Study on Extraction Process and Antioxidant Activity of Total Phenols in Reed Leaves
In this study,with reed leaves from Hengshui Lake in Hebei Province,the total phenols were extracted by ultrasonic-assisted method.The extraction temperature,the ethanol concentration,the extraction time and the feed-to-liquid ratio were investigatedby single factor experiment accordingto absorbance of the total phenols in reed leaves.Based on the single factor experiments,the extraction technology was optimized by response surfacetest.The analysis of the experi-mental data showed that the extraction temperature was 48 ℃,the volumefraction of ethanol was 49%,the extraction time was 24 min,the feed-to-liquid ratio was 1∶67(mg/mL),the extraction rate of total phenols reached the highest.According to the analysis of the experimental data,it can be seen that the total flavone of reed leaves has a certain reduction ability,and the scavenging ability of free radicals is proportional to its concentration.