Characterization analysis of microsatellite in chloroplast of Spiraea cantoniensis Lour.
To investigate the characteristics of simple repeat sequences(SSR)in the chloroplast genome of Spiraea cantoniensis Lour.,based on the chloroplast genome sequences of Spiraea cantoniensis Lour.collected from GenBank databases,the chloroplast genome characteristics,scattered repeat sequences,and chloroplast microsatellites(cpSSR)features were analyzed using REPuter and MISA software.The results showed that the complete chloroplast genome sequence was 155 932 bp,and it is a typical tetrad structure;43 interspersed repeat sequences were identified,with the most palindromic repeats and the least complementary repeats.A total of 57 cpSSR loci were identified on the chloroplast genome sequence,mainly distributed in the LSC region.There were 4 types of cpSSR repeat base compositions,namely mononucleotide,dinucleotide,tetranucleotide,and pentanucleotide.Among them,mononucleotide repeat sequences were the most numerous with 45,which accounted for 78.95%of the total repeat sequences,and pentanucleotide repeat sequences had the least,only 1,which accounted for 1.75%of the total repeat sequences.The length range of cpSSR was 10-19 bp,with the highest length being 10 bp,accounting for 43.86%;the copy number of cpSSR motifs in the chloroplasts of Spiraea cantoniensis Lour.varied from 3 to 19,with the most sequences having a copy number of 10,with 25,accounting for 43.86%of the total repeats.This experiment investigated the distribution patterns and characteristics of chloroplast microsatellites of Spiraea cantoniensis Lour.,providing a reference for species identification,population genetics,and phylogenetic research of Spiraea.
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万方数据
