姜黄素/替莫唑胺脂质纳米粒的制备工艺研究
Preparation of Curcumin/Temozolomide Lipid Nanoparticles
陈静 1梅玥 1季颖 1陈璟 1吕志阳1
作者信息
- 1. 南京中医药大学翰林学院,江苏泰州 225300
- 折叠
摘要
目的:制备共载姜黄素(Cur)/替莫唑胺(TMZ)的脂质纳米粒(lipid nanoparticles,Cur/TMZ-LN).方法:用薄膜分散法、反复冻融法、载体沉积法等制备Cur/TMZ-LN,以包封率为评价指标选出最佳制备方法并进行单因素考查.结果:确定反复冻融法为最佳制备方法,且冻融次数为3,磷脂、胆固醇比为6∶1,磷脂浓度为3mg/Ml,姜黄素包封率为89.23％,替莫唑胺包封率为44.65％.结论:利用反复冻融法制备姜黄素/替莫唑胺脂质纳米粒,包封率较高,粒径较小.
Abstract
Objective:Prepare lipid nanoparticles (Cur/TMZ-LN) co-loaded with curcumin (Cur) and temozolomide (TMZ). Methods:Cur/TMZ-LN was prepared by thin film dispersion,repeated freezing-thawing and carrier deposition. The best preparation method was selected based on the encapsulation efficiency. Results:The method of repeated freez-ing-thawing was confirmed,and the times of freezing-thawing were 3,the ratio of phospholipid to cholesterol was 6∶1,the concentration of phospholipid was 3 mg/mL,the entrapment efficiency of curcumin was 89.23％,the entrapment effi-ciency of temozolomide was 44.65％. Conclusion:Curcumin/temozolomide lipid nanoparticles were prepared by repeated freezing-thawing method with high entrapment efficiency and small particle size.
关键词
姜黄素/替莫唑胺/脂质纳米粒/反复冻融法Key words
curcumin/temozolomide/lipid nanoparticles/repeated freeze-thaw method引用本文复制引用
基金项目
江苏省高校自然科学基金(21KJB360021)
江苏省高校自然科学基金(22KJB360014)
江苏省中医药科技发展计划项目(YB2020108)
泰州市社会发展支撑项目(SSF20220004)
泰州市社会发展支撑项目(SSF20230030)
2023年江苏省大学生创新创业训练项目(202313981013Y)
2023年江苏省大学生创新创业训练项目(202313981008Y)
2023年江苏省大学生创新创业训练项目(103152023059)
出版年
2024
NETL
NSTL
万方数据