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加州鲈维氏气单胞菌拮抗菌的筛选

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为筛选出对维氏气单胞菌(Aeromonas Veronii)有拮抗效果的最优菌株,本试验从湖北荆州太湖加州鲈池塘底泥中分离获得一株细菌C2-1,对其进行全基因组测序,并研究其生防作用机制.结果表明,细菌C2-1为贝莱斯芽孢杆菌(Bacillus velezensis),具有广谱抑菌性,对希瓦氏菌(Shiva′s bacteria)、溶血链球菌(Streptococcus hemolysis)等多种水产病原菌的抑菌直径达18.57~25.28 mm.菌株C2-1具有较高的酸碱稳定性且对温度适应范围较为广泛.扫描电子显微镜镜观察发现细菌C2-1的发酵产物对维氏气单胞菌具有破坏效果.液质联用(LC-MS)分析该细菌包含多种不同的脂肽类活性物质,如伊枯草菌素等.研究发现,贝莱斯芽孢杆菌C2-1是防治维氏气单胞菌的潜力菌株,对维氏气单胞菌具有较好的抑制作用,且应用前景良好.
Screening of antagonistic bacteria against Aeromonas veronii in Micropterus salmoides
In order to select the best antagonistic strain against Aeromonas Veronii, a strain of bacteria C2-1 was isolated from the bottom mud of perch ponds in Tai Lake, Jingzhou, and whole genome sequencing, the biological control mechanism was studied. The results showed that the bacterial C2-1 was found to be Bacillus velezensis, with broad-spectrum antibacterial activity. Its antibacterial diameter against various aquatic pathogens such as Shiva′s bacteria and Streptococcus hemolysis ranged from 18.57 to 25.28 mm. The antibacterial substances of strain C2-1 had high acid-base stability and a wide range of temperature adaptation. Electron microscopy revealed that the fermentation products of bacterial C2-1 had a destructive effect on Aeromonas veronii. LC-MS analysis showed that the bacterium contains various lipopeptide active substances, such as imidacloprid. It was found that Bacillus velezensis C2-1 was a potential strain for the control of Aeromonas veronii and had good inhibition effect on Aeromonas veronii.

Aeromonas veroniiBacillus velezensisMicropterus salmoidesbiological control

安相茹、苏应兵、周燚

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长江大学动物科学技术学院,湖北荆州 434000

长江大学农学院,湖北荆州 434000

维氏气单胞菌 贝莱斯芽孢杆菌 加州鲈 生物防治

湖北省现代农业产业技术体系项目

2023-2

2024

安徽农学通报
安徽省农学会

安徽农学通报

影响因子:0.275
ISSN:1007-7731
年,卷(期):2024.30(13)