Analysis of SSR loci in blueberry self-pollinated and cross-pollinated styles transcriptome
In order to carry out the identification of self-incompatibility genes in blueberry,transcriptome se-quencing was performed on the self-pollinated and cross-pollinated styles of blueberry cultivar"Premier"in this study,and simple repeat sequence(SSR)loci were analyzed.On this basis,SSR loci of assembled and redundant Unigene were analyzed,as well as it was conducted that the significant enrichment analysis of GO and KEGG for the differential ex-pression of unigenes containing SSR,and the SSR primers were designed.The results showed that a total of 85 890 unigenes were obtained from the blueberry styles transcriptome,of that 21 962 Unigene were found to contain 26 557 SSR loci,with a frequency of 25.57%.The mononucleotide and dinucleotide repeats were the main repeat types of the 297 kinds of repeat motif in all SSR loci,accounting for 89.52%of the total SSR,of which the number of A/T and AG/CT SSR locations accounted for 83.44%of the total number of SSR locations.A total of 17 212 pairs of primers were de-signed for all SSR loci,with a success rate of 78.37%.The differential transcripome combined with SSR loci information showed that 58,27,16,3,2 and 1 unigene with SSR sites were screened,which were related to plant hormone signal transduction,cytoskeleton,protein ubiquitination,pollen tube growth,ribonucnase T2 family,and programmed cell death,respectively.This study not only enriched EST-SSR markers of blueberry,but also laid a foundation for molecu-lar mechanism of self-incompatibility in blueberry.
万方数据
维普
