Effects of different LEDs light quality on physiological characteristics of Abelmoschus esculentus tissue culture seedlings
To select the optimal light quality suitable for tissue culture seedlings of Abelmoschus esculentus,Wufu variety was used as experimental material,and a single factor randomized block design experiment was carried out.The terminal bud explants(1.5 cm)were inoculated on 1/2 MS basic medium.Under fluorescent(CK),blue(B),red(R),yellow(Y),green(G)and far red(FR)light irradiation,fresh sample weight,dry sample weight,root activity,photosynthetic pigment content such as chlorophyll a,antioxidant enzyme activity such as SOD and carbohydrate content such as sucrose were determined.The results showed that under each light quality treatment,the change trend of fresh weight and dry weight was consistent,and B treatment was the highest,which were 2.54 g and 0.46 mg,respectively.The root activity of seedlings under B treatment was the highest.The chlorophyll a,chlorophyll b,total chlorophyll,and carotenoid contents of tissue culture seedlings treated with B were the highest,which were 1.09,0.48,1.47,and 0.38 mg/g,respectively,and the chlorophyll a/b contents under CK treatment were higher.The activities of SOD and POD in leaves were the highest under B treatment,which were 362.75 and 189.69 U/(g·min),respectively.The contents of sucrose,starch,soluble sugar,and total soluble sugar in leaves were the highest under R treatment.In conclusion,monochrome blue light treatment can effectively improve the quality of fresh samples,dry samples,root activity,and photosynthetic pigment content in leaves;both monochrome red and blue light treatment can promote the accumulation of carbohydrates such as sucrose in leaves.Therefore,the monochromatic blue light can be used as the monochromatic light quality in vitro culture of Abelmoschus esculentus.This study provides references for the utilization of light in the rapid propagation of Abelmoschus esculentus in vitro.
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