To Explore the Best Extraction Process of Chlorogenic Acid by Reflux Extraction
Objective:In order to obtain the optimum process parameters of extracting chlorogenic acid from honeysuckle by reflux method. Methods:The single factor experiment and orthogonal experiment of extracting chlorogenic acid from honey-suckle by reflux method were designed. The single factor experiment used three factors of the four factors unchanged,and only changed A reflux temperature,B reflux time,C ethanol concentration and D liquid material ratio. On the basis of single factor experiment,the orthogonal experiment design of reflux method was carried out to investigate the effects of re-flux temperature (℃),reflux extraction time (h),ethanol concentration (％) and liquid-solid ratio (times) on the extraction rate of chlorogenic acid,so as to study the optimum technological parameters of extracting chlorogenic acid from honeysuckle in Guizhou by reflux method. Results:The results of single factor test of reflux extraction showed that the opti-mum temperature of reflux extraction of chlorogenic acid in honeysuckle was 70℃,the optimum time was 1.5 h,the opti-mum ethanol concentration was 70％,and the optimum liquid-solid ratio was 10 times. The influencing factors in the or-thogonal test were B>D>A>C. Conclusion:The optimum extraction parameters of chlorogenic acid by reflux extraction are A1B2C2D2,reflux extraction temperature is 60℃,extraction time is 1.5 h,ethanol concentration is 70％,and liquid-to-material ratio is 10 times. The optimization of the process parameters provides a theoretical basis and data support for the use of reflux extraction single factor test and orthogonal test to extract chlorogenic acid in Guizhou honeysuckle.
honeysucklechlorogenic acidrefluxing extractionorthogonal test
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