安徽化工2024,Vol.50Issue(2) :45-49,54.DOI:10.3969/j.issn.1008-553X.2024.02.011

微生物发酵法制备茶籽多肽及其功能活性研究

Preparation of Tea Seed Peptides by Microbial Fermentation and Its Functional Activities

张宇 陈胜现 黎玮 李婷 张钰 赵世光
安徽化工2024,Vol.50Issue(2) :45-49,54.DOI:10.3969/j.issn.1008-553X.2024.02.011

微生物发酵法制备茶籽多肽及其功能活性研究

Preparation of Tea Seed Peptides by Microbial Fermentation and Its Functional Activities

张宇 1陈胜现 2黎玮 2李婷 1张钰 1赵世光2
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作者信息

  • 1. 安徽工程大学生物与食品工程学院,安徽芜湖 241000
  • 2. 安徽工程大学生物与食品工程学院,安徽芜湖 241000;安徽省工业微生物分子育种工程实验室,安徽芜湖 241000
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摘要

采用微生物发酵茶籽制备茶籽多肽,并分析其功能活性及构效关系.结果表明:微生物发酵能提升茶籽原料的蛋白质成分释放及多肽生成,以解淀粉芽孢杆菌为最优发酵菌种,其最佳发酵工艺:接种量1%,发酵温度35℃,茶籽添加量50 g/L,pH 6.0,摇床转速120 r/min,发酵时间48 h,该条件下茶籽多肽产量达到6.13 mg/mL.茶籽原料经发酵后,小分子量蛋白比例提升了16.25%;茶籽多肽中的必需氨基酸、疏水性氨基酸及酸性氨基酸比例显著提升,使其抗氧化活性优于茶籽原料.

Abstract

The tea seed was used to produce peptides with fermentation by microbes and to analyze its functional activity. The results showed that microbial fermentation accelerated the release of proteins from tea seed and improved the genera-tion of peptides with the B. amyloliquefaciens as the optimal strain. The optimal fermentation parameters was obtained by single factor experiment as following: 1% of inoculum, 50 g/L of tea seed with 120 r/min of rotation at pH 6.0 for 48 h culti-vation, under which the tea seed peptide yield is up to 6.13 mg/mL. After fermentation, the proportion of proteins with low molecule increased 16.25%. The proportions of essential amino acids, hydrophobic amino acids and acid amino acids in-creased apparently, which promote the improvement of antioxidant capacity than raw tea seed.

关键词

茶籽多肽/微生物发酵/抗氧化活性/构效关系

Key words

tea seed peptides/microbial fermentation/antioxidant activity/structure-activity relationship

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基金项目

国家级大学生创新创业训练计划(202110363061)

安徽省重点研究与研发计划(202104a06020003)

安徽省工业微生物分子育种工程实验室开放基金(ELMB-06)

出版年

2024
安徽化工
安徽省化工研究院

安徽化工

影响因子:0.229
ISSN:1008-553X
参考文献量9
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