Optimization of DNA extraction methods from invasive plant Erigeron annuus
Erigeron annuus, which belongs to the genus Suaeda of Asteraceae family, is one of the most widely distributed and seriously harmful alien invasive weeds. To select a better method for extracting genomic DNA of E. annuus, the CTAB method for extracting genomic DNA of E. annuus was optimized by orthogonal experiment with four factors and three levels, and compared with the common plant kit method. The results showed that the modified CTAB method was used to extract genomic DNA of E. annuus. This involved treating the dried leaves of E. annuus with CTAB-free to remove polysaccharides, adding β-mercaptoethanol to prevent phenol oxidation, and extracting with chloroform∶isopentanol (24∶1) three times. The quality of the DNA extracted by the optimized scheme was good, with A260/A280 values measured by a microspectrophotometer close to 1.8 to 2.0, and the gel electrophoresis image band was clear without obvious drag. This method laid a foundation for studies on the genetic diversity and invasion mechanisms of E. Annuus.
AsteraceaeErigeron annuusDNA extraction methodmodified CTAB methodorthogonal test
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万方数据
