Analysis on the Intestinal Bacterial Diversity of Maladera orientalis Damaging Camphora longepaniculata and Screening of the Functional Strains
In this paper, adults of Maladera orientalis damaging Camphora longepaniculata were used as the material and a approach combined with Illumina Miseq high-throughput sequencing and pure culture techniques was adopted to analyse the intestinal bacterial diversity of Maladera orientalis and community structure. Different function selecting mediums were used to screen out functional bacte-rial strains and enrich specific functional strain resources. Illumina Miseq sequencing showed that the intestinal bacteria of Maladera orientalis include 188 species belonging to 141 genera from 98 families in 57 orders under 25 classes of 15 phyla, with Firmicutes as the dominate phylum, unclassified Lachnospiraceae and Erysipelatoclostridium as the dominate genera. Analysis showed that the cul-turable intestinal bacteria include 128 strains belonging to 10 genera from 7 families in 6 orders under 5 classes of 4 phyla, with Prion as the dominant phylum and Serratia as the dominant genus. 19 protease producing strains, 13 esterase producing strains and 9 lipase producing strains were screened out with function selecting mediums. This study analyzed the intestinal bacterial diversity of Maladera orientalis and could lay a foundation for further understanding the role of the intestinal bacteria in regulating the interaction between Maladera orientalis and the host plant Camphora longepaniculata.
万方数据
维普
