Comparative analysis of volatile components in Cocos nucifera leaf under healthy and physically damaged conditions
Cocos nucifera leaf petiole volatiles exhibit an attractive effect on the Rhynchophorus ferrugineu,and exploring the volatile components of petioles under different states could analyzed the directional behavior of this insect.In order to elucidate the volatile components released from healthy and physically damaged Cocos nucifera leaf petioles,this study employed headspace sampling to collect the volatiles.Gas chromatography-mass spectrometry(GC-MS)was utilized to analyze the composition and relative abundance of volatile compounds,and a comparative analysis of the volatile components of healthy and physically damaged Cocos nucifera leaf petioles was conducted.The results indicated that a total of 53 volatile substances were detected in healthy petioles,including 26 hydrocarbons,2 nitriles,5 ketones,5 esters,6 indenes,5 alcohols and 4 other compounds.Among them,the 5 components with higher relative abundances were 4,9:5,8-dimethano-1H-benz[f]indene,3a,4,4a,5,8,8a,9,9a-octahydro-,Butylated Hydroxytoluene,L-.alpha.-Terpineol,Pyridazine,and Dicyclopentadiene.Physically damaged petioles emitted a total of 51 volatile substances,comprising 25 hydrocarbons,5 nitriles,5 ketones,6 esters,5 indenes,3 alcohols and 2 other compounds.Among them,the 5 components with higher relative abundances were 4,9:5,8-dimethano-1H-benz[f]indene,3a,4,4a,5,8,8a,9,9a-octahydro-,Butylated Hydroxytoluene,Bicyclo[2.2.2]oct-5-ene-2-carbonitrile,.alpha.-Terpineol,and 2,6-Dimethyl-4-oxatricyclo[5.2.1.0(2,6)]dec-8-en-3-one.The volatiles emitted from physically damaged petioles were generally similar to those from healthy petioles in terms of major components,but there were still some differences in specific types and relative abundances.The research results could provide a reference for elucidating the chemical communication mechanisms underlying the host-location behavior of the Rhynchophorus ferrugineus and for the development of plant-based attractants.