Study on the Tissue Culture and Rapid Propagation Techniques for Hydrangea macrophylla'Masja'
In this paper,a study was made on the tissue culture and rapid propagation techniques for Hydrangea macrophylla'Masja'with stem segments having axillary buds as explants.The results showed that the axillary buds of the explants inoculated on the primary complete medium MS+0.5 mg/L 6-BA+0.02 mg/L NAA+0.1 mg/L GA3+25 g/L sucrose+6.5～7.5 g/L agar germinated in 6 d with an in-duction rate as high as 82％.The cluster buds could reach a multiplication coefficient of 12.1 when the advective buds were transferred to the enrichment medium MS+1.0 mg/L 6-BA+0.02 mg/L NAA+25 g/L sucrose+6.5～7.5 g/L agar,and most of the advective buds were vigorous,which could quickly take root and developed into a complete plant on the medium 1/2 MS+0.05～0.1 mg/L NAA and medium 0.2～0.3 g/L activated carbon.
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