Preparation and Its Hydrolysis Resolution Properties of Nanofiber D-Lacs@BC-NH2
D-Lactonohydrolase(D-Lacs)catalyzed hydrolysis and resolution is a new green catalytic technology for the preparation of pharmaceutical intermediate D-Lacs.However,free enzymes have problems such as high cost,easy deacti-vation,and difficulty in separation.This article uses a three-dimensional nanofiber network of coconut derived bacterial cellulose(BC)as a scaffold,modified with diethylenetriamine to BC-NH2,adsorbed with nanoenzymes,and immobilized with glutaraldehyde stabilized D-Lactonohydrolase in the BC network.The results showed that the amination modification improved the activity of BC immobilized enzyme.The enzyme load of BC-NH2 immobilized D-Lacs was up to 372.5 mg/g,and the hydrolysis and resolution of DL-pantolactone could be completed in about 4.5 hours,which were higher than un-modified BC.After 10 cycles of recycling,hydrolysis and separation experiments,it can be ensured that each batch reach-es half of the hydrolysis rate within 2 hours,it has been proven to have good cycling stability and is a promising technology for immobilization of all bio-based enzymes.
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