Screening of pectinase producing Aspergillus strain,enzymatic properties of pectinase and its application in banana juice production
A filamentous fugal strain XYYWZSP4 producing pectinase was isolated from soil sample,and(0.78±0.01)U/mL of pectinase activity in culture broth was produced by using orange peel powder as sole carbon source.It was identified as Aspergillus sp.by observation of colony characters on Czapek medium plate and conidiophore.The enzyme property of its pectinase was determined as following:optimal pH at 4.5,optimal temperature at 60℃,stable at pH range of 3.0-8.0(with residual enzyme activity higher than 90%);stable at temperature not higher than 40℃.The pectinase had excellent metal ions compatibility with only inhibition effect of calcium at 5 mM among all tested metal ions.When the pectinase was applied in banana juice production,the juice yield of(89.85±1.57)%and reducing material concentration of(11.53±0.09)g/L were obtained.
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