摘要
以石油醚、二氯甲烷、乙酸乙酯、乙醇、水超声辅助提取石椒草中不同极性部位的有效成分,采用抑菌圈实验和最低抑菌浓度(MIC)实验考查其抗菌活性,使用质谱对有效提取物中可能含有的化学成分进行表征.实验结果表明,乙醇、水、乙酸乙酯、二氯甲烷提取物对4种测试细菌(金黄色葡萄球菌、绿浓杆菌、枯草杆菌、大肠杆菌)具有抑制作用,抑菌圈直径为9.3～14.7mm.4种细菌对乙醇提取物最敏感,MIC值分别为12.5μg/mL、12.5μg/mL、25μg/mL和25μg/mL.所有提取物均未对真菌(白色念珠菌)显示出抑制作用.质谱图显示,乙醇提取物的特征信号峰为m/z 103.99、259.97、274.11、318.14、593.17,乙酸乙酯提取物的特征信号峰为m/z 259.97、274.11、318.14、503.36、588.30.结合已有报道,在有效提取物中识别出了2种香豆素、2种生物碱,石椒草有效提取物的抗菌活性可能来源于香豆素类和生物碱类化合物.
Abstract
Petroleum ether,dichloromethane,ethyl acetate,ethanol and water were used to prepare crude extracts of Boen-ninghausenia sessilicarpa. The diameter of antimicrobial zone and minimal inhibitory concentration (MIC) were determined to evaluate the antimicrobial activity against five strains including four bacteria strains (Staphylococcus aureus,Pseudomo-nas aeruginosa,Bacillus subtilis,Escherichia coli) and one fungal strain (Candida albicans). Use mass spectrometry to analyze potential chemical components in crude extracts. All extracts,except petroleum ether extracts,showed antimicro-bial activity against the four bacteria strains with inhibition zones from 9.3～14.7 mm. Four bacteria strains were most sensi-tive to ethanol extracts,the MIC values were 12.5 μg/mL,12.5 μg/mL,25 μg/mL,25 μg/mL,respectively. All extracts didn′t show antimicrobial activity against C. albicans. The mass spectrometry showed that the characteristic signal peaks of ethanol crude extracts were m/z 103.99,259.97,274.11,318.14,593.17,the characteristic signal peaks of ethyl acetate crude extracts were m/z 259.97,274.11,318.14,503.36,588.30. Based on the discovered components in Boenninghause-nia sessilicarpa,two coumarins and two alkaloids were identified in the effective extracts. The antimicrobial activity of the effective extracts may come from coumarins and alkaloids.
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