Identification of salt resistance during germination of 40 cultivated foxtail millet varieties
To screen foxtail millet varieties with strong salt tolerance,Chenggu No.13 and other 40 foxtail millet varieties were treated with 150 mmol/L NaCl solution under salt stress,and the control group was distilled water.Six indicators including relative germination potential,relative germination index and relative germination rate of foxtail millet during germination were measured.The correlation analysis,principal component analysis,membership function analysis and cluster analysis were carried out.The results showed that the relative germination potential,germination rate and germination index of the Rehejinmi were the highest,the germination salt damage rate was the lowest,and the salt tolerance was strong.Correlation analysis showed that the relative germination potential was positively correlated with the relative germination rate and the relative germination index,the relative germination rate was positively correlated with the relative germination index,the relative germination index was positively correlated with the relative root length,and the relative root length was positively correlated with the relative bud length.Principal component analysis converted five indexes,including relative germination potential,into 2 principal components,and the cumulative contribution rate was 85.675%.The relative germination index and relative root length could be used as the main indexes to evaluate the salt tolerance of sorghum during germination.The comprehensive evaluation by the membership function method showed that the Rehejinmi had the largest D value and the strongest salt tolerance,while that from Chengsi No.9 had the smallest D value and the worst salt tolerance.The 40 foxtail millet varieties were divided into 4 categories by cluster analysis.Among them,the salt-tolerant varieties Rehejinmi,the salt-tolerant varieties 20-M919 and 20-M841,the moderately salt-tolerant varieties included Shanxihonggu,Yugu No.1 and Chenggu No.15,and the salt-sensitive varieties included 29 varieties such as Chengsi No.9,Chenggu No.20,and Chenggu No.14.The results provide a reference basis for foxtail millet planting in saline-alkali land.