Analysis on the mechanism of artificial induction of awakening adaptation in Rana dybowskii
In order to explore the physiological mechanisms by which the Rana dybowskii adapts to temperature changes during artificial induction of awakening,including changes in cryoprotectant content at different temperatures and organs/tissues,as well as differences in antioxidant stress-related physiological indicators during the awakening adaptation stage,a gradient heating method was used to artificially induce awakening of the hibernating R.dybowskii,until the awakening process was marked by feeding by the R.dybowskii.During the awakening cultivation period,samples were taken,and non-sampled forest frogs were continuously cultured.After the sampling was completed,the remaining forest frogs were raised at room temperature;and the glucose content,urea nitrogen content,and total antioxidant capacity in its liver and muscles were measured using spectrophotometry.The results showed that the content of various substances in the liver was basically consistent with the trend of muscle consumption,but the content and changes of various substances in the liver were much greater than those in the muscle.The glucose and urea nitrogen content(cryoprotectant content)were significantly reduced(P<0.05),and the total antioxidant capacity was significantly enhanced(P<0.05)in the early stage of artificial induction and awakening of the hibernating R.dybowskii.However,in the later stage of awakening,the various contents tended to stabilize without significant changes.The liver is the main organ that fights against the low-temperature freezing environment during the hibernation process of the R.dybowskii,and the hibernating R.dybowskii accumulates cryoprotectants in its body.As the awakening process progresses,the temperature gradually rises,and the body activity gradually recovers.The glucose is consumed in large quantities,reducing stress damage caused by a lack of energy sources,and the antioxidant stress capacity is also enhanced to maintain internal environment stability,ensure the survival of R.dybowskii below 0℃celsius.
Rana dybowskiiartificial induction of awakeningfreeze protectantsantioxidant capacityartificial breeding
万方数据
维普
