Determination of Malachite Green in Aquatic Products by Ultra-high Performance Liquid Chromatography Mass Spectrometry Coupled with PRiME HLB Solid Phase Extraction
Objective:The method for determinatiom Malachite Green(MG) and Leumalachite Green(LMG) in aquatic products by using Ultra-high Performance Liquid Chromatography Mass Spectrometry(UPLC-MS/MS)in combination with pass-through column was established.Method:The drugs in the sample were extracted with 1.5％ formic acid acetonitrile,centrifuged,and the extractionwas purified through a PRiME HLB solid-phase extraction column. After nitrogen blowing,it was redissolved and determined by UPLC-MS/MS,followed by quantitative analysis using isotope internal standard method. Results:The linear relationships of the drugs were good within the concentration range of 0.5～10 μg/L,with corre-lation coefficients greater than 0.995. The detection limit is 0.008～0.01 μg/kg,with a quantification limit of 0.02～0.04 μg/kg.The recovery rate for spiking is 86.8％～110.5％,with a relative standard deviation of 1.6％～5.8％. Conclusion:This method is simple,efficient,with good precision and accuracy,and can meet the requirements for the detection of Mala-chite Green and Leumalachite Green in large quantities of samples aquatic products.
Malachite Greenaquatic productsPRiME HLBultra-high perfoemance liquid chromatography mass spectrometry
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