Water Grass Tissue Culture Pollution Prevention and Control in experimental teaching reform
To explore the pollution sources and drug prevention measures of water plant tissue culture pollution,teaching was designed for the experimental training of water grass tissue culture pollution prevention and control,a teaching model was adopted that combined water grass tissue cell culture courses with water grass tissue culture pollu-tion prevention experiments,the teaching objectives and content were explored,and the experimental content and pro-cess were analyzed.Tissue culture on typical common water plant Hygrophila polysperma were conducted,by isolating and purifying the contaminating bacteria,16S rDNA and ITS gene sequencing were used in combination with phenotype analysis for species identification,clarifying the source of the contaminating bacteria.Further screening of prevention and control drugs for cotaminants was carried out,and effective drug combinations were obtained through drug sensitivity experiments.The addition of culture medium for tissue culture medium optimization achieved certain antibacterial and control effects.This course showed that this teaching model was beneficial for students to proficiently master the prin-ciples,schemes,and applications of water grass tissue culture pollution prevention and control technology,to cultivate professional talents for the expansion and production of aquatic plants and related scientific research.
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