Effects of low temperature treatment on cold resistance of Phalaenopsis aphrodite
In order to explore the physiological mechanism of low temperature tolerance in Phalaenopsis aphrodite,this experiment used Da La Jiao plants of Phalaenopsis aphrodite varieties as experimental materials.After being treated with low temperature(5℃)for 1,3,5,and 7 hours,the chlorophyll,soluble sugars,soluble proteins,malondialdehyde(MDA)content,electrical conductivity,and A280 value in their leaves during the bud,blooming,and decline periods were measured.The results showed that with the extension of low-temperature treatment time,the total chlorophyll content showed an overall trend of first decreasing and then increasing.The changes in soluble protein content and soluble sugar content were not significant.The content of soluble protein showed a decreasing trend during the bud stage.The soluble sugar content during the decline period was higher than that during the bud and blooming periods.The MDA content,conductivity,and A280 slowly increased with the extension of low-temperature treatment time.The dynamic changes in chlorophyll,MDA,conductivity,and A280 ould reflect the degree of low-temperature loss in Phalaenopsis aphrodite,while the relationship between soluble sugars and soluble proteins and cold resistance is relatively small.
low temperature stressPhalaenopsis aphroditecold resistancemalondialdehydechlorophyll