Antimicrobial Activity of Boenninghausenia sessilicarpa Extracts and Chemical Analysis by Mass Spectrum
Petroleum ether,dichloromethane,ethyl acetate,ethanol and water were used to prepare crude extracts of Boen-ninghausenia sessilicarpa. The diameter of antimicrobial zone and minimal inhibitory concentration (MIC) were determined to evaluate the antimicrobial activity against five strains including four bacteria strains (Staphylococcus aureus,Pseudomo-nas aeruginosa,Bacillus subtilis,Escherichia coli) and one fungal strain (Candida albicans). Use mass spectrometry to analyze potential chemical components in crude extracts. All extracts,except petroleum ether extracts,showed antimicro-bial activity against the four bacteria strains with inhibition zones from 9.3～14.7 mm. Four bacteria strains were most sensi-tive to ethanol extracts,the MIC values were 12.5 μg/mL,12.5 μg/mL,25 μg/mL,25 μg/mL,respectively. All extracts didn′t show antimicrobial activity against C. albicans. The mass spectrometry showed that the characteristic signal peaks of ethanol crude extracts were m/z 103.99,259.97,274.11,318.14,593.17,the characteristic signal peaks of ethyl acetate crude extracts were m/z 259.97,274.11,318.14,503.36,588.30. Based on the discovered components in Boenninghause-nia sessilicarpa,two coumarins and two alkaloids were identified in the effective extracts. The antimicrobial activity of the effective extracts may come from coumarins and alkaloids.
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