Using the property that nicotinamide mononucleotide is slightly soluble in alcohols,the process conditions for the separation and purification of nicotinamide mononucleotide by alcohol precipitation were optimized,and the stan-dard nicotinamide mononucleotide was obtained by vacuum drying.The content,purity,yield and moisture of nicotin-amide mononucleotide were used as indicators to investigate the factors such as alcohol precipitation temperature and al-cohol precipitation time.The optimum alcohol precipitation process and drying process of nicotinamide mononucleotide were determined.The optimum alcohol precipitation process of nicotinamide mononucleotide was obtained as follows:15 g/L nicotinamide mononucleotide solution was taken,ethanol was added to make the volume fraction of ethanol reach 70％,stirring at 4℃ for 1 h,and the alcohol precipitate was obtained by filtration.The yield of the product obtained by drying at-0.08 MPa and 50° C was the highest and the moisture and purity were up to standard.The reliability of the preparation of nicotinamide mononucleotide by this process was analyzed by scanning electron microscopy.The opti-mized process is stable and reliable,and can be used for the separation and purification of nicotinamide mononucleotide.
nicotinamide mononucleotidealcohol sedimentationmethod of dryingmicrostructure
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