Optimization of Liquid Fermentation Conditions for Pigment Production of Monascus
Objective:To improve the ability of Monascus JTM-1 to produce pigment by optimizing the composition of liquid fermentation medium and fermentation conditions.Methods:Monascus JTM-1,isolated from red yeast rice,was selected as the experimental strain,and the liquid fermentation medium of Monascus JTM-1 was optimized by single factor experiment and orthogonal experiment.On this basis,the initial pH of fermentation,the amount of inoculation,the amount of liquid filling and the fermentation temperature were optimized.Results:The optimum composition of the fermentation medium was rice flour 50 g/L,peptone 14 g/L,KCl 0.5 g/L,K2HPO4 1 g/L,MgSO4·7H2O 0.25 g/L.The optimum fermentation condi-tions was initial pH 3.0,inoculation amount 8％,medium volume 25 mL/250 mL and fermentation temperature 28℃.Under this fermentation condition,the red and yellow values reached the maximum values,which were 29.68 AU/mL and 31.95 AU/mL,respectively.Conclusion:The optimum liquid fermentation medium and fermentation condition of Monascus JTM-1 were established to provide technical support for the large-scale production of monascus pigment.
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