Identification and biological characteristics of rice brown panicle disease
The diseased panicles of rice was isolated and purified in the experimental base of College of Agricultural,Shihezi University.The isolated pathogens was analyzed by morphology,ribosomal DNA internal transcribed spacers(rDNA-its)and histone 3 gene sequences,and the pathogenicity was determined,and its biological characteristics was studied.The results showed that Alternaria alternata was the pathogen causing brown lesions on rice panicles.The pathogenicity test showed that the rice panicles had the same symptoms as the sampled rice panicles,and the same Alternaria was isolated from the diseased panicle of rice.The optimum medium for the growth of the pathogen was sucrose as carbon source.The optimum pH 5-7.The optimum growth temperature 29-32 ℃.The optimum growth light 24 h full light.In conclusion,the rice brown panicle disease was caused by Alternaria alternata.
rice brown panicle diseaseAlternaria alternatamorphological identificationmolecular biological identificationbiological characteristics