alcohol precipitation process of nicotinamide mononucleotide was obtained as follows:15 g/L nicotinamide mononucleotide solution was taken,ethanol was added to make the volume fraction of ethanol reach 70％,stirring at 4℃ for 1 h,and the alcohol precipitate was obtained by filtration.The yield of the product obtained by drying at-0.08 MPa and 50° C was the highest and the moisture and purity were up to standard.The reliability of the preparation of nicotinamide mononucleotide by this process was analyzed by scanning electron microscopy.The opti-mized process is stable and reliable,and can be used for the separation and purification of nicotinamide mononucleotide.
nicotinamide mononucleotidealcohol sedimentationmethod of dryingmicrostructure
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