Influences of three insecticides on virulence and detoxification enzyme activity of Helicoverpa armigera from sunflower fields
In order to screen effective insecticides for control Helicoverpa armigerain in sunflower field,the indoor toxicity of two chemical insecticides(200 g/L Chlorobenzamide and 10%Metre-indocarb)and one biocontrol agent(8 000 IU/mg Bacillus thuringiensis)to Helicoverpa armigera was determined by drop methodand.The effects of better control insecticide on the activities of acetylcholinesterase(AChE),carboxylesterase(CarE),cytochrome P450 enzyme(CYP450)and glutathione S-transferase(GST)were investigated.The results showed that the lethal concentration of 50%LC50 of 200 g/L Chlorobenzamide suspension 10%Metre-indocarb suspension,and 8 000 IU/mg Bacillus thuringiensis suspension on third instar of Helicoverpa armigera were 2.36,11.86 mg/L and 131.74 times,respectively,and the indoor insecticidal effect of Chlorobenzamide was the best.After treatment with Chlorobenzamide sublethal concentrations(LC25)for 12、24 and 48 h,the activity of AChE was significantly inhibited(P<0.05),while the activities of CarE and CYP450 were significantly enhanced(P<0.05),the activity of GST was not significantly changed(P>0.05).These results indicated that the detoxification metabolism of Helicoverpa armigera may be mainly through CarE and CYP450.The results provided references for the control of Helicoverpa armigera in sunflower field.
万方数据
维普
